Redox-sensitive micelles for targeted intracellular delivery and combination chemotherapy of paclitaxel and all-trans-retinoid acid

The application of paclitaxel (PTX) in clinic has been restricted due to its poor solubility. Several traditional nano-medicines have been developed to improve this defect, while they are still lack of tumor targeting ability and rapid drug release. In this work, an amphiphilic polymeric micelle of hyaluronic acid (HA) – all-trans-retinoid acid (ATRA) with a disulfide bond, was developed successfully for the co-delivery of PTX and ATRA. The combination chemotherapy of PTX and ATRA can strengthen the anti-tumor activity. Along with self-assembling to micelles in water, the delivery system displayed satisfying drug loading capacities for both PTX (32.62% ± 1.39%) and ATRA, due to directly using ATRA as the hydrophobic group. Rapid drug release properties of the PTX-loaded redox-sensitive micelles (HA-SS-ATRA) in vitro were confirmed under reducing condition containing GSH. Besides, HA-CD44 mediated endocytosis promoted the uptake of HA-SS-ATRA micelles by B16F10 cells. Due to these properties, cytotoxicity assay verified that PTX-loaded HA-SS-ATRA micelles showed concentration-dependent cytotoxicity and displayed obvious combination therapy of PTX and ATRA. Importantly, HA-SS-ATRA micelles could remarkably prolong plasma circulation time after intravenously administration. Therefore, redox-sensitive HA-SS-ATRA micelles could be utilized and explored as a promising drug delivery system for cancer combination chemotherapy.     

Spontaneous remission of a squamous cell carcinoma of the floor of the mouth

Spontaneous remission is a rare, but well recognized event in oncology. Certain tumours, such as melanomas, hypernephromas and neuroblastomas, are known for showing spontaneous regression. Similarly, spontaneous regression of oral lymphomas, as well as oropharyngeal and recurrent tongue carcinomas, has been reported. Here, we present a novel case of a patient with a primary squamous cell carcinoma on the floor of the mouth whose tumour regressed spontaneously in three months, without any treatment. We also review of the literature on the spontaneous remission of oral cancer and discuss possible mechanisms for this phenomenon.     

非麦角碱类多巴胺受体激动剂缓释制剂的研究进展

帕金森病是常见的神经系统变性疾病,药物治疗仍是目前临床上控制和缓解其症状的主要方法。多巴胺受体激动剂作为治疗帕金森病的新型手段,不仅能在早期改善患者临床症状,而且在晚期显著延缓和减轻运动并发症,因此受到药物研发人员越来越多的关注。持续、稳定的多巴胺受体刺激是控制帕金森病的有效手段,基于此理论,多巴胺受体激动剂缓释制剂发展迅速,主要对其缓释剂型及研究进展进行综述。     

Comparative Botanical and Phytochemical Evaluation of Medicinally Important Stem Bark of Ficus species

ObjectiveTo evaluate the pharmacognostical comparison (Botanical study, physicochemical parameters, HPTLC analysis) in stem barks of four Ficus species (family-Moraceae) viz. F. religiosa L, F. glomerata Roxb, F. retusa auct. & F. carica.MethodsEstimation of Phytochemical markers viz: β-sitosterol and lupeol was quantified by HPTLC method and antioxidant studies by carried by DPPH method.ResultsHPTLC method showed considerable amount of variation with two reference standard viz: β-sitosterol and lupeol content in stem bark of F. religiosa, F. glomerata, F. retusa & F. carica and it were found 0.084, 0.041, 0.059 & 0.131 and 0.020, 0.043, 0.069 & 0.049 respectively. The antioxidant potential of ethanolic extract of stem bark of F. religiosa, F. retusa, F. glomerata & F. carica were found 46.86%, 42.56%, 31.25% & 25.63% at 0.1mg/mL concentration.ConclusionThe present work was taken up with a view to lay down standards which will contribute significantly to quality control of these medicinally useful Ficus species. It also provides suitable criteria to differentiate the stem barks of four Ficus species.     

