PFKFB3在胃癌中的表达及对胃癌细胞生长和凋亡的影响研究

背景与目的:6-磷酸果糖激酶-2同工酶3(6-phosphofructo-2-kinase 3,PFKFB3)与肿瘤的发生、发展密切相关,且对肿瘤细胞的生物学行为具有重要的调节作用。分析PFKFB3在胃癌组织及癌旁组织中的表达,并观察其对胃癌细胞生长和凋亡的作用及机制。方法:采用TCGA数据库分析72例在南昌大学第二附属医院及第三附属医院经外科手术切除的新鲜胃癌标本及其相应的癌旁组织中PFKFB3的表达,并分析PFKFB3的表达与胃癌预后的关系。进一步通过实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)、蛋白质印迹法(Western blot)及免疫组织化学法分析胃癌组织标本及癌旁组织标本中PFKFB3的表达。shNC及shPFKFB3质粒转染至胃癌细胞中,采用RTFQ-PCR和Western blot验证转染效率。分别通过细胞计数试剂盒-8(cell counting kit-8,CCK-8)实验、EdU实验、流式细胞术和Western blot分析PFKFB3下调后对胃癌细胞的增殖能力、细胞周期变化和细胞凋亡的影响。最后采用Western blot分析PFKFB3影响胃癌细胞生长的机制。结果:TCGA数据库分析显示,胃癌组织中PFKFB3的表达明显高于癌旁组织(P<0.05),且PFKFB3高表达与胃癌患者较差的预后密切相关。另外,RTFQ-PCR、Western blot及免疫组织化学检测也同样证实PFKFB3在胃癌组织中高表达(P<0.01),且PFKFB3的表达升高与淋巴结转移及TNM分期密切相关(P<0.05)。沉默胃癌细胞中PFKFB3的表达后,其生长能力明显减弱(P<0.01),胃癌细胞凋亡比例增加(P<0.01),细胞周期G1期阻滞(P<0.01)。PFKFB3通过激活磷酸化磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶B(protein kinase B,AKT)信号通路而调控胃癌细胞的生长。结论:PFKFB3在胃癌组织中高表达,且与患者预后密切相关,沉默PFKFB3的表达后抑制胃癌细胞的生长并促进其凋亡,PFKFB3可能是胃癌靶向治疗的潜在生物标志物。     

The bone marrow-derived mesenchymal stem cells (BMSCs) alleviate diabetic peripheral neuropathy induced by STZ via activating GSK-3β/β-catenin signaling pathway

BackgroundDiabetic peripheral neuropathy, a common complication of diabetic mellitus, has brought a threaten on patients’ health. The bone marrow-derived mesenchymal stem cells (BMSCs) were reported to play an important role in diverse diseases. Nevertheless, the specific function of BMSCs in diabetic peripheral neuropathy remained uncharacterized.MethodsA wide range of experiments including RT-qPCR, western blot, H&E staining, oxidative stress assessment, measurement of thermal sensitivity, ELISA, urine protein and CCK-8 assays were implemented to explore the function and mechanism of BMSCs in vivo and vitro.ResultsThe experimental results displayed that BMSCs improve STZ-induced diabetes symptoms in rats by decreasing blood glucose and urinary protein. Functionally, BMSCs ameliorate oxidative stress, painful diabetic neuropathy, neurotrophic status and angiogenesis in STZ-induced rats. Moreover, BMSCs participate in the regulation of sciatic neuro morphology in diabetic neuropathy rat model. In mechanism, BMSCs alleviate diabetic peripheral neuropathy via activating GSK-3β/β-catenin signaling pathway in rats and improve Schwann's cells viability by activating GSK-3β/β-catenin signaling pathway under high glucose.ConclusionsWe verified that BMSCs alleviate diabetic peripheral neuropathy of rats induced by STZ via activating GSK-3β/β-catenin signaling pathway, which implied a novel biomarker for diabetic peripheral neuropathy treatment.     

