Lipopolysaccharide-binding protein as a biomarker in oral and maxillofacial tumors

Oral and maxillofacial tumors (OMTs), such as oral squamous cell carcinoma (SCC), pleomorphic adenoma, and ameloblastoma, are common head and neck tumors. Lipopolysaccharide-binding protein (LBP) is a type I acute reactive protein, which participates in body inflammatory response modulation through lipopolysaccharide (LPS)-induced signaling pathway by targeting macrophages (expressing cluster of differentiation 204 [CD204]). Although it is well established that LBP is associated with the development of multiple types of cancer, little is known about the role of LBP in OMTs. This study aims to explore the expression of LBP in OMTs. Here, immunohistochemical (IHC) double staining of LBP and CD204 and enzyme-linked immunosorbent assay (ELISA) were conducted to explore the LBP expression in OMTs. The findings demonstrated that the LBP expression in OMTs was significantly elevated (p < 0.001). In addition, the LBP expression was associated with the clinical stage (p < 0.001), T classification (p < 0.001), and lymph node metastasis (p < 0.001, except ELISA) but independent of histological grade of SCC, gender, and age in patients with SCC. The optional cutoff of the LBP serum level is 0.721 μg/ml. To conclude, LBP contributes to the development of OMTs and could be a biomarker in the screening and predicting metastasis in patients with OMTs.     

Clinical features of Danon disease and insights gained from LAMP-2 deficiency models

Danon disease (DD) is an X-linked multisystem disorder with clinical features characterized by the triad of hypertrophic cardiomyopathy, skeletal muscle weakness, and mental retardation. Cardiac involvement can be fatal in the absence of an effective treatment option such as heart transplantation. Molecular studies have proved that LAMP-2 protein deficiency, mainly LAMP-2B isoform, resulting from LAMP2 gene mutation, is the culprit for DD. Autophagy impairment due to LAMP-2 deficiency mediated the accumulation of abnormal autophagic vacuoles in cells. While it is not ideal for mimicking DD phenotypes in humans, the emergence of LAMP-2-deficient animal models and induced pluripotent stem cells from DD patients provided powerful tools for exploring DD mechanism. In both in vitro and in vivo studies, much evidence has demonstrated that mitochondria dysfunction and fragmentation can result in DD pathology. Fundamental research contributes to the therapeutic transformation. By targeting the molecular core, several potential therapies have demonstrated promising results in partial phenotypes improvement. Among them, gene therapies anticipate inaugurate a class of symptom control and prevention drugs as their in vivo effects are promising, and one clinical trial is currently underway.     

中青年甲状腺癌术后患者自我管理积极度及影响因素分析

目的 了解中青年甲状腺癌术后患者自我管理积极度状况并分析影响因素，为制定针对性护理干预措施提供参考。 方法 采用便利抽样法抽取就诊的中青年甲状腺癌术后患者268例，使用一般情况调查表、患者积极度量表、社会影响量表、疾病接受度量表进行调查。 结果 中青年甲状腺癌术后患者积极度得分为（57.48±16.57）分。患者积极度总分与病耻感总分呈负相关，与疾病接受度总分呈正相关（均P＜0.05）。多重线性回归分析显示，文化程度、131 I 治疗、病耻感、疾病接受度是中青年甲状腺癌术后患者积极度的影响因素（均P＜0.05）。 结论 中青年甲状腺癌术后患者积极度处于中等水平。医护人员应根据中青年甲状腺癌术后患者自我管理积极度的影响因素制定系统、有效的护理干预策略，以提高其自我管理积极度水平。     

Original antigenic sin: not so sinful after all

‘Original antigenic sin’ predicts that antibody responses to secondary infections with escape mutants are dominated by specificities to the original pathogen. Using transgenic mice in which antibodies are tagged based on cellular origins and kinetics, Schiepers et al. support this prediction, and reveal accumulation of cross-reactive specificities chiefly among long-lived responses.     

Temporary Vascular Occlusion with Retrievable Microvascular Plugs to Protect Normal Liver during Yttrium-90 Radioembolization

This case series describes a technique to protect nondiseased liver parenchyma during transarterial radioembolization (TARE) using microvascular plugs to occlude nontarget vessels temporarily and protect normal liver. This technique, defined as temporary vascular occlusion, was performed in 6 patients, with complete vessel occlusion obtained in 5 of the 6 patients and partial occlusion with flow reduction in 1 of the 6 patients. A statistically significant (P = .001) dose decrease of 5.7 ± 3.1 times was measured using postadministration yttrium-90 positron emission tomography/computed tomography in the protected zone compared with that in the treated zone.     

3D打印技术在骨肉瘤围手术期治疗中的应用进展

骨肉瘤发病部位涉及复杂的解剖结构，精准切除肿瘤并保留周围重要神经和血管，以及骨肿瘤切除后骨缺损的修复重建等方面均具有挑战性。3D打印技术可“量体裁衣”般地适配不规则骨肿瘤切除后的“骨缺损”，达到良好肢体重建的治疗效果。本文对近年来3D打印技术在骨肉瘤术前、术中、术后重建、骨组织工程支架中的应用进展进行评述，并根据3D打印的特征、优点及目前存在的问题，对未来骨肉瘤患者精准化和个体化治疗提出了参考意见。     

miR-132-3p promotes heat stimulation-induced esophageal squamous cell carcinoma tumorigenesis by targeting KCNK2

Epidemiological evidence supports that consumption of high-temperature food and beverages is an important risk factor for esophageal squamous cell carcinoma (ESCC); however, the underlying mechanism still remains unclear. Here, we established a series of animal models and found that drinking 65°C water can promote esophageal tumor progression from preneoplastic lesions to ESCC. RNA sequencing data showed that miR-132-3p was highly expressed in the heat stimulation group compared with controls. Further study verified that miR-132-3p were upregulated in human premalignant lesion tissues of the esophagus, ESCC tissues, and cells. Overexpression of miR-132-3p could promote ESCC cell proliferation and colony formation, whereas knockdown of miR-132-3p could inhibit ESCC progression in vitro and in vivo. Importantly, dual-luciferase reporter assays showed that miR-132-3p could bind with the 3′-untranslated region of KCNK2 and inhibit KCNK2 gene expression. Knockdown or overexpression of KCNK2 could promote or suppress ESCC progression in vitro. These data suggest that heat stimulation can promote ESCC progression and miR-132-3p mediated this process by directly targeting KCNK2.