红景天苷缓释微胶囊的制备及体外释放特性研究

目的:制备红景天苷微胶囊,测定其包封率,并考察其体外释药特性。方法:以生物相容性良好的壳聚糖和海藻酸钠为囊壁材料,红景天苷药物微粒为囊芯,采用静电吸引层层纳米自组装技术(LBL法)制备红景天苷微胶囊。结果:制备的微胶囊包封率较高,达到78.02±0.72%;不同包裹层数的微胶囊在体外释放速度不同。结论:用LBL法制备的红景天苷微胶囊具有较高的包封率和缓释特性,具有一定的应用前景。     

70005/VIP不同天然类敷料在运动性皮肤创伤中应用现状与前景

摘要 目的：评价不同新型天然类敷料的性能特点和在运动性皮肤创伤中应用前景。 方法：以“敷料,天然生物材料,皮肤创伤”为中文关键词;以“surgical dressing, natural biomaterials, leptin and healing of wound”为英文关键词,采用计算机检索1994-01/2010-12相关文章。纳入与有天然累敷料与皮肤创伤相关的文章;共检索到相关文献33余篇。经阅读标题、摘要、全文后,排除内容重复文献筛选后纳入22篇文献进行评价。 结果：传统的敷料虽然有一定的吸湿和保护作用,但愈合过程中易黏连伤口,造成二次损伤,并且止血能力不足,透气性能差等。临床应用或实验的敷料很少由单一原料制备,一般是一种或几种原料共同合成,这样弥补了单一材料的不足并使其各自发挥优势。目前,纳米银敷料、复合材料敷料、细菌合成纤维敷料在动物实验中取得比较好的疗效。但大部分新型敷料还停留在临床试验或试用阶段,各种敷料的原料特点或自身缺陷对其大规模生产和应用还需要进一步研究。 结论：应用于运动性皮肤创伤的敷料的发展将着重在于：一方面要加强止血、止痛、促进创伤快速修复的敷料发展;另一方面敷料能适应各种不同的恶劣环境;同时加强智能敷料的发展。 关键词：敷料;天然生物材料;皮肤创伤;创面愈合;智能敷料 doi:10.3969/j.issn.1673-8225.2011.12.028     

荧光标记多糖的技术及其应用

多糖是生命科学中不可缺少的生物大分子,由于其本身缺少易于检测的发光基团,一直困扰着人们对多糖进一步的研究。荧光物质具有荧光发光基团,通过化学合成的方法将其结合到糖链分子上,从而使多糖带有荧光基团,以便于我们用常用的检测手段进行检测,从而进一步了解多糖发挥的作用,并对其作用机制进行研究。     

不同天然类敷料在运动性皮肤创伤中的应用现状与前景

目的：评价不同新型天然类敷料的性能特点和在运动性皮肤创伤中应用前景。 方法：以“敷料,天然生物材料,皮肤创伤”为中文关键词;以“surgical dressing, natural biomaterials, leptin and healing of wound”为英文关键词,采用计算机检索1994-01/2010-12相关文章。纳入与有天然累敷料与皮肤创伤相关的文章;共检索到相关文献33余篇。经阅读标题、摘要、全文后,排除内容重复文献筛选后纳入22篇文献进行评价。 结果：传统的敷料虽然有一定的吸湿和保护作用,但愈合过程中易黏连伤口,造成二次损伤,并且止血能力不足,透气性能差等。临床应用或实验的敷料很少由单一原料制备,一般是一种或几种原料共同合成,这样弥补了单一材料的不足并使其各自发挥优势。目前,纳米银敷料、复合材料敷料、细菌合成纤维敷料在动物实验中取得比较好的疗效。但大部分新型敷料还停留在临床试验或试用阶段,各种敷料的原料特点或自身缺陷对其大规模生产和应用还需要进一步研究。 结论：应用于运动性皮肤创伤的敷料的发展将着重在于：一方面要加强止血、止痛、促进创伤快速修复的敷料发展;另一方面敷料能适应各种不同的恶劣环境;同时加强智能敷料的发展。