全文获取类型
收费全文 | 8564篇 |
免费 | 1083篇 |
国内免费 | 403篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 117篇 |
妇产科学 | 48篇 |
基础医学 | 323篇 |
口腔科学 | 92篇 |
临床医学 | 562篇 |
内科学 | 770篇 |
皮肤病学 | 95篇 |
神经病学 | 387篇 |
特种医学 | 79篇 |
外科学 | 739篇 |
综合类 | 2006篇 |
预防医学 | 501篇 |
眼科学 | 81篇 |
药学 | 1866篇 |
4篇 | |
中国医学 | 1957篇 |
肿瘤学 | 405篇 |
出版年
2024年 | 104篇 |
2023年 | 429篇 |
2022年 | 758篇 |
2021年 | 880篇 |
2020年 | 698篇 |
2019年 | 478篇 |
2018年 | 362篇 |
2017年 | 328篇 |
2016年 | 251篇 |
2015年 | 257篇 |
2014年 | 434篇 |
2013年 | 473篇 |
2012年 | 399篇 |
2011年 | 404篇 |
2010年 | 328篇 |
2009年 | 263篇 |
2008年 | 311篇 |
2007年 | 359篇 |
2006年 | 334篇 |
2005年 | 328篇 |
2004年 | 235篇 |
2003年 | 362篇 |
2002年 | 171篇 |
2001年 | 203篇 |
2000年 | 123篇 |
1999年 | 89篇 |
1998年 | 81篇 |
1997年 | 81篇 |
1996年 | 57篇 |
1995年 | 73篇 |
1994年 | 57篇 |
1993年 | 41篇 |
1992年 | 34篇 |
1991年 | 41篇 |
1990年 | 27篇 |
1989年 | 27篇 |
1988年 | 19篇 |
1987年 | 19篇 |
1986年 | 20篇 |
1985年 | 13篇 |
1984年 | 15篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 10篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 8篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1971年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
开心胶囊对大鼠垂体后叶素心肌缺血模型的影响 总被引:2,自引:0,他引:2
临床发现,由西洋参,川苍,苍术,香附,蒲黄,红花,山楂等组成的呀约开心胶囊对心肌缺血有改善作用。为探讨其作用机理,建立垂体后叶素(Pit)造成的心肌缺血大鼠模型,观察中药开心胶囊不同剂量对心肌缺血大鼠的防治作用,并以肠溶阿斯匹林作阳性对照,结果中药开心胶囊高剂量组可改善Pit引起的心肌缺血并提高大鼠6-酮-前列腺素F1α-(6-Keto-PGF1α)的含量(与模型组比P〈0.01);中、高剂量组可 相似文献
102.
脑力智宝对PC12细胞缺血性损伤的保护作用 总被引:10,自引:0,他引:10
用NaCN加缺糖造成的PC12细胞缺血性损伤模型,研究脑力智宝含药血清对细胞的保护作用。并对所取含药血清时不同的给药次数,采血时间和含药血清加入量等实验方法进行了探讨。结果表明,单次给药后0.5、1.2、3h所采集的含药血清不具有细胞保护作用,而两次给药(间隔2h)后1h,2h的含药血清具有明显的保护作用。每日两次给经连续3.5d(7次给药)各时间点的含药血清均有明显的细胞保护作用。血清加入量以5 相似文献
103.
EFFECTS OF RADIX ANGE LICAE SINENSIS AND SHUANGHUANGLIAN ON A RAT MOD EL OF CHRONIC PSEUDOMONAS AERUGINOSA PNEUMONIA 总被引:2,自引:0,他引:2
INTRODUCTION Incysticfibrosis (CF)patients,Pseudomonasaerugi nosa (PA)isthemajorpathogencausingchroniclunginfec tionwithincreasingage (1,2 ) .Theinfectionresultsinaremarkableantibodyresponse ,leadingtoformationofim munecomplexesfollowedbyinfiltrationofpolymor… 相似文献
104.
