The effect of calcium dobesilate on venous function following saphenectomy in coronary artery bypass grafting

BACKGROUND: The aim of this study was to determine whether prophylactic use of calcium dobesilate (CD) can improve venous function after saphenous vein harvest in coronary artery bypass graft (CABG). MATERIALS AND METHODS: A total of 100 patients who underwent elective CABG were divided into four equal groups. In Group A, the greater saphenous vein (GSV) was harvested below the knee and, in Group B, through the knee till the groin. These patients remained untreated. Group C received CD in a dosage of 1500 mg po daily after the GSV was harvested below the knee. Group D received same dosage of CD after the GSV was harvested through the knee till the groin. Venous function of ipsilateral leg was evaluated clinically and by Doppler ultrasonography in the postoperative first week and second month. RESULTS: Clinical findings of venous insufficiency were observed with a similar rate between groups at both early and late periods. In Groups A and B, after 2 months, flow velocities decreased and reflux periods increased significantly. In groups C and D, treatment with CD for 2 months after saphenectomy resulted in a significant increase in flow velocities and a significant decrease in reflux periods. Patients in Groups B and D have significantly more impaired venous functions. CONCLUSION: Saphenectomy results in ipsilateral leg venous dysfunction, which seems to be unrelated to leg swelling and to be more prominent in patients with high-level saphenectomy. In addition, to be careful about the restriction of the saphenectomy procedure into the most appropriate level, prophylactic use of CD can prevent this deterioration when it was added to varice socks.     

钙剂加维生素D治疗对糖尿病患者钙代谢的影响

本文报道NIDDM患者在常规治疗的基础上加用钙剂和维生素D治疗后的钙代谢变化。糖尿病患者单纯用常规治疗控制血糖后,负钙平衡不能完全纠正,而加用钙剂和维生素D治疗后,负钙平衡能完全纠正,并且高于正常对照组平衡值(P<0.01)。说明钙剂加维生素D治疗对糖尿病性骨质疏松是有益的。     

Parvalbumin expression reveals a vibrissa-related pattern in rabbit SI cortex

The expression of parvalbumin-like immunoreactivity (PV-LIR) was examined in the mystacial representation within the primary somatosensory cortex (SI) of postnatal day 21 and adult rabbits. PV-LIR was expressed in a prominent vibrissa-like array of patches in layer IV despite the fact that barrels were indistinct in the cytoarchitecture. Each patch consisted of dense terminal-like PV-LIR and a preferential concentration of intensely labeled stellate neurons. Layer V contained scattered small and large intensely labeled basket cells. Layer Vb had a distinct layer of lightly labeled large pyramidal cells that received labeled basket cell terminations. Upper layer VI also contained patches of terminal-like PV-LIR that were in register with the overlying vibrissae pattern. These patches also contained a preferential distribution of labeled non-pyramidal cells as well as modified pyramidal cells. These results suggest that PV-LIR in rabbits delineates cortical modules composed of thalamorcotical afferents and inhibitory local circuits in the absence of a distinct barrel cytoarchitecure. In contrast, prior studies of rat SI cortex have revealed a distinct barrel cytoarchitecture but a uniform distribution of PV-LIR. The differences in PV-LIR between rodents and lagomorphs within the vibrissae representation in SI may be related to species differences in thalamic and local cortical circuits devoted to the whisker sense.     

维拉帕米抑制培养细胞DNA、RNA和蛋白质合成的作用

为探讨钙拮抗剂对细胞大分子物质的代谢影响,本文观察了维拉帕米对人成纤维细胞(2BS)和猴内皮细胞(RF/6A)DNA,RNA和蛋白质合成的作用.维拉帕米抑制2BS细胞DNA和RNA合成的作用为浓度依赖性,ID_(50)分别为12.3和22μg/ml.抑制RF细胞DNA和蛋白质合成的ID_(50)分别为34.4和49.6μg/ml.     

腹主动脉灌注丙泊酚减轻脊髓缺血-再灌注损伤与抗氧化和减轻钙超载的关系

目的 本研究拟探讨腹主动脉灌注丙泊酚减轻脊髓缺血-再灌注损伤是否与其抗氧化和减轻钙超载相关.方法 新西兰大耳白兔60只,随机均分为生理盐水(NS)组、10%脂肪乳(P0)组、丙泊酚30mg/kg(P30)组、40mg/kg(P40)组、50mg/kg(P50)组和60mg/kg(P60)组.全麻后开腹阻断肾动脉远端腹主动脉及双侧髂总动脉30min,并从阻断即刻开始,经股动脉预置导管向腹主动脉分别灌注生理盐水、10%脂肪乳及不同剂量的丙泊酚溶液至开放停止.再灌注48h,取L5脊髓节段,检测脊髓组织的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和脊髓前角神经细胞内Ca2 荧光强度.结果 与NS和P0组比较,P30、P40、P50和P60组的SOD活性显著升高(P<0.05),MDA含量和Ca2 荧光强度显著降低(P<0.05).SOD活性:P50组最高(P<0.05),P40、P60组高于P30组(P<0.05).MDA含量:P50组最低(P<0.05),P60组低于P30、P40组(P<0.05).Ca2 荧光强度:P60组低于P50组,P50组低于P30、P40组(P<0.05).结论 丙泊酚可剂量依赖性地保护脊髓组织SOD活性、降低MDA含量和脊髓前角神经细胞内Ca2 荧光强度,提示该保护作用与其抗氧化和减轻神经细胞钙超载有关.     

