放射性核素介入治疗脑瘤的初步观察

目的　探讨放射性核素3 2 P或198Au介入治疗恶性脑瘤的方法和疗效。方法　介入方法A是术间将放射性核素均匀撒布于残腔壁上 ,B是术中残腔内放置Omaya囊 ,再注入放射性核素 ,3 2 P治疗 7例 ,198Au治疗 1例。结果　症状改善、平稳 ,无严重并发症 ,生存期延长。结论　放射性核素介入治疗脑瘤值得推广应用。     

寡糖对免疫系统作用的研究进展

介绍了寡糖对非特异性免疫系统中巨噬细胞自然杀伤细胞以及补体的激活作用;寡糖对特异性免疫系统中B细胞和T细胞的调节作用;以及寡糖在抗肿瘤、作为人类免疫缺陷病毒(HIV-1)疫苗方面的应用。     

依达拉奉对脑出血大鼠血肿周围脑组织氧化损害保护作用的研究

目的 探讨依达拉奉对脑出血（ICH）后脑组织保护作用。方法 120只SD大鼠随机分为依达拉奉治疗组（依达拉奉治疗组，n=40）、脑出血对照组（ICH对照组，n=40）和假手术组（n=40）。每组分为手术前、术后6h、12h、24h、48h、72h、96h、120h等8个时点，每个时点5只大鼠。采用立体定向自体血注入大鼠尾状核建立ICH模型，观察各组术前及术后各时点血肿周围脑组织丙二醛（MDA）含量及含水量变化。结果 ICH对照组术后各时间点MDA含量显著高于假手术组（P〈0．05），ICH对照组术后各时间点含水量与MDA含量呈明显正相关（r=0．648，P〈0．001）。依达拉奉治疗组术后24-96h各时点MDA含量明显低于ICH对照组（P〈0．001），依达拉奉治疗组术后各时点含水量与MDA含量呈显著相关（r=-0．407，P〈0．05）.结论 脑出血大鼠血肿周围脑组织具有显著的氧化损害，此损害与脑水肿形成有关。依达拉奉使氧化损害明显减轻，依达拉奉对抗脑出血后脑水肿形成可能与清除自由基有关。     

Decorin expression is decreased in first trimester placental tissue from pregnancies with small for gestation age infants at birth

Fetal growth restriction (FGR) is a leading cause of perinatal morbidity and mortality. FGR pregnancies are often associated with histological evidence of placental vascular thrombosis. The proteoglycans are important components and regulators of vascular homeostasis. Previous studies from our laboratory highlighted mRNA and protein expression differences in placental proteoglycan decorin (DCN), within a clinically well-characterised cohort of third-trimester idiopathic FGR compared with gestation-matched uncomplicated control pregnancies. We also showed that decorin contributes to abnormal angiogenesis and increased thrombin generation in vitro. These observations suggest that DCN gene expression may contribute to the etiology of FGR. Small for gestational age (SGA) is frequently used as a proxy for FGR and is defined as a birth weight below the 10th percentile of a birth weight curve. We therefore made use of a unique resource of first trimester tissues obtained via chorionic villus sampling during the first trimester to investigate the temporal relationship between altered DCN expression and any subsequent development of SGA. We hypothesized that placental DCN expression is decreased early in gestation in SGA pregnancies. Surplus chorionic villus specimens from 15 women subsequently diagnosed with FGR and 50 from women with uncomplicated pregnancies were collected. DCN mRNA and DCN protein were determined using real-time PCR and immunoblotting, respectively. Both DCN mRNA and protein were significantly decreased in placentae from first-trimester SGA-pregnancies compared with controls (p < 0.05). This is the first study to report a temporal relationship between altered placental DCN expression and subsequent development of SGA.     

Hepatoprotective effect of mulberry water extracts on ethanol-induced liver injury via anti-inflammation and inhibition of lipogenesis in C57BL/6J mice

Many plant extracts and their bioactive substances are well recognized for their potential to exert as chemoprotective agents against common alcoholic liver injury. In this study, the effects of Mulberry water extracts (MWE) treatment in the prevention of alcohol-induced liver injury were investigated in mice. MWE contain many nutrients and bioactive substances, including fifteen types of polyphenols and anthocyanin compounds. The parameters of histopathology, immunohistochemistry, antioxidant defense and proinflammatory mediator demonstrated the inhibitory effect of MWE on alcohol-induced liver injury. Plasma and hepatic content analysis showed that MWE inhibited the levels of liver injury biomarkers (AST, ALT and ALP), triglyceride (TG) and cholesterol (TC). Furthermore, treatment with MWE lessened the expression of lipid synthesis-related proteins, increased the p-AMPK/AMPK ratio and PPAR-α. Fatty acid oxidation and export via microsomal triglyceride transfer protein (MTP) were both activated as well as carnitine palmitoyltransferase-1 (CPT1). These results suggested that MWE prevents alcohol-induced liver injury through the activation of the AMPK and PPAR-α signal. This may be mediated by multiple pathways, including reduced lipid accumulation and lipid synthesis, increased fatty acid transport and fatty acid oxidation responses, decreased oxidative stress and facilitated anti-inflammation.     

Expression of ferrochelatase has a strong correlation in protoporphyrin IX accumulation with photodynamic detection of bladder cancer

BackgroundThe mechanism underlying the increased levels of protoporphyrin IX in bladder cancer remains unclear. Here, we focus on proteins associated with protoporphyrin IX accumulation in bladder cancer cells and investigate the protein that plays a key role in increased protoporphyrin IX accumulation in bladder cancer cells.MethodsWestern blotting was used to determine the expression of peptide transporter 1, hydroxymethylbilane synthase, ferrochelatase, ATP-binding cassette 2, and heme oxygenase-1 in bladder cancer cell line cells. We evaluated the correlation between the expression of each protein and accumulated protoporphyrin IX in these cells using Pearson's correlation analysis. Immunohistochemistry was used to estimate the expression of the same five proteins in samples from 75 patients who underwent transurethral resection of bladder tumors. The correlation between the expression of each protein in cells from resected bladder specimens and accumulated protoporphyrin IX in bladder cancer cells in voided urine was evaluated using Pearson's correlation analysis.ResultsThe expression of ferrochelatase showed a significant negative correlation with protoporphyrin IX accumulation in vitro (p = 0.04). The expression of peptide transporter 1 (p < 0.01, R = 0.39), heme oxygenase-1 (p < 0.01, R = 0.33), and ferrochelatase (p < 0.01, R = 0.75) in resected bladder specimens by immunohistochemistry was correlated with protoporphyrin IX accumulation in bladder cancer cells in voided urine. On multivariate analysis, the expression of ferrochelatase (p = 0.03) was significant factors to predict positive 5-aminolevulinic acid-induced fluorescent cytology.ConclusionThe expression of ferrochelatase has a strong correlation in protoporphyrin IX accumulation with photodynamic detection of bladder cancer.