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101.
Yasueda Saito Sakaguchi Ide Saito Taniguchi Akiyama & Inouye 《Clinical and experimental allergy》2000,30(4):546-550
BACKGROUND: Not only Cryptomeria japonica (Japanese cedar) pollen but also that of Chamaecyparis obtusa (Japanese cypress) induces the allergic symptoms of Japanese cedar pollinosis. However, allergens from C. obtusa pollen have not been as well characterized as those from C. japonica pollen. OBJECTIVE: We sought to identify and characterize a homologue of the second major allergen of C. japonica pollen, Cry j 2, from the pollen of C. obtusa. METHODS: An allergen homologous to Cry j 2 was identified in C. obtusa pollen extract by immunoblot analysis, probed with anti-Cry j 2 monoclonal antibodies and purified by a series of column chromatographic steps. RESULTS: The allergen isolated from the extract showed a slightly diffuse band of 45 kDa and closely spaced double-bands of 42 and 45 kDa on SDS-PAGE, under reducing and non-reducing conditions, respectively; the bands were approximately 5-7 kDa larger than those of Cry j 2. In 24 of 30 residues, the N-terminal amino acid sequence of the allergen was identical with corresponding sequence in Cry j 2. Most patients with pollinosis who were IgE antibody-positive to Cry j 2 were shown to be IgE antibody-positive to this allergen, and the IgE antibody levels to both allergens were highly correlated. CONCLUSION: The results indicate that the allergen isolated from C. obtusa pollen in this study is a homologue of Cry j 2. The allergen was designated as Cha o 2 according to the WHO/IUIS Allergen Nomenclature Subcommittee recommendation. 相似文献
102.
C. Egger M. Focke A. J. Bircher K. Scherer N. Mothes‐Luksch F. Horak R. Valenta 《Clinical and experimental allergy》2008,38(10):1688-1696
Background Beech and oak pollen are potential allergen sources with a world‐wide distribution. Objective We aimed to characterize the allergen profile of beech and oak pollen and to study cross‐reactivities with birch and grass pollen allergens. Methods Sera from tree pollen‐allergic patients with evidence for beech and oak pollen sensitization from Basel, Switzerland, (n=23) and sera from birch pollen‐allergic patients from Vienna, Austria, (n=26) were compared in immunoblot experiments for IgE reactivity to birch (Betula pendula syn. verrucosa), beech (Fagus sylvatica) and oak (Quercus alba) pollen allergens. Subsequently, beech and oak pollen allergens were characterized by IgE inhibition experiments with purified recombinant and natural allergens and with allergen‐specific antibody probes. Birch‐, beech‐ and oak pollen‐specific IgE levels were determined by ELISA. Results Beech and oak pollen contain allergens that cross‐react with the birch pollen allergens Bet v 1, Bet v 2 and Bet v 4 and with the berberine bridge enzyme‐like allergen Phl p 4 from timothy grass pollen. Sera from Swiss and Austrian patients exhibited similar IgE reactivity profiles to birch, beech and oak pollen extracts. IgE levels to beech and oak pollen allergens were lower than those to birch pollen allergens. Conclusion IgE reactivity to beech pollen is mainly due to cross‐reactivity with birch pollen allergens, and a Phl p 4‐like molecule represented another predominant IgE‐reactive structure in oak pollen. The characterization of beech and oak pollen allergens and their cross‐reactivity is important for the diagnosis and treatment of beech and oak pollen allergy. 相似文献
103.
104.
Twenty-four children with rhinoconjunctivitis due to birch pollinosis were treated in a double-blind manner with enteric-coated capsules containing either a high dose of a birch pollen preparation (n = 11) or placebo (n = 13). The permeability of the small intestine was analysed at three different occasions with a mixture of differently sized polyethyleneglycols (PEG 400 and PEG 1000), before the start of oral immunotherapy (OIT), at the moment of maximum allergen dose, and after 3 months of therapy which was at the beginning of the pollen season. The actively treated children did not significantly change their permeability characteristics as determined from PEG recovery in the urine. By contrast, in the control group of patients the recovery of larger PEG molecules was decreased after 3 months of therapy, possibly due to the commencing pollen season. In addition, small bowel biopsies were taken at the time of maximum allergen dose from two children openly treated with OIT. Both specimens were normal. 相似文献
105.
BACKGROUND: Cyn d 1, the major allergen of Bermuda grass pollen, consists of a number of isoforms. OBJECTIVE: To examine the extent of sequence variation of Cyn d 1 isoforms at the molecular level. METHODS: A Bermuda grass pollen lambdaZAP II cDNA expression library was immunoscreened with anti-Cyn d 1 monoclonal antibodies. The reactive clones were isolated, subcloned into Escherichia coli, and sequenced. Some of them were expressed in the yeast Pichia pastoris to obtain recombinant Cyn d 1 proteins. RESULTS: Ten cDNA clones were obtained, all these clones encode the full length of Cyn d 1 protein. Their deduced mature proteins can be grouped into: the long ones with 246 amino acids, and the short ones with 244 amino acids. The last two amino acids (AG) of the long Cyn d 1 are deleted in the short Cyn d 1. The remaining amino acid sequences share more than 98% identity; a total of nine amino acid variations were observed. Two recombinant Cyn d 1 proteins (rCyn d 3-2 and rCyn d 5-4) with three amino acid substitutions showed differential IgE-binding profiles. CONCLUSION: The present study extended our understanding of the primary structure of isoforms of Cyn d 1. 相似文献
106.
M. Ceglecka J. Wjcicki B. Gonet A. Put W. Ku
na-Grygiel L. Samochowiec 《Phytotherapy research : PTR》1991,5(6):245-249
Male Wistar rats were divided into four equal groups: (i) controls, (ii) animals exposed to organic solvents, (iii) animals exposed to organic solvents and receiving pollen extracts, (iv) rats given pollen extracts. The experiment lasted 3 months. The protective effect of pollen extracts against changes evoked by organic solvents was demonstrated. Pollen extracts inhibited or counteracted the elevation of aminotransferases and alkaline phosphatase activity and lipid and carbohydrate metabolism disturbances in the liver. The ascorbate system was improved. It is concluded, that pollen extracts are able to protect the liver against changes evoked by environmental influences. 相似文献
107.
108.
109.
Diagnostic relevance of IgE sensitization profiles to eight recombinant Phleum pratense molecules 下载免费PDF全文
F. Cipriani C. Mastrorilli S. Tripodi G. Ricci S. Perna V. Panetta R. Asero A. Dondi A. Bianchi N. Maiello M. Miraglia del Giudice T. Frediani F. Macrì S. Lucarelli I. Dello Iacono M. F. Patria E. Varin D. Peroni L. Chini V. Moschese R. Bernardini G. Pingitore U. Pelosi M. Tosca F. Paravati I. Sfika A. Di Rienzo Businco C. Povesi Dascola P. Comberiati S. Frediani C. Lambiase M. C. Verga D. Faggian M. Plebani M. Calvani C. Caffarelli P. M. Matricardi the Italian Pediatric Allergy Network 《Allergy》2018,73(3):673-682
110.
A randomized,double‐blind,placebo‐controlled,dose‐finding trial with Lolium perenne peptide immunotherapy 下载免费PDF全文
R. Mösges E. M. Kasche E. Raskopf J. Singh L. Sohlich A. Astvatsatourov K. Shah‐Hosseini S. Pirotton L. Haazen S. R. Durham T. Legon G. Zadoyan M. H. Shamji 《Allergy》2018,73(4):896-904