Huang P 《The International journal of health planning and management》1995,10(3):183-191
This paper examines the hospital accreditation system in Taiwan, Republic of China. The paper describes the historical evolution of accreditation; preliminary teaching hospital accreditation; hospital and teaching hospital accreditation; issues and problems; and perspectives and challenges. 相似文献
105.
BackgroundMindfulness interventions can improve psychological distress and quality of life (QoL) in cancer survivors. Few mindfulness programs for cancer recovery exist in Southeastern U.S. The primary objective of this study was to assess the feasibility of a modified mindfulness-based stress reduction (MBSR) program for breast cancer patients in Birmingham, Alabama. The secondary objective was to explore potential program effects on mindfulness skills and QoL.MethodsThis study was a prospective, quasi-experimental feasibility study conducted over 10 months at a university hospital. Feasibility was achieved if 80% of eligible patients screened enrolled in the study and 70% of enrolled patients attended all 8 program sessions. Effectiveness was estimated by changes in mindfulness and QoL indicators measured with validated scales administered at 3 time points and assessed with a non-parametric Friedman test. Sessions included meditation, yoga, and an attention practice called body scan. There were 3 groups of 2–5 patients.ResultsThe sample totaled 12 patients. Forty-four percent (12/27) of eligible patients enrolled in the study, and two out of 12 enrolled patients completed 8 program sessions, resulting in 16.7% (2/12) retention. However, more than half (66.7%) of participants completed at least 7 sessions. Between baseline and 8-week follow-up, patients demonstrated statistically significant improvements in distress, general wellbeing, and fatigue-related QoL.ConclusionsFeasibility objectives were not achieved. However, a majority of participants (66.7%) completed 7 of 8 program sessions. Program effects were promising for distress, fatigue, and wellbeing. Results warrant further research on MBSR-like programs for breast cancer patients in Alabama. 相似文献
106.
虎杖蒽醌化合物抗CVB3病毒的实验研究 总被引:10,自引:0,他引:10
王志洁 《成都中医药大学学报》1999,22(2):41-43
目的:为了解虎杖蒽醌化合物晶Ⅰ,晶Ⅳ抗CVB3病毒的药效从而明确虎杖的抗病毒性能并开发利用。方法;以病毒唑为阳性对照,利用空斑减数实验进行研究。结果:在HEP-2细胞系统中,晶Ⅰ对CVB3直接杀灭,增殖抑制,感染阻断ED50分别为1.54μg/mL,8.34μg/mL,3.06μg/mL,晶Ⅳ的相 系数分别为1.79μg/mL,7.05μg/mL,3.17μg/mL,而病毒唑的相应系数分别为6.2 相似文献
107.
目的:观察银杏叶总黄酮(TFGb)抗心肌缺血的作用。方法:采用结扎家兔冠状动脉前降支造成急性心肌梗塞的病理模型,用心电图、血清磷酸肌酸激酶活性和梗死面积评价TFGb对心肌缺血的保护作用。结果:TFGb(167mg·kg-1·d-1,ip,连续用药14d)可明显降低心肌梗塞兔EKG中ST段异常抬高的总幅度以及病理性Q波的出现数;并显著抑制心肌组织磷酸肌酸激酶释放。硝基四氮唑蓝染色显示,预先用TFGb可使心肌梗塞范围明显缩小。结论:TFGb对心肌缺血性损伤具有保护作用 相似文献
108.
