Effect of Tiaoxin Recipe on spatial memory and energy metabolism of oxidation injured Alzheimers disease rats

Objective: To observe the effect of Tiaoxin Recipe (TXR) on the spatial memory, brain mitochondrial energy metabolism of oxidation injured Alzheimer’s disease (AD) rats, and to explore the mechanism of TXR in treating AD.Methods: Eighty-eight SD rats were randomly divided into five groups (normal group, operative group, “AD” model group, TXR group and Aricept group). An oxygen free radical generation system (dihydroxy fumaric acid-trichloroferric-adenosine diphosphate, DHF-FeCl₃-ADP) was used to create oxidation injured rat models mimic to AD; spatial learning and memory impairment (Morris water maze method), the activity of Succinate-oxidase, NADH-oxidase, CytC-oxidase (Clark oxygen electrode method) and the expression of cytochrome oxidase (CO) II mRNA (in situ hybridization method) were observed.Results: Compared with the normal group, the spatial memory, activity of CytCoxidase and COII mRNA expression of oxidation injured “AD” rats were obviously decreased; TXR, however, could improve these functions in “AD” rat models obviously.Conclusion: The mechanism of the action of TXR in treating AD was partly related to its effect on anti-oxidation which could improve brain mitochondrial energy metabolism. This program was supported by National Natural Science Foundation of China (No. 39830450)