XIAP基因在慢性髓系白血病中的表达及临床意义

本研究旨在检测慢性髓系白血病(CML)患者XIAP基因mRNA的表达情况,以探讨其在CML病情进展及预后中的意义。应用染色体R显带技术及实时PCR技术对71例CML患者和10名健康者进行染色体核型分析及XIAP mRNA检测。结果表明,XIAP mRNA在CML的加速期和急变期的平均表达水平明显高于慢性期(p<0.01)。XIAP基因在不同核型组的平均表达水平无显著性差异。结论:CML患者加速期和急变期XIAP基因表达明显增高,XIAP基因与CML的病情进展密切相关。     

Differential binding of [3H]MK-801 to brain regions and spinal cord of mice treated chronically with morphine

• 1.1. The effects of morphine tolerance and abstinence on the binding of [³H]MK-801, a noncompetitive antagonist of the N-methyl-D-aspartate (NMDA) receptors were determined in brain regions and spinal cord of the mouse.
• 2.2. Male Swiss-Webster mice were rendered tolerant to and physically dependent on morphine by subcutaneous implantation of a pellet containing 75 mg of morphine base for 3 days. Placebo pellet-implanted mice served as controls. In tolerant (nonabstinent) mice, the pellets were left intact at the time of sacrificing whereas, in the abstinent mice, the pellets were removed 6 hr prior to sacrificing.
• 3.3. The binding of [³H]MK-801 to membranes prepared from spinal cord and brain regions (cortex, pons-medulla, hypothalamus, hippocampus, amygdala, striatum, and midbrain) was determined by using a 5 nM concentration of the ligand in the presence of 30 μM glycine and 50 μM of glutamate.
• 4.4. In nonabstinent morphine-tolerant mice, the binding of [³H]MK-801 was decreased in pons-medulla and hypothalamus, but was increased in the spinal cord in comparison to that in placebo controls. The reduction in binding in pons-medulla was due to a decrease in the B_max value; the K_d value remained unchanged. The binding of [³H]MK-801 was increased in the hippocampus of morphine-abstinent mice.
• 5.5. These studies demonstrate that NMDA receptors of brain regions and spinal cord are differentially affected in morphine-tolerant and abstinent mice.