用宿主防御指数辅助诊断中医虚证和不同辩证虚证的探讨

以中医学“虚证”及不同辩证虚证为研究对象，以宿主防御指数为研究总指标，开展了营养学和免疫学的多指标观察，并借助于医学统计学的理论和方法，尝试对中医学“虚证”的诊断。结果显示；虚证患者与正常人相比营养条件差，免疫功能氏下，主要表现为细胞免疫功能低下和免疫调节紊乱，补体主要成分降低，将这些评价指标代入计算机进行逐步判断分析，得出可以判别正常人，气阳虚患者，血阴虚患者的方程式，方程式判别能力Ｆ＝３（Ｐ〈     

Inhibitory Effects of the Extracts of Juglans sigillata Green Husks on the Proliferation,Migration and Survival of KYSE150 and EC9706 Human Esophageal Cancer Cell Lines

This study evaluated the antitumor activity of the extracts of green husks of Juglans sigillata Dode on esophageal cancer. KYSE150 EC9706 cells were treated with different concentrations of six components of the extracts of J. sigillata green husks. Cell viability was measured by MTT. Cell migration and cell invasion were measured by wound-healing assays and transwell assays, respectively. Cell apoptosis and cycle were measured by flow cytometry. The expression of cell migration, cell cycle and cell apoptosis regulatory proteins was analyzed by Western blotting. Only the three constituents, including EtOH extractives, EtOAc soluble fraction and gallic acid (GA), exhibited inhibitory effects on the cell viability, migration and invasion by decreasing MMP2 and MMP9 expression (all P?<?0.05). Flow cytometry revealed that these three constituents also induced cell apoptosis by increasing Bax and cleaved caspase-3 but decreasing Bcl-2 in KYSE150 and EC9706 cells. Furthermore, these constituents arrested the cell cycle at G0/G1 by downregulating the expression of Cyclin D1 but upregulating p53 and phospho-p53 expression in KYSE150 cells. In conclusion, the green husks of J. sigillata may act as a potential inhibitor on esophageal cancer growth. GA was the major single active constituent of the extracts.     

LPS－Induced Cellular Immunity ResponseandEffect of Herbal Purgation Method in Intra－Abdominal Infections

ＬＰＳ－ＩｎｄｕｃｅｄＣｅｌｌｕｌａｒＩｍｍｕｎｉｔｙＲｅｓｐｏｎｓｅａｎｄＥｆｆｅｃｔｏｆＨｅｒｂａｌＰｕｒｇａｔｉｏｎＭｅｔｈｏｄｉｎＩｎｔｒａ－ＡｂｄｏｍｉｎａｌＩｎｆｅｃｔｉｏｎｓＬＰＳ－ＩｎｄｕｃｅｄＣｅｌｌｕｌａｒＩｍｍｕｎｉｔｙＲｅｓｐ...     

改良小柴胡汤可能通过转化生长因子-β1/Sma及愤怒相关蛋白信号通路抑制大鼠慢性胰腺炎进展

Objective： To investigate the effect of modified Xiaochaihu Decoction （小柴胡汤, MXD） on transforming growth factor- [3 1/Sma- and Mad-related proteins （TGF- 13 1/Smads） signaling pathway in rats with chronic pancreatitis （CP） induced by dibutyltin dichloride. Methods： Thirty healthy male Wistar rats were randomly divided into the normal control group, CP group and CP＋MXD-treated group. CP was induced by injection of dibutyltin dichloride （DBTC, 7 mg/kg of body weight） into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl-tyrosyl para-aminobenzoic acid （NBT-PABA） test was carried out to evaluate exocrine pancreatic function. Then, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF- β 1, TGF- β 1 type Ⅱ receptor （TGF β R 11 ）, Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. Results： Treatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF- β 1, TGF β R 11 and Smad3 （P〈0.05）. However, MXD had no effect on Smad7 mRNA level. Conclusions： MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-β 1/Smads signaling pathway.     

