正交试验法优选苦参的半仿生法提取工艺条件

目的:通过正交试验优选苦参的半仿生法提取最佳工艺。方法:以氧化苦参碱含量为指标,在提取次数、提取工艺、样品浓缩检测等其他条件相同时,考虑第1煎提取液pH值、第2煎提取液pH值、提取温度、每煎提取时间,选用L9(34)正交表安排实验,确定最佳提取工艺。结果:最佳提取工艺为第1煎选用pH=2.0的酸性提取液,第2煎选用pH=9.0的碱性提取液,提取温度控制为60℃,每煎提取时间为2.0h。结论:优选出的半仿生法提取苦参最佳工艺稳定可靠,可行。     

健脾生血颗粒治疗肿瘤化疗相关性贫血临床观察

目的:观察健脾生血颗粒治疗肿瘤化疗相关性贫血的临床疗效及作用机制。方法:将100例符合肿瘤化疗相关性贫血诊断的患者,按随机数字表法随机分为2组,每组50例。2组患者均给予重组人红细胞生成素皮下注射10 000 IU/次,每周3次的基础治疗。治疗组给予健脾生血颗粒口服补铁,15 g/次,3次/d(每天补充铁元素180 mg);对照组给予硫酸亚铁缓释片口服补铁,0.45 g/次,2次/d(每天补充铁元素180 mg)。2组连续治疗30 d后观察中医证候评分及RBC、Hb、SF、KPS评分等指标变化,并评价有效率与不良事件发生情况。结果:2组患者治疗前各项指标比较无统计学意义(P0.05),具有可比性。治疗组患者均按医嘱完成治疗,对照组5例患者因为不能耐受消化道不良反应,于治疗1周内退出研究,2组脱落率有统计学意义(P0.05)。治疗治疗组临床总有效率明显高于对照组(P0.05);2组治疗后中医证候评分、RBC、Hb、SF、KPS等指标均有显著改善(P0.05),且治疗组显著优于对照组(P0.05)。治疗组不良事件发生率显著少于对照组(P0.05)。结论:健脾生血颗粒治疗肿瘤化疗相关性贫血疗效显著,安全性、依从性良好,值得临床推广应用。     

Protective immunity of a Pichia pastoris expressed recombinant iridovirus major capsid protein in the Chinese giant salamander,Andrias davidianus

The major capsid protein (MCP) is the main immunogenic protein of iridoviruses, that has been widely used as an immunogen in vaccination trials. In this study, the codon-optimized giant salamander iridovirus (GSIV) MCP gene (O-MCP) was synthesized and cloned into a pPICZα B vector for secretory expression in the methylotrophic yeast Pichia pastoris after methanol induction. The expression of the O-MCP protein was detected by the Bradford protein assay, SDS-PAGE, Western blotting and electron microscopy. The Bradford protein assay indicated that the concentration of the O-MCP expressed was about 40 μg/ml in culture supernatants. SDS-PAGE analysis revealed that the O-MCP had a molecular weight of about 66 kDa and reacted with a His-specific MAb that was confirmed by Western blotting. Electron microscopy observations revealed that the purified O-MCP could self-assemble into virus-like particles. Healthy giant salamanders were vaccinated by intramuscular injection with the O-MCP antigen at a dose of 20 μg/individual. The numbers of erythrocytes and leukocytes in the peripheral blood of immunized Chinese giant salamanders increased significantly at day 3 and reached a peak at day 5 post-immunization. Meanwhile, the differential leukocyte counts of monocytes and neutrophils increased significantly at day 5 post-immunization compared to that of the control group. The percentage of lymphocytes was 71.33 ± 3.57% at day 21 post-immunization. The neutralization assay showed that the serum neutralizing antibody titer reached 321 at day 21 post-immunization. The GSIV challenge test revealed that the relative percent survival of Chinese giant salamanders vaccinated with O-MCP was 78%. These results indicated that the O-MCP antigen expressed by the Pichia pastoris system elicited significant immune response in the Chinese giant salamander against GSIV and might represent a potential yeast-derived vaccine candidate that could be used for the control of disease caused by the giant salamander iridovirus.     

ALK基因荧光原位杂交分离探针制备及临床检测分析

目的:建立ALK分离荧光原位杂交探针（FISH）检测ALK基因重排,并对临床样本进行检测。方法:制备ALK基因分离探针,使用TFG-ALK和EML4-ALK基因重排样本验证探针,同时检测临床非小细胞肺癌样本。结果:采用本研究制备的ALK分离探针能准确检测出经病理确诊的TFG-ALK和EML4-ALK基因重排。结论:ALK分离探针荧光信号清晰,背景干扰较少,能够准确的检测多种形式ALK基因重排。     

不同介质中利培酮口溶膜剂溶出曲线相似性比较

摘 要 目的：考察不同溶出介质对利培酮口溶膜剂溶出度的影响,为其制剂质量判断提供依据。方法: 分别考察自制利培酮口溶膜剂和参比制剂在水、0.1 mol·L^-1 盐酸溶液、pH 4.5醋酸 醋酸钠缓冲液、pH 6.8磷酸盐缓冲液4种溶出介质中的体外溶出行为,以HPLC法进行测定,并采用溶出相似（f2）因子法评价自制制剂和参比制剂溶出曲线的相似性。结果: 在4种介质中,自制制剂与参比制剂的f2因子均大于50。结论: 自制制剂与参比制剂在4种介质中的溶出行为相似,表明该制剂的处方工艺稳定可行。     