头部刮痧辅助治疗外感热病临床效果分析

目的分析探讨头部刮痧辅助治疗外感热病的临床效果。方法以2018年6月-2019年4月收治的126例外感热病患者作为对象进行研究,按照不同治疗方法将患者分为对照组(n=63,给予常规抗病毒、抗炎治疗)与观察组(n=63,在对照组基础上予以头部刮痧辅助治疗)。观察比较2组的治疗效果、退热时间,同时采用疼痛视觉模拟评分法(VAS)评价患者治疗前后头痛程度。结果观察组的退热时间明显短于对照组,治疗总有效率(95.24%)显著高于对照组(82.54%)(P<0.05);2组治疗后30 min、2 h、4 h、6 h的VAS评分均比治疗前降低,但观察组降低程度更优于对照组(P<0.05)。结论基于常规治疗基础上辅以头部刮痧治疗可有效缩短外感热病患者的退热时间,减轻头痛程度,提高患者治疗效果。     

雷帕霉素洗脱支架对急性冠脉综合征患者介入术后C-反应蛋白的影响

目的：比较国产雷帕霉素洗脱支架（Partner^TM）与普通裸支架对急性冠脉综合征患者介入术后C-反应蛋白（CRP）水平的影响。方法；68例急性冠脉综合征患者随机分为两组：A组36例和B组32例。A组置入Partner^TM支架．B组置入普通裸支架。两组分别于术前、术后12、24h取肘静脉血采用胶乳增强免疫比浊法测定C-反应蛋白（CRP）水平。结果：A组术前CRP水平与B组之间差异无统计学意义（P〉0．05）。A组术后12、24hCRP水平与B组比较。差异均有统计学意义（P均〈0．05）。结论；国产雷帕霉素洗脱支架（Partner^TM）对急性冠脉综合征患者介入术后CRP的产生有抑制作用，可能有抑制介入术后的炎症反应。     

腹腔镜胆囊切除术并发症的防治

目的探讨腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)并发症的预防和处理. 方法回顾性总结902例LC并发症种类、处理方法和预防措施. 结果 902例中发生胆总管损伤2例,胆总管结石残留2例,阴囊气肿4例.胆管损伤和胆总管残留结石均经再次手术治愈,阴囊气肿自行吸收.中转开腹手术31例,全组病例均获治愈. 结论腹腔镜胆囊切除术并发症重在预防,适时主动中转开腹是降低LC并发症发生率的关键.     

动态血糖监测下2型糖尿病142例自我管理能力及相关影响因素分析

目的:分析动态血糖监测下2型糖尿病(T2DM)患者自我管理能力及相关影响因素。方法:选取2018年6月至2020年4月中山大学附属南昌医院T2DM患者143例,统计糖尿病自我管理能力量表(SDSCA)评分情况,分析动态血糖监测下T2DM患者自我管理能力现状、影响因素,分析血糖控制情况和SDSCA评分的相关性。结果:本组143例T2DM患者,SDSCA评分为(62.97±4.58)分。不同年龄、病程、月收入、文化程度和是否接受系统化健康教育、有无负性情绪、依从性情况、有无T2DM并发症是动态血糖监测下T2DM患者自我管理能力高低的影响因素(P<0.05);logistic回归分析结果显示,年龄>60岁、病程<2年、月收入<3000元、文化程度初中及以下、负性情绪、T2DM并发症是动态血糖监测下T2DM患者自我管理能力的危险因素,接受系统化健康教育、依从性良好是动态血糖监测下T2DM患者自我管理能力的保护因素(P<0.05);血糖控制良好者SDSCA评分较血糖控制较差者高(P<0.05),血糖控制情况和SDSCA评分呈正相关(r=0.694,P<0.001)。结论:动态血糖监测下T2DM患者自我管理能力整体偏低,影响因素较多,且血糖控制情况和SDSCA评分呈正相关。     

冲击波联合塞来昔布胶囊治疗创伤性踝关节炎30例疗效观察

目的:探究冲击波联合塞来昔布胶囊治疗创伤性踝关节炎的治疗疗效。方法:将2019年1月至2019年12月南昌市第一医院诊治的60例创伤性踝关节炎患者,按随机数字表法分为两组,各30例,对照组给予塞来昔布胶囊治疗,观察组给予冲击波联合塞来昔布胶囊治疗,比较两组患者的临床疗效、踝关节活动范围、骨性关节炎指数评分以及疼痛程度。结果:观察组患者治疗总有效率高于对照组,差异有统计学意义(P<0.05);观察组治疗后踝关节背伸、跖屈、内翻、外翻范围均高于对照组,差异有统计学意义(P<0.05);观察组治疗后骨性关节炎指数(WOMAC)评分低于对照组,差异有统计学意义(P<0.05);观察组治疗1个月、2个月时VAS评分低于对照组,差异有统计学意义(P<0.05)。结论:冲击波联合塞来昔布胶囊治疗创伤性踝关节炎的效果显著,能够增加踝关节的活动范围,提高骨性关节炎指数,缓解疼痛程度,促进患者病情恢复。     