脱钙骨基质与左旋聚乳酸/聚磷酸钙纤维对骨髓间充质干细胞黏附作用的比较

目的探讨左旋聚乳酸／聚磷酸钙纤维（PLLA／CPPf）与同种异体的脱钙骨基质（DBM）对BMSCs黏附作用的差异，为构建组织工程骨提供理想的支架材料。方法用扫描电镜观察两种支架材料的孔径；通过测吸水率比较其亲水性。将普通培养瓶培养的第3代BMSCs分别成骨诱导培养1周后，在体外分别与PLLA／CPPf和同种异体的DBM复合构建组织工程骨，于构建后10h计算细胞黏附率，并用扫描电镜观察比较两种支架材料对BMSCs黏附作用的差异。构建后每天在相差显微镜下观察BMSCs在两种支架材料上的生长情况，构建后第10天再行扫描电镜观察BMSCs在两种支架材料上的生长情况。结果DBM的孔径明显较PLLA／CPPf的大，孔隙率高，体外构建后10hDBM组的细胞黏附率为（91．3±8．2）％，PLLA/CPPf组为（82．2±7．7）％，两者之间差异具有统计学意义（P〈0．05）。DBM组于术后第3天网孔间出现细胞外基质，且随着时间的推移，基质变得稠密，而PLLA／CPPf的细胞外基质鲜见；第10天的扫描电镜也证实DBM组的细胞在支架材料上数量多，且细胞外的基质分泌较多。结论DBM较PLLA／CPPf更适于BMSCs的黏附生长，是理想的组织工程支架材料。     

β淀粉样肽25-35对原代培养的海马神经元电压门控钙通道电流的影响

目的 探讨β淀粉样肽25-35(Aβ25-35)对海马神经元电压门控的钙通道电流(VGCC)的作用.方法 应用全细胞膜片钳技术记录海马神经元上的VGCC,通过设定不同的钳制电压分别记录高电压激活的钙电流(HVA-ICa)和低电压激活的钙电流(LVA-ICa),并观察Aβ25-35对其的影响.结果 分别对原代培养的海马神经元急性胞外给予2μmol/L和10 μmol/L的凝聚态Aβ25-35,在最大激活电压下加药后ICa幅度分别是加药前的99.80%±0.02%及100.00%±1.58%,差异没有统计学意义.10 μmol/L凝聚态Aβ25-35预孵育24 h可增大ICa,对照组(未给Aβ25-35)为(24.49±4.35)pA/pF,Aβ25-35组(预孵育24 h)为(46.59±7.15)pA/pF,两组差异有统计学意义(P＜0.05);但Aβ25-35组的膜电容[(14.34±1.74)pF]与对照组[(14.44±0.97)pF]相比差异没有统计学意义.结论 急性给予凝聚态Aβ25-35对VGCC没有影响,24 h预孵育凝聚态Aβ25-35增大了VGCC,而膜电容没有改变,提示凝聚态Aβ25-35可通过对细胞膜上钙通道进行分子调控以增大VGCC.     

碳酸锂合并丙戊酸钠预防双相障碍复发的研究

目的比较碳酸锂合并丙戊酸钠与单用碳酸锂对预防双相障碍复发的疗效。方法对82例临床康复的双相障碍患者随机分为碳酸锂合并丙戊酸钠组及单用碳酸锂组,合并用药组44例,单用碳酸锂组38例,随访观察2年。结果合并用药组有效率为88.6%,单用碳酸锂组有效率为68.4%,两组比较差异有显著性(P<0.05)。结论碳酸锂合并丙戊酸钠对双相障碍有较好的预防复发作用。     

氯胺酮对荷包牡丹碱诱导PC12细胞内Ca2+浓度波动方式的影响

目的研究氯胺酮对荷包牡丹碱诱导PCI2细胞内Ca^2＋浓度波动方式的影响。方法使用含25ng／LNGF的DMED培养基在多聚赖氨酸包被的培养皿中培养PCl2细胞；与终浓度10gmol／L的Ca^2＋指示剂Fluo-3 AM ester共孵育30min洗涤后，加入终浓度50gmol／L荷包牡丹碱；在激光共聚焦显微镜选定多个细胞分别测定荧光强度的变化；随后加入氯胺酮，记录细胞荧光强度的改变。在试验结束前依次加入Triton X-100和EGTA分别记录单个细胞最大荧光强度（Fmax）和最小荧光强度（Fmin），以计算细胞内Ca^2＋的相对强度。结果氯胺酮不改变荷包牡丹碱诱导PCl2细胞内Ca^2＋浓度波动的基线，但抑制细胞内Ca^2＋浓度升高的幅度（P〈0．05），缩短相邻波峰间的时间间隙（P〈0．05）。结论氯胺酮不仅改变荷包牡丹碱诱导PCl2细胞内Ca^2＋浓度升高的幅度，而且改变Ca^2＋浓度波动的周期。