正交试验优化莪术的乙醇提取工艺研究

目的优化莪术药材中吉马酮和呋喃二烯的乙醇提取工艺。方法采用L9（3^4）正交设计,以吉马酮和呋喃二烯的质量分数为指标,优化莪术的乙醇提取工艺条件。结果最佳的工艺条件为10倍量80％乙醇,提取3次,90min／次。吉马酮和呋喃二烯的质量分数分别达到0．45、0．57mg／g。结论优选的最佳工艺能充分有效地提取莪术中有效成分,具有一定推广价值。     

Modified Xiaochaihu Decoction (加味小柴胡汤) Promotes Collagen Degradation and Inhibits Pancreatic Fibrosis in Chronic Pancreatitis Rats

Objective: To investigate the effect of Modified Xiaochaihu Decoction(MXD, 加味小柴胡汤) on collagen degradation in rats with chronic pancreatitis(CP). Methods: Rats were injected dibutyltin dichloride(DBTC, 7 mg/kg of body weight) into the right caudal vein to induce CP model. Thirty heallhy male Wistar rats were randomly divided into three groups by a random number table: the control, the model and the treatment groups. Rats of treatment group were administered MXD(10 g/kg of body weight) orally once daily starting from the day post-model establishment. Pancreatic tissues were harvested after 28-day feeding and fibrosis was evaluated by picro-sirius red staining. The contents of collagen type Ⅰ and Ⅲ were detected using enzymelinked immunosorbent assay(ELISA), the expression of matrix metalloproteinase 13(MMP13) and tissue inhibitor of metalloproteinase 1(TIMP1) was analyzed by Western blot and real-time polymerase chain reaction(PCR). Results: The fibrosis scoring of pancreatic tissues, the concentrations of collagen type Ⅰ and Ⅲ, the expression levels of MMP13 and TIMP1 proteins and mRNA in the model group were all increased compared with the control group(P0.05). After treatment with MXD, the fibrosis scoring of pancreatic tissues, the concentrations of collagen type Ⅰ and Ⅲ, the expression levels of MMP13 proteins and m RNA in the teatment group were all decreased compared with the model group(P0.05), but there were no significant differences in the expression levels of TIMP1 proteins and m RNA(P0.05). Conclusion: MXD could promote collagen degradation and reverse pancreatic fibrosis in CP rats via a mechanism involve up-regulation of MMP13 expression.     

Prospects for Researchof Acute AbdominalDiseases by Integrated Traditional Chineseand Western Medicine

ＰｒｏｓｐｅｃｔｓｆｏｒＲｅｓｅａｒｃｈｏｆＡｃｕｔｅＡｂｄｏｍｉｎａｌＤｉｓｅａｓｅｓｂｙＩｎｔｅｇｒａｔｅｄＴｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅａｎｄＷｅｓｔｅｒｎＭｅｄｉｃｉｎｅＰｒｏｓｐｅｃｔｓｆｏｒＲｅｓｅａｒｃｈｏｆＡｃｕｔｅＡｂｄｏｍｉ...     

Monocyte subsets study in children with Mycoplasma pneumoniae pneumonia

The aim of this study was to evaluate the changes in the three subsets of monocyte (classical, intermediate, and non-classical) and the expression of human leukocyte antigen-DR (HLA-DR) on monocyte subsets during MP pneumonia in children. Monocyte subsets were analyzed in the peripheral blood of healthy volunteers and MP pneumonia patients at the stages of admission and remission after clinical therapy. They were defined as classical (CD14⁺CD16⁻), intermediate (CD14^brightCD16⁺), and non-classical (CD14^dimCD16⁺) using flow cytometry. Furthermore, three subsets of monocyte were analyzed for the expression of HLA-DR. Patients with MP pneumonia at admission had a higher proportion of intermediate and non-classical monocytes than healthy subjects (all P < 0.05). The proportion of intermediate subset and non-classical subset was lower in MP pneumonia patients at remission than at admission (all P < 0.05). In comparison with the other monocyte subsets, intermediate subset showed a significantly higher percentage of HLA-DR in MP pneumonia patients at admission (P < 0.05). Further analysis revealed that the expression of HLA-DR on intermediate subset was lower in severe patients than in non-severe patients (P < 0.05).Our data has shown for the first time that MP pneumonia is associated with the increased proportion of non-classical and intermediate monocytes, indicating the involvement of monocyte-related mechanisms in the pathogenesis of this disease. Additionally, the decreased expression of HLA-DR on CD14^brightCD16⁺ subset may be a potential indicator of the severity of MP pneumonia.