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81.
F. C. Gwazdauskas C. McCaffrey T. G. McEvoy J. M. Sreenan 《Journal of assisted reproduction and genetics》1992,9(2):149-154
Embryos from two strains of mice were used to assess the effect of incubation temperature on pronuclear and twocell development to the morula/blastocyst (M/B) stage. Embryos from B6D2F2 and B6SJLF1 strains were cultured in medium M16 at either 37 or 39°C until 120 hr post human chorionic gonadotropin (hCG) or 0, 24, or 48 hr at 37°C and the remaining time at 39°C. Overall M/B development for pronuclear embryos was 0.6, 0, 32.3, and 52.4% for 0—96, 24—72, 48—48, and 96—0 hr at 37 and 39°C, respectively. Only 0—96 and 24—72 hr at 37 and 39°C were not different (P >0.10). Overall M/B development for two-cell embryos was 48.1, 78.1, and 98.0% for 0—72, 24—48, and 72—0 hr at 37 and 39°C, respectively. Percentage development at each time was different (P <.01) for each category. Additionally, the number of nuclei for morulae and blastocysts tended to be higher for embryos initiating culture at the two-cell stage compared to pronuclear embryos. The first cell cycle was most dramatically affected by a 2°C increase in incubator temperature. More advanced embryos can tolerate slight increases in incubator temperature more readily than pronuclear embryos. 相似文献
82.
研究了吡哌酸与四氯苯醌间的荷移反应.两者在硼砂缓冲溶液中形成1∶1的络合物,其λ_(max)为330.8nm,摩尔吸光系数ε=1.66×10~4,RSD为2.2%。用于测定制剂含量,结果与药典方法一致。 相似文献
83.
84.
目的 通过有限元分析,给出低温手术过程组织三维瞬态温度场;考察人体血液灌注和代谢产热对其影响,为优化设计冷刀提供参考。方法 充分考虑组织物性和内热源的非线性,使用基于有限容积法的大型商业有限元软件Ansys求解描述低温手术过程组织传热问题的经典生物传热方程。结果 考虑血液灌注和代谢产热后,组织局部降/复温速率降低、所经历的最低温度绝对数值减少;对应时刻,组织内局部温度梯度增加;相同手术方案下,组织内冻结区域减小,且冰球生长过程减慢。结论 人体血液灌注和代谢产热对低温手术过程影响显著,体外实验由于无法模拟血液灌注和代谢产热效果而对冷刀的优化设计作用有限,必须进行在体实验以优化冷刀及其相应的低温手术方案。 相似文献
85.
《Journal of labelled compounds & radiopharmaceuticals》2006,49(8):707-732
Results are reported on the regioselective C‐deuteriation of 2‐methyl‐tetralone using a series of D‐sources and tertiary amines as potential mediators. The results presented further aid the understanding of kinetic deuteriation of both ‘base‐containing’ and ‘base‐free’ enolates. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
86.
R. P. F. DULLAART W. J. SLUITER L. D. DIKKESCHEI† K. HOOGENBERG A. VAN TOL‡ 《European journal of clinical investigation》1994,24(3):188-194
Abstract. The mechanisms responsible for the decreased high density lipoprotein (HDL) cholesterol levels associated with obesity and insulin resistance are not well understood. Lecithin: cholesterol acyltransferase (LCAT) and cholesterol ester transfer protein (CETP) are key factors in the esterification of cholesterol in HDL and the subsequent transfer of cholesteryl ester towards apolipoprotein B-containing lipoproteins. Phospholipid transfer protein (PLTP) may be involved in the regulation of HDL particle size. We therefore measured the activities of LCAT, CETP and PLTP using exogenous substrate assays, as well as lipids, lipoproteins, insulin and C-peptide in fasting plasma from eight healthy obese men (body mass index >27 kg m-2) and 24 non-obese subjects. The obese men had lower levels of HDL cholesterol (P<0·05) and higher levels of plasma triglycerides (P<0·05), insulin (P<0·05) and C-peptide (P<0·01), as compared to the quartile of subjects with the lowest body mass index (BMI <22·4 kg m-2). CETP and PLTP activities were elevated in the obese men by 35% (P<0·01) and by 15% (P<0·05), respectively. LCAT activity was comparable among the quartiles. Linear regression analysis showed that CETP activity was positively correlated with body mass index (P<0·02), fasting blood glucose (P7lt;0·05) and plasma C-peptide (P<0·05). PLTP activity was positively related to body mass index (P<0·01), waist to hip circumference ratio (P<0·001), as well as to fasting blood glucose (P<0·05) and plasma C-peptide (P<0·05) It is concluded that the activities of CETP and PLTP are influenced by adiposity and possibly by insulin resistance. Elevated lipid transfer protein activities may provide a mechanism that contributes to alterations in HDL in insulin resistant states. 相似文献
87.
