Recovery of cryopreserved 96-well plates from −80° C

Summary A simple method is described for the effective recovery of cells cryopreserved in 96-well plates.     

对JAMA“针刺辅助治疗慢性稳定型心绞痛随机临床试验”一文的讨论

成都中医药大学梁繁荣教授团队于2019年7月29日在JAMA内科子刊发表Acupuncture as adjunctive therapy for chronic stable angina:a randomized clinical trial(针刺辅助治疗慢性稳定型心绞痛随机临床试验)的论文,对针灸辅助治疗轻度、中度慢性稳定型心绞痛的安全性、有效性给予肯定。为此,海内外16位专家就该文的研究设计、评估方法、原理机制、临床意义及对未来针灸研究的启示进行了认真的讨论,并对针灸进一步走向世界、获得国际认可提供了一些切实可行的建议。     

Purification of murine IgM monoclonal antibodies by hydroxylapatite chromatography

Summary A simple method is described for the isolation of murine monoclonal immunoglobulin M using hydroxylapatite chromatography.     

A predictive model for the early identification of patients at risk for a prolonged intensive care unit length of stay

Background  

Patients with a prolonged intensive care unit (ICU) length of stay account for a disproportionate amount of resource use. Early identification of patients at risk for a prolonged length of stay can lead to quality enhancements that reduce ICU stay. This study developed and validated a model that identifies patients at risk for a prolonged ICU stay.     

Procedure for intrasplenic immunization of mice

Summary A procedure is described for the direct injection of an immunogen into the spleen to enhance the immune reponse.     

A modified method for glutaraldehyde fixation of cells to a solid phase surface

Summary A method is described for fixing cells to a solid phase surface for use in an antibody detection assay.