辅助生殖技术助孕患者伴侣亲密关系与产前抑郁的相关性研究

目的 调查辅助生殖技术助孕患者伴侣亲密关系与产前抑郁现状,探讨伴侣亲密关系对辅助生殖技术助孕患者产期抑郁的影响,为改善助孕患者产前心理状况和健康干预提供依据。 方法 于2017年1月-10月,采用便利抽样法研究,选择杭州市5所三级医院行辅助生殖技术助孕患者共426人为研究对象,应用婚姻调适测定量表调查助孕患者伴侣亲密关系,应用爱丁堡产后抑郁量表调查患者的产前抑郁现状;用SPSS 18.0统计学软件进行数据处理;采用相关性分析分析辅助生殖技术助孕患者伴侣亲密关系与产前抑郁的相关性。 结果 426例助孕患者亲密关系得分为(104.43±15.85)分,亲密关系良好者229例(53.76%);爱丁堡产后抑郁量表得分为(8.52±4.18)分,抑郁发生率为24.41%;Pearson相关性分析结果显示,辅助生殖技术助孕患者伴侣亲密关系与产前抑郁呈负相关(r=-0.395,P=0.008),差异具有统计学意义。 结论 辅助生殖技术助孕患者伴侣亲密关系与产前抑郁呈负相关,即患者伴侣亲密关系水平越高,其产前抑郁水平越低,社会、医院、家庭可以从伴侣亲密关系着手进行干预,以降低辅助生殖技术助孕患者产前抑郁水平。      

单亲二体及其在癌症中的作用研究进展

单亲二体（UPD）是指个体的同源染色体或其上的一部分均来自于双亲中一方的一种染色体缺陷,能够造成基因印记障碍或基因纯合突变,从而导致各类疾病,包括癌症。UPD的形成机制多样,主要由细胞分裂时染色体未正确分离所致。UPD不改变基因拷贝数,导致传统的细胞遗传学检测手段难以对其进行有效鉴定。近年来,随着单核苷酸多态性阵列等技术的出现,越来越多的UPD相关病例被检出,并发现其以非随机的形式出现在不同类型的癌症中,在癌症的发生、发展以及转移等过程中发挥重要作用。本文从UPD的形成机制、检测方法、影响区域、涉及基因、临床预后价值等方面,综述UPD在癌症中的作用研究进展,并讨论其发展趋势。     

Quantitative determination of anti-tumor agent bis(4-fluorobenzyl)trisulfide, fluorapacin and its pharmaceutical preparation by high-performance liquid chromatography

A simple isocratic and stability-indicating HPLC method was developed and validated for the quantitative determination of anti-tumor agent fluorapacin and its pharmaceutical preparation. A Spherisorb ODS II C(18) (250 mm x 4.6 mm, 5 microm) column was eluted with a mobile phase consisting of acetonitrile/water (85:15, v/v). The analyses were performed at 40+/-1 degrees C with a flow rate of 1.0 mL/min and UV detection at 218 nm. The calibration curve was linear over a concentration range of 160-240 microg/mL with the correlation coefficient of 0.9997. The LOD and LOQ were determined to be 1.4 and 7.0 ng/mL, respectively. Average recoveries were 98.27% and 100.40% for fluorapacin API and its drug product with corresponding relative standard deviations (R.S.D.) of 0.41% and 0.30%, respectively. Good repeatability (precision and intermediate precision), accuracy and tolerability were obtained with R.S.D. of <1.0%. This specific and reliable method has been successfully applied for quality control of fluorapacin API and drug product.     

Effect of autogenic training on quality of life and symptoms in people living with HIV: A mixed method randomized controlled trial

ObjectiveQuality of life of people living with HIV is strongly affected by sleep problems, fatigue, pain, anxiety, and depression symptoms. This study set out to evaluate the effects of autogenic training (AT) on quality of life and symptoms within this population.MethodsA mixed method randomized controlled trial was conducted. Participants were randomly assigned to either the AT group (n = 32) or the control group (CG) (n = 31). Quality of life and symptoms were measured in both groups three times: prior to intervention (T0), immediately after intervention (T3), and three months after intervention (T6). Fourteen individual interviews were conducted.ResultsResults show a significant improvement in social and mental dimensions of quality of life for the two groups at T6. They also show a significant improvement in sleep for AT participants at T3. Qualitative results are consistent with quantitative ones.ConclusionAT seems to improve sleep quality and could improve some dimensions of quality of life and other symptoms among people living with HIV. Further studies are needed to confirm these results.Trial registration numberNCT01901016.     

High-performance liquid chromatographic mapping of the oligosaccharides released from the humanised immunoglobulin,CAMPATH ™1H

A sensitive and reproducible method for the routine mapping of oligosaccharides in a humanised immunoglobulin (IgG) is described.The method involves the enzymic release of intact glycans using the endoglycosidase glycopeptidase-F, and subsequent derivatisation with 1-phenyl-3-methyl-5-pyrazolone to facilitate analysis by high-performance liquid chromatography (HPLC). The heterogeneous oligosaccharide chains are separated by a phosphate buffer-acetonitrile gradient reversed-phase HPLC method and monitored by ultraviolet detection at 245 nm, allowing the detection of picomole amounts. A number of standard oligosaccharides are similarly derivatised to enable classification of the types of structures present from a comparison of retention times.     

关于医院无线网络建设和验收的探讨

在随着近年来数字化医院概念的提出和建设,无线局域网的部署在医院信息化进程中提上了日程。无线局域网作为移动医疗信息系统的网络平台,先进性和实用性极其重要。在如今无线局域网验收和测试缺乏标准的现状下,通过对无线局域网几种架构的技术分析,结合考虑医院可能的应用场景,提出部署设计建议和相关验收测试方法。