脉络膜黑色素瘤脱色素处理后Caspase-3的免疫组化研究

目的 观察凋亡基因Caspase-3在脉络膜黑色素瘤(choroidal melanoma)中的表达及分型与细胞凋亡的关系。方法 收集20例脉络膜黑色素瘤标本，对其进行Caspase-3免疫组织化学染色，观察表达情况及染色强度。结果 Caspase-3在脉络膜黑色素瘤有较好的表达，在梭形细胞型中5例／6例呈阳性表达，在混合型中6例／8例呈阳性表达，在上皮样瘤细胞型中3例／4例呈阳性表达，在坏死型中1例／2例呈阳性表达，且在各型之间没有显著性差异(P＞0．05)。结论 凋亡在脉络膜黑色素瘤中存在；Caspase-3在脉络膜黑色素瘤的发生发展中起重要作用。     

国产达美康治疗Ⅱ型糖尿病的临床观察

本文应用国产达美康对71例NIDDM患者进行了临床观察,同时以优降糖做对照。结果显示:达美康降糖效果与优降糖相当,降血脂方面优于优降糖,并可降低全血粘度,而对照组无此变化,提示达美康在降低血粘度、改善微循环、防治糖尿病性微血管病变上有它独特的作用。结果表明达美康是治疗NIDDM安全而有效的口服降糖药。     

171例高血压患者血脂,脂蛋白及载脂蛋白水平的分析

本文就171例HBP患者血脂、脂蛋白及载脂蛋白水平与155例正常人进行比较分析发现:HBP患者WI、TG及apoB较对照组显著升高,TC无明显差别,HDL-C、HDL_2-C及apoAI显著降低、正常男性TC、TG和apoB随年龄增长呈升高趋势,女性TC、TG和LDL-C则在50岁以后开始升高。而HBP患者除男性apoB随年龄增加而升高外,其余各项,不分男女,均无随年龄增减而变化的趋势。     

苦参碱对人HT1080细胞系增殖抑制的体外研究

目的：研究苦参碱（Matrine)对人成纤维肉瘤（HT1080）细胞系生长的抑制效应，并探讨其作用机制。方法：用不同浓度的Mat加入体外培养HT1080细胞中，观察加药后细胞生长数量及其形态的变化；用MTT法检测Mat的细胞毒作用。结果：Mat明显抑制HT1080细胞生长；IC50值为350μg/ml；其抑制率与药物浓度呈剂量依赖关系，结论：Mat能有效抑制HT1080细胞的增殖。     

A study on standard 12-lead ECG QRS scoring system for estimating myocardial infarct size]

Using the standard 12-lead electrocardiographic QRS scoring system modified by Nancy (1985), 99 ECGs of 52 patients with acute myocardial infarction were used for estimating myocardial infarct size (MIS). One point was designed to represent 3% of the left ventricle. Each patient had the first score of ECG at the day ranged from 7 to 12 and the second 25-32 days post acute chest pain except 5 cases died before the second scoring. The results showed that the averaged score was 6.7 +/- 3.1 points accumulated from all 99 ECGs and 11.0 points from 5 cases died. There was no statistic difference between the points value of the first scoring 6.4 +/- 2.9 vs second 6.5 +/- 2.7 points, nor between the points received from Q waves 4.0 +/- 1.5 vs 3.9 +/- 1.4 points in 47 patients. There was nevertheless strong correlation both between the total points of the first and second scoring, r = 0.85 (P less than 0.0005), and those between the points related to Q wave, r = 0.81 (P less than 0.005). Furthermore, early serial serum CPK changes were determined for evaluating MIS in 25 patients, the results were compared to the first and second scores respectively, the correlations were significant, r = 0.58, P less than 0.0025 and r = 0.47, P less than 0.01.(ABSTRACT TRUNCATED AT 250 WORDS)     

Transtemporal supralabyrinthine approach]

Transtemporal supralabyrinthine approach is a modified middle cranial fossa approach. It offers all the advantages of a middle cranial fossa procedure and avoids its disadvantages, mainly the extensive temporal lobe retraction and frightening complications. The principle of the approach is to gain sufficient access toward the internal auditory canal by removing bone from the roof of the petrous pyramid rather than by elevating the middle fossa dura away from it. Fifteen patients underwent this approach for decompression of paralysed facial nerve resulted from temporal bone fracture, Bell's palsy and herpes zoster oticus; for removal of facial neuromas and primary cholesteatomas in temporal bone and for sectioning of great superficial petrous nerve. Preliminary study showed good results.     

miR-139-3p在低氧诱导凋亡的心肌细胞中的表达及其作用研究

目的:探讨微小RNA-139-3p(miR-139-3p)在低氧诱导的原代心肌细胞凋亡模型中的表达及其意义。方法:在正常和低氧条件下,采用RT-qPCR检测乳大鼠原代心肌细胞miR-139-3p的表达水平;进一步将miR-139-3p抑制剂和miR-139-3p抑制剂阴性对照转染到心肌细胞后,将细胞置于37℃密闭的缺氧盒中(95%N_2和5%CO_2)培养12 h,采用流式细胞术和Western blot法检测细胞凋亡情况。结果:低氧培养12 h后,与正常培养组(n=3)比较,低氧组(n=3)心肌细胞中的miR-139-3p相对表达量显著上调(P0.05)。低氧组心肌细胞凋亡率也显著升高(P0.05)。与miR-139-3p抑制剂阴性对照组(n=3)比较,miR-139-3p抑制剂组(n=3)的心肌细胞凋亡率显著降低(P0.05)。结论:miR-139-3p在低氧诱导的原代心肌细胞凋亡模型中表达上调。抑制miR-139-3p的表达能降低低氧诱导的心肌细胞凋亡。     

大鼠肝细胞泌乳素受体的研究

本文以~(125)I-oPRL为放射催标记配体,利用受体放射分析法,对哺乳期大鼠肝细胞泌乳素受体进行了研究。结果表明。大鼠肝细胞泌乳素受体与泌乳素的结合具有高度特异性和可饱和性,而且受反应时间和pH值大小的影响;这种结合并非简单的双分子反应,大鼠肝细胞泌乳素受体很可能存在两种亲和力高低不同的结合位点(Kd_1=3.20×10~(10)M,Kd_2=1.26×10~(-8)M)或负协同调节。本研究为将来深入开展泌乳素对肝脏机能调节机制的探讨打下了基础。