Cryoconservation ovarienne : évaluation de deux techniques chirurgicales

ObjectivesTo evaluate various surgical techniques for partial oophorectomy cryopreservation. To evaluate the consequences of prior exposure to cytotoxic therapy on the quality of the ovary removed.Patients and methodsSingle center retrospective observational study over 4 years of women who had ovarian cryopreservation surgery for chemotherapy or radiotherapy which were at high risk of premature ovarian failure. Several techniques have been proposed: partial oophorectomy with clamping of the vascular gonadal pedicle (indirect tissue sample) without clamping (direct tissue sample) and partial oophorectomy with an automatic stapler. Ovarian tissue was immediately prepared for cryopreservation in the operating theatre. The whole sample was divided into small slices. For each ovary, a count of small slices was made. Additionally, one slice was examined to determine the presence of primordial follicles.ResultsOvary was successfully removed and cryopreserved in 13 patients. Two bleeding events occurred with the direct technique, without consequences for patients. The number of fragments obtained between indirect and direct techniques was respectively 19 vs 15, P = 0.18; the number of primordial follicles was 38 vs 36, P = 0.87. The automatic stapler consumed too much ovarian tissue to be interesting. There were fewer fragments, 15 vs 20, P < 0.05 and primordial follicles, 35 vs 40, P = 0.65, after a first cycle of chemotherapy.Discussion and conclusionThe vascular clamping technique is safer but with no difference in the quality of the sample tissue. One cycle of chemotherapy has a pejorative impact on the quality of the sample tissue.     

Characterization and cross-species amplification of 14 polymorphic microsatellite loci in Xenophysogobio boulengeri

The ray-finned fish Xenophysogobio boulengeri is endemic to the upper Yangtze River basin in China. By using the Fast Isolation by Amplified Fragment Length Polymorphism of Sequences Containing repeats protocol, we identified fourteen microsatellite loci in a wild population. The number of alleles per locus ranged from 3 to 15. The observed and expected heterozygosity ranged from 0.125 to 1 and from 0.122 to 0.925, respectively. Cross-amplification was tested in related species X. nudicorpa and Gobiobotia filifer.     

论抗衡新型毒品诱惑的方法

目前,以摇头丸为代表的新型毒品在我国境内已经屡见不鲜,滥用人数也在逐年增加,而且其中还不乏我国社会中的精英群体,“白领”、“金领”、国家公务员等。这些人受过良好的教育,但明知毒品危害却依然吸食,更甚者在生日时借这些新型毒品以飨宾客。现在社会上流传新型毒品成瘾性小,没有什么大不了的,一些年轻人、收入较高的白领就认为摇头丸等新型毒品不是毒,吸点没关系,并无危害,并且得到一定的享受。这种看法增加了我们心理治疗的难度,新型毒品的危害性有别于传统毒品海洛因,他们对于毒品的认知力下降、躯体的戒断反应弱、对心理治疗期望高,精神心理依赖性强,给社会增加了一些不安全因素。武汉小洪山自愿戒毒所针对目前社会上的状况,开设新毒病房,通过实践研制一套心理治疗及心理干预的模式：必须遵循现代的医学模式原则与医学临床紧密结合,帮助患者增强自己的意志活动能力;帮助患者学会控制自己的情绪能力,同时,绝不能忽视完善的支持体系,从身边的小事做起,言行一致去面对现实,改变现实,做一个真正的有感恩心的人。     

微种植钉在安氏Ⅱ类错[牙合]中的临床应用

目的探讨微种植钉在安氏Ⅱ类错[牙合]的临床应用效果。方法研究对象为2010年2月至2012年2月我院接诊的28例安氏Ⅱ类错[牙合]畸形患者,均实施微种植钉治疗,测量并分析正畸相关指标。结果治疗前后的ANB角、Ls-E（上唇突距Sn）、FHtoUI（上前牙和FH平面的夹角）、IMPA（下切牙和下领平面的夹角）等指标的变化可以表明,矫治效果较好,差异均有统计学意义（P〈0．05）。结论微种植钉应用于安氏Ⅱ类错[牙合],可发挥较好的矫治效果,是此类患者首选支抗方案。     

A comparison of potency differences among thyroid peroxidase (TPO) inhibitors to induce developmental toxicity and other thyroid gland-linked toxicities in humans and rats

The potencies of resorcinol, 6-propylthiouracil (PTU) and methimazole (MMI) for inducing developmental toxicity and neurotoxicity were compared in pregnant rats, regarded as valid model for human thyroid toxicity. Profound differences on maternal thyroid hormone levels (THs), maternal toxicity as well as developmental and neurotoxicity sequelae occurred. Resorcinol affected none of those end points. PTU and MMI caused significant effects. Therapy with either PTU or MMI during the first trimester of human pregnancy can cause reductions of maternal THs, accompanied by disruptions of prenatal development. Clinical MMI studies show sporadic evidence of teratogenic effects, with equivocal relation to thyroid peroxidase (TPO) inhibition. In recent decades no MMI associated prenatal toxicity has been reported, an outcome possibly related to carefully managed therapy. Orally administered resorcinol was rapidly absorbed, metabolized and excreted and was undetectable in the thyroid. In contrast, PTU or MMI accumulated. Resorcinol’s potency to inhibit TPO was profoundly lower than that of PTU or MMI. Quantum chemical calculations may explain low resorcinol reactivity with TPO. Thus, distinctions in the target organ and the TPO inhibitory potency between these chemicals are likely contributing to different reductions of maternal THs levels and affecting the potency to cause developmental toxicity and neurotoxicity.     

利培酮口溶膜剂的制备及质量评价

目的：制备利培酮口溶膜剂,并评价其质量。方法：采用流延法制备利培酮口溶膜,以成膜性、外观形状、溶化时限为指标,筛选优化处方。结果：以枸橼酸为稳定剂、羟丙甲基纤维素E15为成膜材料、微晶纤维素和麦芽糊精为填充剂的膜剂可在30 s内溶化,与国外上市产品Risperidon Sandoz^® Solufilm的溶化时限一致,自制样品与国外上市产品在体外4种介质中的溶出行为基本一致,5 min内基本释放完全。结论：利培酮可溶膜处方工艺合理。