人和兔雪旺氏细胞的体外培养及形态学观察和生物特性研究

雪旺氏细胞(Schwann cell,SC)在神经再生过程中起着重要作用。我们取人胚及兔之坐骨神经,采用“植块多次移出法”培养出纯净度达99％的人和兔的SC。对培养的SC进行了冻存和复苏处理,复苏后的细胞可保持原有的生长特性。     

高温抑制舌癌细胞侵袭及转移机理的实验研究

目的探讨高温治癌与基质金属蛋白酶系统之间的关系,以期阐明高温抑制肿瘤细胞侵袭及转移的机理.方法对舌癌细胞Tca-8113进行体外43℃加温,通过免疫组化、流式细胞术及明胶酶谱活性分析等方法进行分析,以研究加温后该舌癌细胞MMP-9、MMP-2表达量及其活性的改变.结果加温与未加温细胞MMP-9、MMP-2均有表达,但加温后的细胞MMP-9和MMP-2蛋白表达量减少,并且加温后的细胞MMP-9和MMP-2的活性较未加温细胞明显下降.结论高温能通过调节基质金属蛋白酶(MMP-9、MMP-2)的表达量及其活性来抑制肿瘤细胞的侵袭、转移能力.     

肺癌患者纤支镜取材测定c-erbB-2蛋白的临床意义

目的 探讨肺癌患者经纤支镜取材，检测c—erbB—2蛋白的临床意义。方法 对肺部有占位性病变的患者行纤支镜取材，再用免疫组化S—P法检测c—erbB—2蛋白的表达情况，并比较肺癌组、肺良性病变组c—erbB—2蛋白的表达情况及其与临床病理因素的关系。结果 肺癌组、肺良性病变组c—erbB—2蛋白的阳性表达率分别为35．0％、10．0％，有显著性差异；c—erbB—2蛋白与肺癌组织学类型、分化程度、淋巴结及远处转移有关。结论 术前纤支镜取材测定c—erbB—2蛋白的表达情况有助于肺癌的临床诊断、分期及预后的判定。     

小狼毒的二萜化学成分研究(Ⅰ)

从大戟科小狼毒Euphorbiaprolifera根的乙醇提取物中分得2个二萜化合物，通过光谱分析鉴定为3，5，8-三乙酰基-5α-苯甲酰基-14-丙酰基曼西醇类二萜（3，5，8-triacetyl-5α-benzoyl-14-propanoylmyrsinoltypediterpenewithC9～C10cyclizedtoformanadditionallactonering，Ⅰ），3-乙酰基-5β，8α-二苯甲酰基-14-丙酰基曼西醇类二萜（3-acetyl-5β，8α-dibenzoyl-14-propanoylmyrsinoltypediterpenewithC9～C10cyclizedtoformanadditionallactonering，Ⅱ）。其中Ⅱ为新化合物。     

Endocrine Dysfunction Following Stroke

Endocrine dysfunction is known to occur after traumatic brain injury. The purpose of this study was to examine the incidence of various endocrine dysfunctions after a stroke. The Taiwan National Health Insurance Research Database (NHIRD) was searched from 2001 to 2011 for patients with a diagnosis of stroke. Stroke patients were matched by diagnosis date, age, and sex to patients without a stroke. Cox proportional hazards regression analyses were performed to compare the incidence of goiter, acquired hypothyroidism, thyroiditis, pituitary dysfunction, and disorders of the adrenal glands between stroke and non-stroke patients. There were 131,951 patients in the stroke group, and 131,951 in the matched non- stroke group (mean age 66.1 ± 14.9 years). Stroke patients had significantly higher risk of acquired hypothyroidism (crude hazard ratio [cHR] = 1.65, 95% confidence interval [CI]: 1.44, 1.90; adjusted hazard ratio [aHR] = 1.65, 95% CI: 1.42, 1.91), pituitary dysfunction (cHR = 2.32, 95% CI: 1.79, 2.99; aHR = 1.92, 95% CI: 1.46, 2.52), and disorders of the adrenal glands (cHR = 1.79, 95% CI: 1.52, 2.12; aHR =1.62, 95% CI: 1.36, 1.92) than non-stroke patients. Pituitary dysfunction and disorders of the adrenal glands were found in both hemorrhagic stroke and ischemic stroke patients, while hypothyroidism was seen in ischemic stroke patients only. No significant association was found for goiter and thyroiditis. In conclusions, stroke survivors have an approximately 2-fold increased risk of developing acquired hypothyroidism, pituitary dysfunction, or disorders of the adrenal glands. These risks should be taken into account in the management of patients who have ischemic or hemorrhagic strokes.

Graphical Abstract

     

Relationships between electrochemical skin conductance and kidney disease in Type 2 diabetes

BackgroundSUDOSCAN® non-invasively measures peripheral small fiber and autonomic nerve activity using electrochemical skin conductance. Since neuropathy and nephropathy are microvascular Type 2 diabetes (T2D) complications, relationships between skin conductance, estimated glomerular filtration rate (eGFR), and urine albumin:creatinine ratio (UACR) were assessed.MethodsTwo hundred five African Americans (AA) with T2D, 93 AA non-diabetic controls, 185 European Americans (EA) with T2D, and 73 EA non-diabetic controls were evaluated. Linear models were fitted stratified by population ancestry and T2D, adjusted for covariates.ResultsRelative to EA, AA had lower skin conductance (T2D cases p < 0.0001; controls p < 0.0001). Skin conductance was also lower in T2D cases vs. controls in each population (p < 0.0001, AA and EA). Global skin conductance was significantly associated with eGFR in AA and EA with T2D; adjusting for age, gender, BMI, and HbA1c, positive association was detected between skin conductance and eGFR in AA T2D cases (parameter estimate 3.38, standard error 1.2; p = 5.2E^− 3), without association in EA T2D cases (p = 0.22).ConclusionsNoninvasive measurement of skin conductance strongly associated with eGFR in AA with T2D, replicating results in Hong Kong Chinese. SUDOSCAN® may prove useful as a low cost, non-invasive screening tool to detect undiagnosed diabetic kidney disease in populations of African ancestry.     

心缩间期与心功能指标的相关关系及其随龄变化

本文主要研究左室心缩间期与心功能指标的相关关系及其随龄变化。以多普勒超声心动图检测了１２６例健康成人左室射血前期、左室射血时间及左室加速时间等心缩间期指标，并与同时测算的心脏收缩与舒张功能指标、年龄、身高、体重、血压水平、心腔内径、室壁厚度等可能的影响因素进行直线相关和多元逐步回归分析。结果显示心缩间期指标与心脏收缩功能指标间存在显著性相关关系，与舒张功能指标间的相关关系也有统计学显著性；年龄增长是左室射血前期及左室加速时间的独立影响因素。结论:心缩间期可作为评估心功能简便易行的无创性指标。其随年龄增长的变化在评估心功能时应予考虑。心缩间期与心脏舒张功能间的内在联系有待深入研究