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排序方式: 共有108条查询结果,搜索用时 18 毫秒
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Zhiqiang Li Tianyong Hou Fei Luo Zhengqi Chang Xuehui Wu Junchao Xing Moyuan Deng Jianzhong Xu 《International orthopaedics》2014,38(11):2391-2398
Purpose
This study focuses on nanoscale self–assembly peptides (SAP) modified demineralized bone matrix (DBM) which provided a more effective osteogenesis and regeneration for critically-sized femur defects in goats using the selective cell retention (SCR) strategy.Methods
RADA16–I peptide was used to modify DBM and formed a composite scaffold (SAP/DBM). The morphological change and dynamic expression of osteogenic genes of mesenchymal stem cells (MSCs) derived from marrow in SAP/DBM was observed. The cells and factors in bone marrow were enriched into SAP/DBM by technology of selective cells retension (SCR). The construct was transplanted into 20-mm femur defects in goats and their osteogenesis was evaluated.Results
The SAP/DBM scaffold formed a three-dimensional interweaving nanofiber in pores of DBM. MSCs exhibited better morphology in SAP/DBM than that in only DBM, and the levels of expression of ALP ,OCN and Runx2 gene in SAP/DBM samples was significantly higher than that of DBM at 14 days in vitro (P?0.05). Compared with marrow-enriched DBM, the volume of newly formed bone from marrow-enriched SAP/DBM is higher in goats (P?0.05).Conclusion
Our study may not only have a significant impact on the construction method of tissue engineering but also provide a viable, simple and effective method for clinical bone construction. 相似文献3.
目的:探讨贝伐单抗辅助培美曲塞联合顺铂化疗治疗非小细胞肺癌的临床应用价值。方法将2007年1月-2008年12月我院收治的240例进展期非小细胞肺癌患者纳入研究,随机分为观察组(给予贝伐单抗+培美曲塞+顺铂化疗)和对照组(给予培美曲塞+顺铂化疗),每组120例,观察和比较两组化疗后的近期疗效、远期疗效以及不良反应的发生情况。结果(1)观察组的客观有效率和疾病控制率均显著高于对照组,差异有统计学意义(P〈0.05);(2)观察组患者的1年、3年、5年生存率均显著高于对照组,无进展生存时间和总生存时间均明显长于对照组,差异有统计学意义(P〈0.05);(3)两组患者不良反应的发生率比较,差异无统计学意义(P〉0.05)。结论贝伐单抗辅助培美曲塞联合顺铂化疗方案有助于促进非小细胞肺癌的缓解,延长患者的生存时间,且不增加不良反应,具有积极的临床应用价值。 相似文献
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Yanjiao Li Hongsuo Liang Dekai Yuan Baoling Liu Ling Liu Yongfa Zhang Kaiyu Hou Yunchao Zhang Bin Chen Jing Ding Yunxia Li Qilin Wang Haiying Wu Hong Shi Min Hu 《Annals of human genetics》2020,84(4):339-344
Osteogenesis imperfecta (OI) is a rare heritable disease with systemic connective tissue disorder. Most of the patients represent autosomal dominant form of OI, and are usually resulting from the mutations in type I collagen genes. However, the gene mutations reported previously only account for ∼70% of the OI cases. Here, in a Chinese OI family, we examined seven patients and nine normal individuals using the whole genome sequencing and molecular genetic analysis. The mutation of rs66612022 (COL1A2:p.Gly328Ser) related to glycine substitution was found in the seven patients. Moreover, we identified a novel missense mutation (HMMR:p.Glu2Gln). Interestingly, the individuals of this family with both the mutations were suffering from OI, while the others carried one or none of them are normal. The mutations of COL1A2 and HMMR and their combined effect on OI would further expand the genetic spectrum of OI. 相似文献
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《Pediatric Dental Journal》2014,24(1):17-21
BackgroundBiochemical markers from saliva and gingival crevicular fluid are of interest in preventing periodontal disease. We investigated the possibility of detecting aspartate aminotransferase (AST) levels in children and teenagers, as well as the usefulness of the addition of measurements of N-bezyoyl-DL-arginine-2-naphthylamide (BANA) activity and AST release during regular oral check-ups.Subjects and methodsFifty-four children (33 males; 8–15 years) from the pediatric dental clinic of Hiroshima University Hospital were enrolled. Periodontal conditions were investigated using scores of the community periodontal index (CPI), BANA and AST. Bacterial species were also identified.ResultsIn 51 eligible subjects, 64.7% were CPI 1 and 35.3% were CPI 2, and these subjects were diagnosed as having gingivitis. A high BANA score was increased in the CPI 2 group (p = 0.022) and the AST score showed a similar trend. All scores of BANA and AST were found in the same CPI. The number of subjects with higher scores of BANA and/or AST increased with increasing age.ConclusionsIt was possible to measure AST levels in children and teenagers. It is suggested that we provide more specific information to children and their guardian using BANA and AST. Measuring biomarkers during regular oral check-ups could lead to an early diagnosis and intervention to avoid the progression of periodontitis during the childhood and teenage years. 相似文献
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目的研究曲马多复合右美托咪定对神经病理性疼痛所致大鼠认知功能障碍和内侧前额叶脑区NR2B表达的影响。方法 50只健康的雄性Wistar大鼠随机分为5组(每组10只),分别为假手术组(SO组),神经病理性疼痛模型组(NP组),模型注射曲马多组(T组),模型注射右美托咪定组(D组),模型注射曲马多+右美托咪定组(T+D组)。神经病理性疼痛模型采用左侧坐骨神经主干慢性压迫法制备。T组、D组和T+D组于术后3天开始分别每天尾静脉注射曲马多注射液(20 mg/kg)、右美托咪定(25μg/kg)、曲马多注射液(5 mg/kg)+右美托咪定(5μg/kg),SO组和NP组以等量生理盐水代替。各组大鼠分别于术后第3天、7天、10天和14天测右侧后足机械缩足阈(MWT)和热缩足潜伏期(TWL)。术后第14天,通过八臂迷宫实验检测大鼠的认知功能变化。八臂迷宫实验完成后立即处死大鼠,通过Western Blot方法检测内侧前额叶脑区NR2B表达水平。结果与SO组比较,NP组的术后痛阈明显降低(P0.05),认知记忆能力明显降低(P0.05),NR2B表达明显升高(P0.05)。与T组和D组比较,T+D组的术后痛阈升高(P0.05),认知记忆能力明显升高(P0.05),NR2B表达明显下降(P0.05)。结论曲马多复合右美托咪定的镇痛效果比单独应用曲马多或右美托咪定效果好,可改善大鼠NP导致的认知功能障碍并使内侧前额叶脑区NR2B表达降低。 相似文献
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目的了解临床护理人员对护理伦理学知识的掌握程度以及应对常见护理伦理问题的现状,为帮助护理人员处理临床护理伦理问题提供依据。方法2011年10~12月,采用自行设计的问卷,对上海市某三级甲等医院110名临床护理人员进行调查。结果护理伦理学知识方面满分11分,总得分为(4.30±1.385);应对护理伦理问题行为方面满分30分,总得分为(26.46±3.233)。结论护理管理者要加强对临床护理人员护理伦理知识的再教育和培训;提高护理人员应对临床护理伦理相关问题的能力。 相似文献
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