Transmembrane G protein-coupled receptor 30 gene polymorphisms and uterine adenomyosis in Korean women

Abstract

To compare the genetic distributions of 14G protein-coupled receptor 30 (GPR30) single-nucleotide polymorphisms (SNPs) between women with and without uterine adenomyosis. The study population comprised 69 Korean women. Uterine tissues from the adenomyosis and non-adenomyosis groups were used for DNA extraction. Pre-designed PCR/Sanger or Sequencing Primer and TaqMan^® SNP Genotyping Assays were used for the SNP genotyping of the GPR30 gene. Immunohistochemical staining was performed to confirm the GPR30 expression. Differences in genotype and allele frequencies between the two groups were calculated using Fisher’s exact test. The rs3802141 CT genotype was more common in the control group (p?=?.02), and the rs4266553?CC genotype was more common in the adenomyosis group (p?=?.02). The C allele of the SNP rs4266553 was more common in the adenomyosis group (p?=?.02). GPR30 expression was confirmed in 69 individuals in both groups. GPR30 gene polymorphism is presumed to affect the risk of adenomyosis with limited sample size. Further large-scale study is needed to explain the genetic influence of GPR30 gene polymorphism.     

子宫腺肌病100例超声诊断体会

目的：探讨超声诊断子宫腺肌病的临床价值。方法：选择我院收治的均由术后病理证实的子宫腺肌病病例100例,对其二维图像特征及彩色多普勒血流特征进行回顾性分析。结果：在本组100例病例中,超声诊断与病理结果相符98例,符合率为98.0%,局限性子宫腺肌病误诊为子宫肌瘤2例;合并卵巢巧克力囊肿72例,漏诊8例,诊断率为90.0%;合并子宫肌瘤38例均符合。结论：超声诊断子宫腺肌病准确率较高,可为临床诊断及治疗提供重要的参考依据。     

子宫腺肌病动物模型建立及应用进展

子宫腺肌病是指子宫内膜在子宫肌层内的良性侵入和扩散,是妇科常见病。多发生于四十岁以上的经产妇,以进行性痛经、月经过多、贫血为主要临床表现,目前发病机制不清,缺乏有效的治疗手段。动物模型的应用对该病的研究具有重要作用。许多种类的动物可自发子宫腺肌病,小鼠的自发率相对较高,通过药物诱导或手术诱导的方法均可使其发病率明显增高,具体机制尚不清,就有关子宫腺肌病动物模型的类型及其应用进行的相关研究做文献综述。     

曼月乐联合散结镇痛胶囊对子宫腺肌病患者子宫体积、痛经及月经的影响

目的:探讨曼月乐联合散结镇痛胶囊对子宫腺肌病患者子宫体积、痛经及月经的影响。方法:选取2015年11月至2017年2月中山市火炬开发区医院收治的子宫腺肌病患者78例,随机分为观察组与对照组,每组39例。对照组患者于宫腔处放置曼月乐,观察组在对照组的基础上口服散结镇痛胶囊,2组治疗6个月。比较2组患者临床疗效;统计并比较治疗前后2组患者子宫体积、痛经及月经情况;记录2组不良反应发生情况。结果:治疗6个月后,观察组与对照组的总有效率分别为87.18%、66.67%,观察组总有效率显著高于对照组(P0.05);与治疗前比较,治疗1、3、6个月后2组患者子宫体积均显著缩小,视觉模拟评分法(VAS)及月经量评分(PBAC)评分及血清癌抗原125(CA125)水平VAS、PBAC评分及血清CA125水平均显著下降(P0.05),且治疗后各时间点观察组患者子宫体积均显著小于对照组,观察组VAS、PBAC评分及血清CA125水平均显著低于对照组(P0.05);观察组不良反应总发生率(15.38%)显著低于对照组的(30.77%)(P0.05)。结论:曼月乐联合散结镇痛胶囊治疗子宫腺肌可显著缩小患者子宫体积、改善痛经及月经量,且安全性较好。     

Report of two cases of Accessory Cavitated Uterine Mass (ACUM): Diagnostic challenge for MRI

Cystic adenomyosis is an unusual form of adenomyosis, characterized by a well-circumscribed cavitated endometrial gland and stroma, ≥ 1 cm in diameter, located within the myometrium. Few cases have been reported in the gynecological literature, with confusing naming such as: juvenile cystic adenomyosis, cystic myometrial lesions, cystic adenomyoma or juvenile adenomyotic cysts. The current preferred terminology is accessory cavitated uterine mass /or malformation (ACUM). We report here the cases of two 17 and 18 -year-old nulliparous women, who complained of severe dysmenorrhea early after the onset of menarche, with none or partial efficiency of medical treatment. MRI findings, with a follow-up in one case and surgical treatment in both cases, are described with an emphasis on physiopathology. The typical MR appearance is a large well-circumscribed round mass within the external myometrium, composed by an inner cystic hemorrhagic layer surrounded by a thick fibrous crown. The first-line treatment is laparoscopic surgery with mass resection. This typical MRI pattern must be a part of the knowledge of the radiologists.     

Mullerian duct failure: An unusual case with adenomyosis managed with total hysterectomy

A 45-year-old woman admitted to our hospital complaining of perimenopausal uterine bleeding not responding to medical treatment. Ultrasound evaluation revealed unicornuate uterus with adenomyosis and it was so difficult to see the distant small left rudimentary horn on ultrasound. The patient underwent laparotomy with total hysterectomy for both horns and was sent to pathologist that indicated adenomyosis and non-communicating non-cavitated left rudimentary horn.