T‐helper cell‐mediated factors in drug‐induced liver injury

Drug‐induced liver injury (DILI) leads to a large burden on the healthcare system due to its potential morbidity and mortality. The key for predicting and preventing DILI is to understand the underlying mechanisms. Hepatic inflammation is one of the most common features of DILI. The inflammation can be attributed to the innate immune response. The adaptive immune system is also affected by the innate immune response resulting in liver damage. T‐helper cells are important regulators of acquired immunity. T‐helper cell‐mediated immune responses play pivotal roles in the pathogenesis of a variety of liver disorders. This review summarizes recent advances in the T‐helper cell‐mediated factors in DILI and potential mechanisms, which may lead to a better understanding of DILI. Copyright © 2015 John Wiley & Sons, Ltd.     

HPLC指纹图谱和UPLC-Q-TOF-MS方法对芪冬颐心口服液中成分研究

目的 建立芪冬颐心口服液的HPLC指纹图谱,结合UPLC-Q-TOF-MS方法对其成分进行归属,为芪冬颐心口服液质量控制提供理论依据。方法 建立10批次芪冬颐心口服液指纹图谱,采用“中药指纹图谱相似度评价系统（A版）”进行相似度评价,方法学考察精密度、重复性、稳定性,同时应用UPLC-Q-TOF-MS方法对其成分进行鉴别和归属。结果 10批次芪冬颐心口服液指纹图谱中有7个共有峰,指纹图谱相似度大于0.99。同时应用UPLC-Q-TOF-MS方法,正离子质谱数据鉴定出25个成分,负离子质谱数据鉴定出17个成分,正、负离子质谱数据共同鉴定出9个成分,并对每个共有峰进行单味药材归属。结论 HPLC指纹图谱结合UPLC-Q-TOF-MS方法准确可靠,重复性和稳定性较好,可为芪冬颐心口服液的质量控制提供参考。