HPLC-ELSD法测定三维肌醇片中肌醇的含量

目的：建立简便准确的测定三维肌醇片中肌醇含量的方法。方法：采用HPLC-ELSD方法进行测定,色谱柱为Ultimate^TM XB-NH2（4．6mm×250mm,5μm）,流动相为乙腈．水（70：30）,流速0．8mL·min^-1,漂移管温度100℃。雾化气流速度2．5L·min^-1。结果：肌醇在0．1016～1．016mg·mL^-1范围内线性关系良好,r=0．9997,平均回收率102．32％,RSD=1．41％,样品中其他成分不干扰测定。结论：本方法简便可靠,可用于复方制剂中肌醇含量的质量控制。     

对1例乳腺癌患者的药学监护

1例47a女性患者,因右乳癌术后3年,发现全身多发骨转移3月余入院。入院后,给予多西他赛联合顺铂方案化疗,常规水化和利尿止吐,化疗期间加用伊班膦酸钠抗骨转移治疗。临床药师把化疗过程中可能出现的不良反应的预防与处理及药物选择作为药学监护点,建议医生常规使用泮托拉唑保护胃黏膜,避免奥美拉唑对多西他赛代谢的影响。建议选用肾毒性较小的伊班膦酸钠,以减轻患者肾损害,患者最终顺利完成本周期治疗。     

肺癌细胞致敏树突状细胞疫苗的体内外抗肿瘤作用

目的:研究以NCI-H460肺癌细胞致敏的树突状细胞(Dendritic cells,DCs)疫苗的抗肿瘤作用。方法:将IL-18基因转染的NCI-H460细胞及未转染NCI-H460细胞与DC融合作为转染融合DC组及融合DC组疫苗,以NCI-H460细胞RNA冲击的DC为冲击DC组疫苗,以未冲击的DC为DC组疫苗。以MTT法检测4组DC疫苗刺激T细胞增殖的作用,用ELISA法测定DC疫苗上清液中IL-12的含量,用LDH法测定DC疫苗对NCI-H460细胞的杀伤作用。裸鼠皮下接种NCI-H460细胞及DC疫苗,观察成瘤时间及裸鼠存活情况;荷瘤裸鼠瘤内注射DC疫苗,比较肿瘤体积。结果:4组DC疫苗均能刺激T细胞增殖,刺激作用强度为转染融合DC组>融合DC组>冲击DC组>DC组;4组均能分泌IL-12,转染融合DC组IL-12的分泌量高于融合DC组,冲击DC组高于DC组;转染融合、融合、冲击DC和DC组疫苗对NCI-H460细胞的杀伤率分别为79.73%、50.68%、35.81%及4.05%。转染融合组裸鼠移植瘤成瘤时间[(12.82±2.85)d]长于冲击DC组[(8.52±1.97)d](P<0.05)和DC组[(8.33±1.63)d](P<0.01);移植瘤内注射疫苗后的肿瘤体积比较,转染融合组<融合组<冲击DC组相似文献   

In vivo real-time visualization of mesenchymal stem cells tropism for cutaneous regeneration using NIR-II fluorescence imaging

Mesenchymal stem cells (MSCs) have shown great potential for cutaneous wound regeneration in clinical practice. However, the in vivo homing behavior of intravenously transplanted MSCs to the wounds is still poorly understood. In this work, fluorescence imaging with Ag₂S quantum dots (QDs) in the second near-infrared (NIR-II) window was performed to visualize the dynamic homing behavior of transplanted human mesenchymal stem cells (hMSCs) to a cutaneous wound in mice. Benefiting from the desirable spatial and temporal resolution of Ag₂S QDs-based NIR-II imaging, for the first time, the migration of hMSCs to the wound was dynamically visualized in vivo. By transplanting a blank collagen scaffold in the wound to help the healing, it was found that hMSCs were slowly recruited at the wound after intravenous injection and were predominantly accumulated around the edge of wound. This resulted in poor healing effects in terms of slow wound closure and thin thickness of the regenerated skin. In contrast, for the wound treated by the collagen scaffold loaded with stromal cell derived factor-1α (SDF-1α), more hMSCs were recruited at the wound within a much shorter time and were homogenously distributed across the whole wound area, which enhances the re-epithelialization, the neovascularization, and accelerates the wound healing.     

Albuminuric diabetic kidney disease predicts foot ulcers in type 2 diabetes

Diabetic Foot Ulcers (DFU) are feared among individuals with diabetic kidney disease (DKD), but it is unclear whether they are more frequent, especially in normoalbuminuric DKD.Five hundred and twenty patients admitted in our diabetology ward from 2007 to 2017 were followed up during 54 ± 26 months. New DFUs were registered, and their relationship with the initial renal status was analyzed by LogRank and multivariate Cox regression analysis.The 520 subjects were mainly men (57.9 %), 62 ± 9 years old, with a duration of diabetes of 14 ± 10 years, HbA1c: 8.7 ± 1.8 % (72 ± 19 mmol/mol), and complications: 33.7 % macroangiopathies, 22.1 % previous foot ulcers, 44.8 % DKD, 26.9 % retinopathies. Fifty-seven new DFU occurred, mainly in subjects with DKD. DKD was related to later DFU (HR: 1.79; 95%CI: 1.05–3.07), this relationship stayed significant adjusted for age, gender, and a history of previous DFU (HR: 3.61; 95%CI: 2.11–6.18), and further adjusted for the duration of diabetes, HbA1c, BMI, arterial hypertension, and dyslipidemia. Among the 233 subjects with DKD, 129 (55.3 %) had an isolated AER > 30 mg/24H, 41 (17.6 %) had an isolated eGFR<60 mL/min/1.73 m², and 63 (27.0 %) cumulated both abnormalities. By Cox regression analysis adjusted for age and gender, albuminuric DKDs were related to later DFU: with eGFR≥60: HR: 1.91; 95%CI: 1.02–3.59, with eGFR<60: HR: 2.53; 95%CI: 1.25–5.10, whereas normoalbuminuric DKD was not: HR: 1.04; 95%CI: 0.35–3.07, despite similar rates of neuropathies, peripheral arterial diseases, and retinopathies.In people with type 2 diabetes, albuminuric DKD was associated with two to three folds increased risk of DFUs, whereas normoalbuminuric DKD was not.     

YTHDF2 inhibition potentiates radiotherapy antitumor efficacy

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