过表达RECK基因对成釉细胞瘤hTERT＋_AM永生化细胞株MMPs表达及侵袭能力的影响

目的：观察转染RECK基因对人成釉细胞瘤（ameloblastoma,AM）细胞株hTERT-AM的MMPs表达及细胞侵袭力的影响。方法：利用RECK基因慢病毒载体Lenti—RECK—eGFP／puro转染hTERTAM细胞株．Puro筛选和镜下挑单克隆纯化细胞。CCK8检测细胞活性,Transwell实验检测细胞迁移、侵袭能力,qRT—PCR检测细胞中RECK的mRNA的表达情况。Western蛋白印迹检测细胞中RECK、MMP2、MMP9的表达情况。采用SPSS13．0软件包对数据进行统计学分析。结果：转染RECK基因后,hTERT~AM细胞中RECK的mRNA和蛋白的表达均显著增高（P均〈0．01）,细胞活性无显著变化（P均〉0．05）,细胞迁移、侵袭能力均显著下降（P均〈0．01）,MMP2、MMP9蛋白表达均显著下降（P均〈0．05）。结论：RECK基因参与人成釉细胞瘤局部侵袭的调节,过表达RECK基因后,MMP2、MMP9下调,抑制AM细胞迁移和侵袭。RECK有望成为人成釉细胞瘤侵袭防治的新靶点。     

弥散张量成像显示亚急性期脑梗死神经网络损伤及各运动参数分析

背景：脑卒中后神经网络与运动功能的相关性尚未明确。 目的：应用磁共振弥散张量成像研究亚急性期脑梗死后神经网络受损情况,并分析其与神经功能缺陷及运动功能障碍的相关性。 方法：将19例亚急性期脑梗死患者和20名正常成人分别进行弥散张量成像检查,分析比较以下参数：各向异性分数、表观扩散系数和各向异性分数指数、表观扩散系数指数。同时对患者进行神经功能缺损和运动功能的各项量表评估,检测10 m步行速度。将脑梗死患者弥散张量成像的各项参数与各项量表及10 m步行速度进行相关性分析。 结果与结论：脑梗死患者各向异性分数指数和双侧内囊后肢的各向异性分数值均小于正常对照,且患侧内囊后肢的各向异性分数值小于健侧内囊后肢的各向异性分数值（P〈0.05）。患侧内囊后肢的表观扩散系数值、表观扩散系数指数大于正常对照内囊后肢的表观扩散系数值和表观扩散系数指数（P〈0.05）。患侧内囊后肢的表观扩散系数值、表观扩散系数指数与下肢Fugl-Meyer评分呈负相关（P 〈0.05）。提示弥散张量成像参数与下肢运动功能障碍密切相关。脑卒中后的局灶性病变造成神经网络缺损,是下肢运动功能障碍的主要原因。     

性传播感染导致的直肠炎、直肠结肠炎和肠炎的诊治要点——2021年美国CDC《性传播感染诊疗指南》分析

性传播感染可导致有症状的直肠炎、直肠结肠炎和肠炎,且其管理中存在较多问题,2021-07-23美国CDC发布了最新版《性传播感染诊疗指南》,本文结合最新文献以及临床经验,重点解读该指南较上一版指南的不同之处,并将该指南中性传播感染导致的直肠炎、直肠结肠炎和肠炎治疗指南部分与《2021版性传播病原体引起的直肠炎、直肠结肠炎和肠炎的欧洲管理指南》部分内容进行对比,旨在为临床医生管理性传播感染所致的直肠炎、直肠结肠炎和肠炎提供帮助。     

De-escalating Locoregional Therapy for Axillary Micrometastases in Breast Cancer: How Much is Too Much?

BackgroundThe applicability of modern prospective data on adjuvant radiotherapy (RT) fields in patients with micrometastases is limited because many trials occurred prior to routine measurement of nodal metastasis size and modern sentinel lymph node evaluation techniques. We aimed to determine prognostic factors for patients with micrometastases and evaluate the impact of adjuvant RT on disease outcomes.Patients and MethodsPatients diagnosed with pathologic T1-T3 N1mi breast cancers between 2004-2015 were identified. Cox proportional hazards methods were used to determine characteristics predictive of locoregional recurrence (LRR). Tumor and treatment-specific factors were further evaluated using log-rank statistics to compare rates of LRR-free survival.ResultsThis analysis included 156 patients. On multivariable analysis, grade 3 histology (HR 10.84, 95% CI 2.72-43.21) and adjuvant RT (HR 0.22, 95% CI 0.06-0.81) were independent predictors of LRR. Among patients with grade 1-2 histology, 5-year LRR-free survival was 98.8% in patients who received adjuvant RT versus 100% in patients who did not receive adjuvant RT (P = .82). Among patients with grade 3 histology, 5-year LRR-free survival was 90.1% in patients who received adjuvant RT versus 53.0% in patients who did not receive adjuvant RT (P = .025), and 100% in patients receiving comprehensive nodal irradiation versus 76.7% in patients receiving whole breast irradiation or no RT (P = .045).ConclusionPatients with grade 3 micrometastases are at substantial risk for LRR. Adjuvant RT, including comprehensive nodal irradiation, should be strongly considered in these women.     

