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101.
M Mottolese A Cianciulli I Venturo R Perrone Donnorso M Salzano M Benevolo G Bigotti P G Natali 《Diagnostic cytopathology》1992,8(2):153-160
The use of a selected panel of monoclonal antibodies (MoAb) to tumor associated antigens (TAA) in immunocytochemical (IIC) tests has been shown, in a preliminary study, to be a powerful diagnostic tool for the identification of the primary solid tumor causing metastatic effusion. Despite this improvement in a minority of neoplastic fluids a number of different causes may still determine false negative (FN) immunocytochemical diagnoses. The aim of the present study was to confirm the diagnostic accuracy of this panel of MoAb. This was done by analyzing in IIC tests a larger number of effusions and by evaluating whether the expansion in short term culture of those fluids with an uncertain malignant morphology could provide an adequate cellular substrate for immunocytodiagnosis. The analysis of 314 effusions confirmed the results of the pilot study and demonstrated that the combination of short term culture and immunocytochemical assays can further increase the sensitivity of this novel diagnostic procedure from 84.3% to 95.3%. 相似文献
102.
J. HIRSCH 《Anaesthesia》1994,49(1):30-33
103.
Senile plaques in Alzheimer's disease (AD) are composed principally of Aβ, a 4 kDa fragment of the amyloid precursor protein (APP). Longer forms of APP which contain a Kunitz proteinase inhibitor (KPI) domain are elevated in aged and in AD brains. Tissue factor pathway inhibitor-1 (TFPI) contains three tandem KPI domains and has been well characterized for its role as a natural anticoagulant in the extrinsic coagulation pathway. Functionally, the first two KPI domains of TFPI bind and inhibit the activity of factor Xa and VIIa respectively. In addition, TFPI and APP-KPI share a common clearance mechanism through the low density lipoprotein receptor-related protein (LRP). As part of an ongoing study of the role of KPI-containing proteins in AD, the current study examines TFPI localization in the brain. We report here that TFPI is immunohistochemically localized to microglia in both AD and non-AD individuals and is localized to some senile plaques in AD. Western blot analyses indicate that the amount of TFPI is elevated in frontal cortex samples from AD brains. We propose that TFPI may play a cell specific role in proteinase regulation in the brain. 相似文献
104.
硫酸镁治疗新生儿重症肺炎的临床研究及对血气血镁的影响 总被引:1,自引:0,他引:1
目的 为探讨硫酸镁治疗新生儿重症肺炎的临床效果及对血气血镁的影响。方法 采用 96例新生儿重症肺炎患儿随机分成治疗组 4 8例 ,对照组 4 8例 ,治疗组加用硫酸镁 30~ 5 0mg/(kg·d)加入 5 %葡萄糖 2 0ml静滴 ,持续 1h ,疗程3~ 8d ,同时监测血气血镁。结果 治疗组总有效率 79.2 % ,对照组总有效率 5 0 .0 % (P <0 .0 1) ,硫酸镁在改善症状 ,消除肺部体征及缩短住院时间和提高治愈率等方面优于对照组 ,治疗组病死率下降 ,同时血气恢复正常优于对照组 ,血镁含量治疗前后无变化。结论 硫酸镁治疗新生儿重症肺炎疗效显著 ,未发现明显副作用。 相似文献
105.
106.
107.
全身麻醉插管术后院内获得性肺部感染探讨 总被引:17,自引:3,他引:14
全身麻醉气管插管术后下呼吸道感染具有较高发生率。分析526例手术时间超过4小时普外科病例,经u检验发现全身麻醉气管插管术后患者肺部感染率明显高于持续性硬膜外麻醉术后患者,影响因素主要与全麻插管用具及麻醉机消毒情况有关,也与术前存在慢性阻塞性肺部疾患和术后保留气管插管有关。降低全麻术后肺部感染应重视无菌操作,尽可能对麻醉机管道进行常规消毒。 相似文献
108.
Shilan JIN Tetsuya OOTAKA Jun SOMA Toshinbbu SATO Hiroshi SATO Sadayoshi ITO Takao SAITO 《Nephrology (Carlton, Vic.)》2002,7(4):189-197
SUMMARY: A comparative immunohistological study was performed for the glomerular deposition of complements (C1q and C3c), fibrin/fibrinogen‐related antigen (FRA), the expression of intercellular adhesion molecule‐1 (ICAM‐1), and the infiltration of leucocytes bearing β2 integrins (leucocyte function associated antigen‐1 (LFA‐1), complement receptor 3 (CR3) and complement receptor 4 (CR4)) on renal biopsy specimens from 49 cases with Henoch‐Schoenlein purpura nephritis (HSPN), and 49 age‐matched cases with immunoglobulin A nephropathy (IgAN). the glomerular expression of ICAM‐1 was signifcantly correlated with the glomerular infiltration of leucocyte function associated antigen (LFA)‐1+ leucocytes in both diseases, and with that of CR3+ leucocytes in HSPN. the expression of ICAM‐1 was closely localized with the infiltration of LFA‐1+ leucocytes in the study with double immunostaining. the incidence and intensity of glomerular deposition of FRA were significantly higher in HSPN than in IgAN (P< 0.001), and those of C3c were significantly lower in HSPN than in IgAN (P< 0.001). the glomerular deposition of FRA was significantly correlated with the glomerular infiltration of CR4+ leucocytes in HSPN (P<0.05) but not in IgAN. In contrast, the glomerular deposition of C3c was significantly correlated with the glomerular infiltration of CR4+ leucocytes in IgAN (P<0.05), but not in HSPN. Studies with double immunostaining revealed a close association of CR4+ leucocytes with FRA deposition in HSPN and with C3c deposition in IgAN, respectively. the number of glomerular leucocytes bearing β2 integrins was significantly correlated with urinary protein at the time of renal biopsy in both diseases. These results suggested the differential roles of β2 integrins in the induction of glomerular injury in HSPN and IgAN. the ICAM‐1/LFA‐1 interaction may commonly be involved in the glomerular infiltration of leucocytes in both diseases. the ICAM‐1/CR3 interaction may be involved only in HSPN. Complement receptor 4 may function as a fibrin/fibrinogen receptor in HSPN, while CR4 may function as a complement receptor in IgAN. 相似文献
109.
采用动脉血氧饱和度仪对52例新生儿肺炎患儿,在雾化吸入治疗过程中的动脉血氧饱和度(SaO2)进行了监测,对比了吸氧与否对SaO2改变的影响。结查表明:与基础值比较,雾化吸入时患儿的SaO2显著下降(P<0.01),而雾化吸入同时吸氧者SaO2无明显变化(P>0.05),但两者比较具有显著性差异(P<0.01)。结果提示雾化吸入可使新生儿肺炎患儿SaO2下降;同时吸氧,对防止SaO2的下降有一定作用。 相似文献
110.
为了探讨肺炎克雷白杆菌的基因分型,我们用煮沸法制备细菌基因组DNA,进行随机引物-聚合物酶链反应,所有18个肺炎克雷白杆菌菌株均产生了特异性的,可重复的扩增条带,提示:RAPD方法可用于肺炎克雷白杆菌的分型鉴定和进行分子流行病学研究。 相似文献