本文将小鼠宫颈癌腹水瘤u_(14)和经本教研室活体筛选出的倾向于肺转移的u_(14)A-P_(11)和倾向于淋巴转移的u_(14)-AL_(10)两亚株,用鸡胚绒毛尿囊膜接种的体内方法和与经胰酶不同处理的BALB/C小鼠腹膜组织共同培养的体外方法,对三种瘤细胞的侵袭特性进行研究。结果证明,三种瘤细胞体内转移倾向的差异与其对鸡胚绒毛尿囊膜基质和小鼠腹膜基质的侵袭能力成正相关,表明瘤细胞转移的器官倾向性与瘤细胞的侵袭力有一定的关系。 相似文献
88.
抗乙肝胎盘转移因子的特异免疫活性观察 总被引:8,自引:0,他引:8
本研究由HBVM-Ab阳性胎盘提取的抗乙肝胎盘转移因子(PSTF)的特异免疫活性证明,经注射,小鼠外周血淋巴细胞明显增多,胸腺重量和指数亦显著增加,酯酶染色证明75%属T-样淋巴细胞,25%属Thy-样淋巴细胞,人白细胞或小鼠白细胞体外转移PSTF特异免疫活性、或豚鼠和小鼠体内转移PSTF特异免疫活性,再取其白细胞作白细胞粘附抑制、白细胞移动抑制、特异淋巴细胞转化、小鼠足掌注射和诱生γ-干扰素试验 相似文献
89.
A critical assessment of allergen component-based in vitro diagnosis in cherry allergy across Europe
A. Reuter J. Lidholm K. Andersson J. Östling M. Lundberg S. Scheurer E. Enrique A. Cistero-Bahima M. San Miguel-Moncin B. K. Ballmer-Weber Stefan Vieths 《Clinical and experimental allergy》2006,36(6):815-823
BACKGROUND: Food allergy to cherry occurs throughout Europe, typically with restricted oral reactions in the central and northern parts but with frequent systemic reactions in the Mediterranean region. Previous studies have demonstrated insufficient sensitivity of commercially available cherry extract reagents in the diagnosis of cherry allergy. OBJECTIVE: To assess the diagnostic performance of specific IgE tests based on recombinant cherry allergens in comparison with an extract-based assay and to skin prick test (SPT). A secondary objective was to analyse the frequency of systemic reactions in cherry-allergic subjects across Europe, including the largest population of LTP-sensitized subjects from central Europe studied to date. METHODS: A total of 186 subjects from central Europe and Spain were studied. Serum IgE was analysed with ImmunoCAP tests carrying rPru av 1, 3 and 4, combined and separately, and cherry extract. RESULTS: Among the central European cherry allergics, the mix of rPru av 1, 3 and 4 had a sensitivity of 95%, compared with 65% for cherry extract, and the IgE binding capacity of the recombinant mix was considerably higher. The sensitivity of the two tests was more comparable in the Spanish population, 95% and 86%, respectively. The recombinant allergen ImmunoCAP equalled SPT in terms of sensitivity and specificity. Consistent with previous reports, major geographic differences in sensitization pattern and prevalence of systemic reactions were found. A significantly higher rate of systemic reactions was found in Spanish patients sensitized to Pru av 3 whereas German patients sensitized to LTP only had oral allergy syndrome. CONCLUSIONS: The recombinant cherry allergen ImmunoCAP is a highly sensitive diagnostic tool, clearly superior to any diagnostic method based on cherry extract. Three cherry allergens are sufficient for detecting sensitization in 95% of cherry-allergic subjects. Systemic reactions are common in LTP-sensitized individuals but seem to require at least one additional causative factor. 相似文献
90.