S100G expression and function in fibroblasts on colitis induction

Supplementation with interleukin (IL)-10, an important anti-inflammatory cytokine, has shown disappointing efficacy for inflammatory bowel diseases (IBD). IL-10 may down-regulate the expression of other anti-inflammatory mediators following colitis induction. We used a colitis model characterized by hapten-protein visualization, which indicates the site of hapten-protein formation after colitis induction for histological and gene expression analyses. Under IL-10 deficiency, following colitis induction inflammatory changes were reduced, and S100G expression was elevated. S100G was expressed in fibroblasts, and S100G expression was down-regulated by IL-10. S100G suppressed the production of monocyte chemotactic protein-1 (MCP-1) through the inhibition of NF-κB activation. Therefore, S100G, also known as Calbindin-D9k, may be an important anti-inflammatory mediator in fibroblasts following colitis induction, and down-regulation of S100G expression might be one reason for the insufficient performance of IL-10 supplementation.     

Sinecatechins Ointment 15% for the Treatment of External Genital Warts

The human papillomavirus (HPV) is one of the most common viral sexually transmitted infections (STIs) in the United States. External genital warts (EGWs) are a commonly seen clinical manifestation of infection with certain strains of the HPV virus, particularly HPV types 6 and 11. Current treatments for the destruction and elimination of EGWs include in -office, provider -delivered therapy and patient -applied medications. Sinecatechins ointment 15% is the newest FDA -approved patient -applied treatment for EGWs. This article reviews the transmission and clinical presentation of EGWs, describes the use of sinecatechins ointment 15% as one patient -applied treatment option and discusses implications for nurses.     

雪峰虫草子座不同生长发育时期的转录组研究

目的：挖掘调控雪峰虫草生长发育的相关通路和基因,揭示其子座生长发育的内在分子机制。方法：采用转录组测序技术（RNA-Seq）对雪峰虫草子座菌丝体（MD1）、子座原基（PD2）和子座（SD3）生长发育3个不同阶段进行测序;数据通过非冗余蛋白（NR）、Swiss-Prot、Pfam、京都基因与基因组百科全书（KEGG）、真核生物相邻类的聚簇（KOG）、基因本体（GO）数据库进行功能注释。利用EdgeR软件进行差异表达基因分析,挖掘调控雪峰虫草子座生长发育的相关通路和基因。结果：测序结果显示,MD1、PD2、SD3分别获得了8.15、7.51、6.87 GB分析数据,拼接分析数据共获得34 218个转录本,平均长度为5502 bp,组装及去冗余后获得10 511条unigenes,平均长度为2470 bp。KOG功能注释和GO富集分析显示,细胞和细胞组分、新陈代谢过程、信号转导及碳代谢是雪峰虫草子座发育过程中的关键基因类别。对子座不同生长发育时期的基因进行差异表达分析发现,PD2和SD3相比差异表达基因最多,其中上调基因1073个,下调基因1036个,MD1和SD3相比差异表达基因最少,上调...     

HPLC法测定阿归养血糖浆中芍药苷含量

目的:建立阿归养血糖浆中芍药苷含量的HPLC测定方法.方法:色谱柱Agilent Tc G18(250mm×4.6mm,5μm);流动相:乙腈-水(14:86);检测波长:230nm.结果:芍药苷的线性范围为0.04856-0.9712 μg(0.9996),平均加样回收率为100.01%,RSD%为0.69%.结论:本方法简便、准确,可用于阿归养血糖浆的质量控制.     

Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling

Repeated observations of enhanced bone growth around various degradable magnesium alloys in vivo raise the question: what is the major mutual origin of this biological stimulus? Several possible origins, e.g. the metal surface properties, electrochemical interactions and biological effects of alloying elements, can be excluded by investigating the sole bone response to the purified major corrosion product of all magnesium alloys, magnesium hydroxide (Mg(OH)₂). Isostatically compressed cylinders of pure Mg(OH)₂ were implanted into rabbit femur condyles for 2–6 weeks. We observed a temporarily increased bone volume (BV/TV) in the vicinity of Mg(OH)₂ at 4 weeks that returned to a level that was equal to the control at 6 weeks. The osteoclast surface (OcS/BS) was significantly reduced during the first four weeks around the Mg(OH)₂ cylinder, while an increase in osteoid surface (OS/BS) was observed at the same time. At 6 weeks, the OcS/BS adjacent to the Mg(OH)₂ cylinder was back within the same range of the control. The mineral apposition rate (MAR) was extensively enhanced until 4 weeks in the Mg(OH)₂ group before matching the control. Thus, the enhanced bone formation and temporarily decreased bone resorption resulted in a higher bone mass around the slowly dissolving Mg(OH)₂ cylinder. These data support the hypothesis that the major corrosion product Mg(OH)₂ from any magnesium alloy is the major origin of the observed enhanced bone growth in vivo. Further studies have to evaluate if the enhanced bone growth is mainly due to the local magnesium ion concentration or the local alkalosis accompanying the Mg(OH)₂ dissolution.     

从骨碎补中制备新北美圣草苷和柚皮苷对照品的研究

目的 建立从骨碎补中制备新北美圣草苷和柚皮苷对照品的方法.方法 结合大孔树脂富集、硅胶柱色谱、中性氧化铝吸附、Sephadex LH-20柱色谱和重结晶方法对骨碎补乙醇提取物进行分离纯化,通过MS、IR、UV、1H-NMR和13C-NMR进行结构鉴定.结果 制备得到的新北美圣草苷和柚皮苷质量分数分别为99.5 %、99.3%.结论 建立的制备方法简单,对照品质量分数高,可作为骨碎补药效物质基础研究和骨碎补药材质量控制的对照品.     

番泻叶不同粉体的化学成分和泻下作用比较

目的研究番泻叶不同粉体的化学成分与泻下作用。方法以燥结缺水便秘小鼠为模型,运用色素流动法,以黑便为指示,通过观测小鼠首次排黑便的时间、稀便点数等指标来比较说明番泻叶不同粉体的泻下作用强弱。利用HPLC法测定番泻叶不同粉体致泻主要活性成分番泻苷A、番泻苷B,比较各粉体中番泻苷A、B的量高低,反映药理活性强弱。结果通过小鼠动物实验观测到,番泻叶超微饮片组首次排黑便的时间明显提前,比饮片组提前了76min,比超微粉组提前了18min;稀便点数增多,比药材饮片组多3.7个,比超微粉组多2.84个。HPLC法测定番泻苷A、B的量显示,超微饮片中番泻苷A和B的质量分数为0.43%,是药材饮片中的1.6倍。结论番泻叶3种粉体的水煎液都能明显增加小鼠腹泻的发生率,尤其以番泻叶超微饮片的通便泻下作用最显著,番泻苷A、B的量最高。     

金银花超微粉体不同极性部位的指纹图谱及其与抑菌活性相关性研究

目的建立金银花超微粉体不同极性部位的指纹图谱,并研究其与抑菌活性的相关性。方法采用系统溶剂提取法得到6个不同极性部位,分别采用HPLC法进行测定,同时选择体外抑菌药理指标进行活性测定;用多元线性回归分析揭示化学信息与抑菌指标的相关关系,寻找与药理指标密切相关的物质群。结果确定以醋酸乙酯提取部位的HPLC指纹图谱来制定金银花的抑菌活性指纹图谱。结论应用该方法建立的金银花粉体的质量标准科学、合理、实用。