全文获取类型
收费全文 | 6298篇 |
免费 | 534篇 |
国内免费 | 107篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 95篇 |
妇产科学 | 36篇 |
基础医学 | 562篇 |
口腔科学 | 20篇 |
临床医学 | 450篇 |
内科学 | 3688篇 |
皮肤病学 | 18篇 |
神经病学 | 103篇 |
特种医学 | 95篇 |
外科学 | 662篇 |
综合类 | 169篇 |
现状与发展 | 2篇 |
一般理论 | 1篇 |
预防医学 | 297篇 |
眼科学 | 9篇 |
药学 | 357篇 |
4篇 | |
中国医学 | 71篇 |
肿瘤学 | 297篇 |
出版年
2024年 | 10篇 |
2023年 | 417篇 |
2022年 | 566篇 |
2021年 | 649篇 |
2020年 | 593篇 |
2019年 | 345篇 |
2018年 | 300篇 |
2017年 | 319篇 |
2016年 | 295篇 |
2015年 | 306篇 |
2014年 | 507篇 |
2013年 | 377篇 |
2012年 | 297篇 |
2011年 | 227篇 |
2010年 | 263篇 |
2009年 | 283篇 |
2008年 | 133篇 |
2007年 | 127篇 |
2006年 | 124篇 |
2005年 | 105篇 |
2004年 | 70篇 |
2003年 | 57篇 |
2002年 | 75篇 |
2001年 | 60篇 |
2000年 | 58篇 |
1999年 | 62篇 |
1998年 | 52篇 |
1997年 | 34篇 |
1996年 | 43篇 |
1995年 | 49篇 |
1994年 | 26篇 |
1993年 | 25篇 |
1992年 | 21篇 |
1991年 | 11篇 |
1990年 | 5篇 |
1989年 | 13篇 |
1988年 | 14篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有6939条查询结果,搜索用时 15 毫秒
81.
82.
83.
Koushan Sineh Sepehr Mohsen Saeidi Majid Mossahebi-Mohammadi Meghdad Abdollahpour-Alitappeh Seyed Mahmoud Hashemi 《Journal of immunoassay & immunochemistry》2018,39(2):207-217
Background: Mesenchymal stem cells (MSCs) were isolated from various sources, including various types of tumors. However choosing an appropriate isolation method is an important step in obtaining cells with optimal quality and yield in companion with economical considerations. The purpose of this study was to isolate more pure MSCs from human breast tumor tissue by a modified explant culture method.Methods and Materials: The tumor tissues (n = 8) were cut into 1 to 3-mm cube-like pieces (explant). Each explant was placed in a well of 24-well format plates, cultured in Dulbecco’s Modified Eagle’s medium (DMEM), and maintained at 37°C with 5% humidified incubator. Morphological phenotypes of the cells were surveyed by an inverted microscope and wells with rather homogenous fibroblast-like morphology cell were considered as positive and selected for more expansion and characterization.Results: A total of 185 wells, 63.7% of wells were positive that were chosen for expansion. Flowcytometry analysis demonstrated that isolated cells were positive for CD73, CD44, CD29, CD105, and CD90 but negative for CD11b, CD45, CD34, and HLA?DR. In addition, cells possessed the capability of multipotential differentiation into osteoblasts and adipocytes. 相似文献
84.
Vidthiya Menon Rebecca Davis Nick Shackel Bjorn A. Espedido Alicia G. Beukers Slade O. Jensen Sebastiaan J. van Hal 《Diagnostic microbiology and infectious disease》2018,90(2):120-122
Daptomycin β-Lactam combination therapy offers “protection” against daptomycin non-susceptibility (DNS) development in Enterococcus faecium. We report failure of this strategy and the importance of source control. Mutations were detected in the LiaF and cls genes in DNS isolates. A single DNS isolate contained an unrecognized mutation, which requires confirmation. 相似文献
85.
86.
《Genetics in medicine》2017,19(2):160-168
PurposeThe cytosolic enzyme N-glycanase 1, encoded by NGLY1, catalyzes cleavage of the β-aspartyl glycosylamine bond of N-linked glycoproteins, releasing intact N-glycans from proteins bound for degradation. In this study, we describe the clinical spectrum of NGLY1 deficiency (NGLY1-CDDG).MethodsProspective natural history protocol.ResultsIn 12 individuals ages 2 to 21 years with confirmed, biallelic, pathogenic NGLY1 mutations, we identified previously unreported clinical features, including optic atrophy and retinal pigmentary changes/cone dystrophy, delayed bone age, joint hypermobility, and lower than predicted resting energy expenditure. Novel laboratory findings include low cerebral spinal fluid (CSF) total protein and albumin and unusually high antibody titers toward rubella and/or rubeola following vaccination. We also confirmed and further quantified previously reported findings noting that decreased tear production, transient transaminitis, small feet, a complex hyperkinetic movement disorder, and varying degrees of global developmental delay with relatively preserved socialization are the most consistent features.ConclusionOur prospective phenotyping expands the clinical spectrum of NGLY1-CDDG, offers prognostic information, and provides baseline data for evaluating therapeutic interventions. 相似文献
87.
88.
89.
《Best Practice & Research: Clinical Gastroenterology》2014,28(5):783-793
When hepatocellular carcinoma presents with symptoms cure is seldom possible and death usually follows within months. However, it is possible to detect HCC early, at which stage it is curable. This requires a surveillance program. The components of such a program include: identification of the at risk population, provision of appropriate surveillance tests, and an appropriate method of determining whether the abnormalities found on screening are cancer or not. Surveillance for liver cancer meets all these criteria. Unfortunately high quality evidence showing benefit of liver cancer surveillance is lacking, but lesser quality evidence is plentiful, including several cost efficacy analyses that all show that surveillance does decrease mortality. Therefore all the continental liver disease societies and all national liver disease societies have recommended that surveillance should be undertaken. 相似文献
90.