Extracellular 4-aminopyridine (4-AP), tetraethylammonium chloride (TEA) and quinine depolarized the neuropile glial cell membrane and decreased its input resistance. As 4-AP induced the most pronounced effects, we focused on the action of 4-AP and clarified the ionic mechanisms involved. 4-AP did not only block glial K+ channels, but also induced Na+ and Ca2+ influx via other than voltage-gated channels. The reversal potential of the 4-AP-induced current was -5 mV. Application of 5 mM Ni2+ or 0.1 mM d-tubocurarine reduced the 4-AP-induced depolarization and the associated decrease in input resistance. We therefore suggest that 4-AP mediates neuronal acetylcholine release, apparently by a presynaptic mechanism. Activation of glial nicotinic acetylcholine receptors contributes to the depolarization, the decrease in input resistance, and the 4-AP-induced inward current. Furthermore, the 4-AP-induced depolarization activates additional voltage-sensitive K+ and Cl- channels and 4-AP-induced Ca2+ influx could activate Ca2+-sensitive K+ and Cl- channels. Together these effects compensate and even exceed the 4-AP-mediated reduction in K+ conductance. Therefore, the 4-AP-induced depolarization was paralleled by a decreasing input resistance. 相似文献
109.
Roccamo AM Pediconi MF Aztiria E Zanello L Wolstenholme A Barrantes FJ 《The European journal of neuroscience》1999,11(5):1615-1623
The properties of the nicotinic acetylcholine receptor (AChR) are modulated by its lipid microenvironment. Studies of such modulation are hampered by the cell's homeostatic mechanisms that impede sustained modification of membrane lipid composition. We have devised a novel strategy to circumvent this problem and study the effect of changes in plasma membrane lipid composition on the functional properties of AChR. This approach is based on the stable transfection of AChR subunit cDNAs into cells defective in a specific lipid metabolic pathway. In the present work we illustrate this new strategy with the successful transfection of a temperature-sensitive Chinese hamster ovary (CHO) cell line, SPB-1, with the genes corresponding to the four adult mouse AChR subunits. The new clone, SPB-1/SPH, carries a mutation of the gene coding for serine palmitoyl transferase, the enzyme that catalyses the first step in sphingomyelin (Sph) biosynthesis. This defect causes a decrease of Sph de novo synthesis at non-permissive temperatures. The IC50 for inhibition of alpha-BTX binding with the agonist carbamoylcholine exhibited values of 3.6 and 2.7 microm in the wild-type and Sph-deficient cell lines, respectively. The corresponding IC50 values for the competitive antagonist D-tubocurarine (D-TC) were 2.8 and 3.4 microm, respectively. No differences in single-channel properties were observed between wild-type and mutant cell lines grown at the non-permissive, lipid defect-expressing temperature using the patch-clamp technique. Both cells exhibited two open times with mean values of 0.35 +/- 0.05 and 1.78 +/- 0.2 ms at 12 degrees C. Taken together, these results suggest that the AChR is expressed as the complete heteroligomer. However, only 10-20% of the total AChR synthesized reached the surface membrane in the mutant cell line and exhibited a higher metabolic turnover, with a half-life about 50% shorter than the wild-type cells. When control CHO-K1/A5 cells were treated with fumonisin B1, an inhibitor of sphingosine (sphinganine) N-acetyltransferase (ceramide synthase), a 45.5% decrease in cell surface AChR expression was observed. The results suggest that sphingomyelin deficiency conditions AChR targeting to the plasma membrane. 相似文献
110.
中药骨康对去睾大鼠骨质疏松的防治作用 总被引:4,自引:0,他引:4
为探讨补肾活血类中药骨康口服液对去睾大鼠所造成的骨质疏松的影响 ,通过切除大鼠睾丸造骨质疏松模型 ,观察了不同剂量中药骨康口服液对该模型大鼠骨面密度 ( ρA ,bone)、骨矿含量 (mM ,bone)及股骨骨生物力学的影响 ,并与特乐定作阳性对照。结果 :中药骨康高、中剂量防治的去睾大鼠股骨扭转试验最大扭矩及血睾酮 (T)含量 ( ρT ,bl)高于模型组 (P <0 .0 5和P <0 .0 1) ,高、中、低剂量组全身及左、右股骨ρA ,bone、mM ,bone高于模型组 (P <0 .0 5 )。提示骨康口服液能提高去睾大鼠血睾酮含量、骨的骨矿含量及骨密度 ,改善骨组织的生物力学性能 ,避免骨折的发生。为中药骨康口服液防治老年男性骨质疏松症提供实验依据。 相似文献