Sketch guided and progressive growing GAN for realistic and editable ultrasound image synthesis

Ultrasound (US) imaging is widely used for anatomical structure inspection in clinical diagnosis. The training of new sonographers and deep learning based algorithms for US image analysis usually requires a large amount of data. However, obtaining and labeling large-scale US imaging data are not easy tasks, especially for diseases with low incidence. Realistic US image synthesis can alleviate this problem to a great extent. In this paper, we propose a generative adversarial network (GAN) based image synthesis framework. Our main contributions include: (1) we present the first work that can synthesize realistic B-mode US images with high-resolution and customized texture editing features; (2) to enhance structural details of generated images, we propose to introduce auxiliary sketch guidance into a conditional GAN. We superpose the edge sketch onto the object mask and use the composite mask as the network input; (3) to generate high-resolution US images, we adopt a progressive training strategy to gradually generate high-resolution images from low-resolution images. In addition, a feature loss is proposed to minimize the difference of high-level features between the generated and real images, which further improves the quality of generated images; (4) the proposed US image synthesis method is quite universal and can also be generalized to the US images of other anatomical structures besides the three ones tested in our study (lung, hip joint, and ovary); (5) extensive experiments on three large US image datasets are conducted to validate our method. Ablation studies, customized texture editing, user studies, and segmentation tests demonstrate promising results of our method in synthesizing realistic US images.     

Elevated activity in the dorsal dentate gyrus reduces expression of fear memory after fear extinction training

Background:Effectively reducing the expression of certain aversive memories (fear or trauma memories) with extinction training is generally viewed to be therapeutically important. A deeper understanding of the biological basis for a more effective extinction process is also of high scientific importance.Methods:Our study involved intraventricular injection or local injection into the dorsal dentate gyrus of anti-neuregulin 1 antibodies (anti-NRG1) before fear extinction training, followed by testing the expression of fear memory 24 hours afterward or 9 days later. We used local injection of chemogenetic or optogenetic viruses into the dorsal dentate gyrus to manipulate the activity of the dorsal dentate gyrus and test the expression of fear memory. We also examined the effect of deep brain stimulation in the dorsal dentate gyrus on the expression of fear memory.Results:Mice that received intraventricular injection with anti-NRG1 antibodies exhibited lower expression of fear memory and increased density of activated excitatory neurons in the dorsal dentate gyrus. Injection of anti-NRG1 antibodies directly into the dorsal dentate gyrus also led to lower expression of fear memory and more activated neurons in the dorsal dentate gyrus. Inhibiting the activity of dorsal dentate gyrus excitatory neurons using an inhibitory designer receptor exclusively activated by designer drugs (DREADD) eliminated the effects of the anti-NRG1 antibodies. Enhancing the activity of the dorsal dentate gyrus with an excitatory DREADD or optogenetic stimulation resulted in lower expression of fear memory in mice that did not receive infusion of anti-NRG1 antibodies. Deep brain stimulation in the dorsal dentate gyrus effectively suppressed expression of fear memory, both during and after fear extinction training.Limitations:The mechanism for the contribution of the dorsal dentate gyrus to the expression of fear memory needs further exploration.Conclusion:Activation of the dorsal dentate gyrus may play an important role in modulating the expression of fear memory; its potential use in fear memory extinction is worthy of further exploration.     

单核细胞/高密度脂蛋白胆固醇比值对下肢动脉狭窄的诊断价值

目的 探讨外周血单核细胞/高密度脂蛋白胆固醇比值(MHR)对下肢动脉狭窄的诊断价值.方法 选取2018年8月-2020年8月在中山大学附属第八医院就诊的连续性病例311例,根据下肢动脉狭窄程度不同将患者分为4组:(1)对照组(n=65):下肢动脉正常;(2)轻度病变组(n=77):30％≤下肢动脉狭窄＜50％;(3)中...     

Y染色体短串联重复序列基因座荧光复合扩增体系的法医学应用

目的探讨Y染色体DYS456、DYS464、DYS527、DYS531、DYS709、DYS448和DYS522共7个短串联重复序列(STR)基因座(相当于11个位点)荧光复合扩增体系在法医学中的应用价值。方法提取法医学应用中生物检材的基因组DNA,复合扩增7个Y-STR基因座,然后采用毛细管电泳和聚丙烯酰胺凝胶电泳法检测扩增产物。结果盲测试验结果正确;可作为"人"与"非人"的种属特异性检测工具;用同一个体不同组织所获得的分型结果一致;调查同一父系家庭的男性成员样品,未观察到变异;对强奸案混合斑的分析,可直接检出7个Y-STR基因型,协助确定嫌疑人。结论荧光复合扩增Y染色体7个STR基因座方法可靠,对混合斑和微量检材中男性DNA的分析具有较高的应用价值。     

LCoR、NRIP1蛋白在宫颈癌组织中的表达及与临床病理、预后的关系分析

目的 探讨配体依赖辅助抑制因子(LCoR)、核受体相互作用蛋白1(NRIP1)在宫颈癌组织中的表达及与患者临床病理、预后的关系.方法 选取2015年1月至2016年6月于江门市新会区妇幼保健院接受手术治疗的124例宫颈癌患者作为研究对象.术中取癌组织及癌旁组织(距肿瘤组织边缘≥5cm),采用免疫组化法检测组织中LCoR...     

HBV X蛋白对阿霉素诱导的肝癌细胞凋亡的影响

目的：探讨乙型肝炎病毒X蛋白（hepatitis BvirusX protein,HBVX）对阿霉素诱导的肝癌细胞凋亡及p53、PTEN表达的影响。方法：用阿霉素（2.5g/mL）分别处理HepG及稳定表达GFP、GFP-HBVX融合蛋白的细胞系HepG/GFP、22HepG/GFP-HBVX,处理后不同时间在显微镜下观察细胞形态变化,流式细胞术检测细胞凋亡。结果：流式细胞术检测显2示阿霉素处理后36h,HepG/GFP-HBVX细胞凋亡率为3.94%,明显低于HepG（59.03%）、HepG/GFP细胞（61.38%）222（P〈0.05）,而与未处理对照组细胞（2.12%、2.78%、2.55%）无明显差别（P〉0.05）。结论：HBVX蛋白能够抑制阿霉素诱导的细胞凋亡。