美沙酮维持治疗者社会支持现状及其影响因素调查分析

目的 了解美沙酮维持治疗者的社会支持现状及其影响因素,为建立提高治疗依从性的社会支持系统提供科学依据.方法 采用自拟的一般情况问卷和肖水源的《社会支持评定量表》对246名美沙酮受治者的社会支持现状及其影响因素进行调查,对资料进行描述性分析、t检验和多因素回归分析.结果 受治者的社会支持总分为31.97 ±-8.58,主观支持分为19.38±5.67;不同性别、文化程度、婚姻状况、工作现状受治者的社会支持总分组间比较和主观支持度比较差异均有统计学意义(均有P ＜0.05);多因素Logistic回归分析结果显示,婚姻状况和工作现状为受治者社会支持得分的影响因素(均有P ＜0.05).结论 美沙酮受治者社会支持水平较低,影响美沙酮受治者社会支持得分的主要因素为婚姻状况和工作现状.     

Development and characterization of 20 polymorphic microsatellite loci for the Lhasa schizothoracin Schizothorax waltoni

Schizothorax waltoni is a valued economic fish endemic to Tibet, China. However, there is little information on genetic population structure and genetic diversity for this species. Twenty polymorphic microsatellite loci were isolated and characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet. The number of alleles per locus ranged from 9 to 28 with an average of 18.45. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.716 to 0.942 and from 1.675 to 3.038, respectively. These microsatellite loci are currently being used to evaluate the genetic diversity and genetic population structure to contribute to conservation and management of S. waltoni.     

综合康复护理对老年卧床患者日常生活能力及抑郁症状的影响

目的 探讨综合康复护理对老年卧床患者日常生活活动能力及抑郁症状的应用效果。 方法 选取2018年6－9月在乌鲁木齐市养老福利院居住的128例老年卧床患者,根据卧床不起诊断标准将其分为 A、B、C 三个等级,将不同卧床级别的患者随机分为观察组和对照组。对照组给予常规治疗和护理,观察组在对照组的基础上实施综合康复护理干预,在干预前和干预之后的8周,通过Barthel指数和老年抑郁量表(Geriatric Depression Scale-30,GDS-30)来评估日常生活能力和抑郁症状。 结果 干预前各等级的对照组、观察组患者日常生活能力(activities of daily living,ADL)评分、GDS评分差异无统计学意义(P>0.05)。干预后8周,A、B、C卧床级别的观察组患者Barthel指数评定量表(Barthel Index,BI)ADL评分显著上升[A级:(68.70±5.68)vs.(83.69±8.29), t=11.247, P<0.001;B级:(52.50±5.26)vs.(74.75±9.39) , t=15.112, P<0.001; C级:(31.36±5.81) vs.(34.32±5.19), t=2.891, P=0.009],GDS评分显著下降[A级:(13.39±4.08)vs.(10.04±4.33), t=-3.076, P=0.006;B级:(15.51±4.83)vs.(11.90±4.29) , t=-5.190, P<0.001; C级:(18.23±4.93) vs.(14.50±5.46), t=-3.582, P=0.002],差异均有统计学意义(P<0.05);各级对照组干预前后ADL评分[A级:(68.81±6.51)vs.(70.00±7.07), t=1.588, P=0.135;B级:(53.64±4.92)vs.(55.45±5.96) , t=1.789, P=0.088; C级:(30.75±5.45) vs.(31.25±4.55), t=0.567, P=0.577]、GDS评分[A级:(13.52±4.78)vs.(13.10±4.39) , t=-1.752, P=0.095;B级:(16.36±5.20)vs.(15.14±5.15) , t=-1.618, P=0.121; C级:(19.10±5.95)vs.(19.30±6.03) , t=1.453, P=0.163],差异均无统计学意义(P>0.05)。干预后不同卧床级别观察组患者上述指标均显著优于对照组同期的评估结果,差异有统计学意义(P<0.05)。 结论 综合康复护理能够提高老年卧床患者的日常生活能力,延缓卧床不起的进程,减轻抑郁情绪。     

大黄素配伍中药活性成分体外抗柯萨奇B_3病毒试验

目的:研究大黄素与不同中药活性成分配伍后体外抗柯萨奇病毒B3(CVB3)的作用,并探讨其抗病毒机制。方法:用不同组成、不同浓度的中药材提取液处理CVB3感染的Hep-2细胞,72h后用MTT法测定细胞活性。结果:在Hep-2细胞系中,所有经配伍后的中药组合都能抑制CVB3致细胞病变作用,提高细胞存活率,降低细胞毒性。对病毒直接作用效力最高的药物组合为大黄素:姜黄素:苦参碱=2:1:2;抗病毒合成效力最高的药物组合为大黄素:姜黄素:苦参碱=2:0:1。结论:大黄素与中药活性成分配伍,其细胞毒性降低,抗CVB3效力增强。