不同煎煮方法对半夏泻心汤中有效成分的影响分析

目的考察去滓再煎法、不去滓煎法、2次煎法3种煎煮方法对半夏泻心汤(BXD)中9种成分黄芩苷、黄芩素、人参皂苷Re、人参皂苷Rb1、甘草酸单铵盐、甘草苷、6-姜辣素、盐酸小檗碱、盐酸巴马汀及总黄酮质量浓度的影响。方法采用HPLC法进行9种指标成分测定,Welch Ultimate XB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,柱温28℃,体积流量0.9 mL/min,检测波长203、252、280、355 nm。采用比色法测定总黄酮。结果检测的9种成分及总黄酮在BXD3种不同的煎煮法中都能测到。去滓再煎法中黄芩苷、黄芩素、人参皂苷Rb1、甘草酸单铵盐、甘草苷比不去滓煎法分别提高了10.01%、12.88%、29.09%、16.75%、15.02%,比2次煎法分别降低了5.54%、4.15%、14.49%、7.85%、9.18%;人参皂苷Re、6-姜辣素、盐酸小檗碱、盐酸巴马汀比不去滓煎法分别提高了37.90%、3.78%、5.33%、5.99%,比2次煎法分别提高了1.07%、11.57%、3.41%、1.93%;总黄酮比不去滓煎法提高了22.61%,比2次煎法提高了6.54%。结论在3种煎法中,去滓再煎的方法明显提高了各成分在水煎液中的含量,从一定程度上提升了BXD的临床功效,为张仲景《伤寒论》中对BXD采用去滓再煎的方法及质量评价提供了实验依据。     

双向Glenn手术的术后护理

报告6例双向Glenn（双向腔静脉肺动脉吻合）手术后的护理经验。6例患者均为复杂先心病，在全麻或全麻体外循环下进行手术，术后严密观察生命体征的变化，包括血流动力学、中心静脉压和血氧饱和度的变化，加强呼吸道管理。全组患者均痊愈出院。术前血氧饱和度平均为76％，术后出院时89％。双向Glenn手术能改善紫绀型先心病患者的缺氧，术后护理是手术成功的关键因素之一。     

Laser fabricated discontinuous anisotropic microconical substrates as a new model scaffold to control the directionality of neuronal network outgrowth

Patterning of neuronal outgrowth in vitro is important in tissue engineering as well as for the development of neuronal interfaces with desirable characteristics. To date, this has been achieved with the aid of micro- and nanofabrication techniques giving rise to various anisotropic topographies, either in the form of continuous or discontinuous structures. In this study we propose a currently unexplored geometry of a 3D culture substrate for neuronal cell growth comprising discontinuous subcellular microstructures with anisotropic geometrical cross-section. Specifically, using laser precision 3D micro/nano fabrication techniques, silicon substrates comprising arrays of parallel oriented elliptical microcones (MCs) were fabricated to investigate whether a discontinuous geometry comprising anisotropic features at the subcellular level could influence the alignment of peripheral nervous system cell populations. It was shown that both Schwann cells and axons of sympathetic neurons were parallel oriented onto the MCs of elliptical shape, while they exhibited a random orientation onto the MCs of arbitrary shape. Notably, this topography-induced guidance effect was also observed in more complex cell culture systems, such as the organotypic culture whole dorsal root ganglia (DRG) explants. Our results suggest that a discontinuous topographical pattern could promote Schwann cell and axonal alignment, provided that it hosts anisotropic geometrical features, even though the sizes of those range at the subcellular lengthscale. The laser-patterned arrays of MCs presented here could potentially be a useful platform for patterning neurons into artificial networks, allowing the study of neuronal cells interactions under 3D ex-vivo conditions.