Cryoablation for liver metastasis from solid pseudopapillary tumor of the pancreas: A case report

BACKGROUND Solid pseudopapillary tumor(SPT) of the pancreas is a rare pancreatic tumor and 10% to 15% of cases are associated with metastasis. Cryoablation is a new method that can induce tumor necrosis, and treatment of tumors by cryoablation can cause anti-tumor immune responses.CASE SUMMARY A 16-year-old woman with SPT of the pancreas developed liver metastases 5.3 years after complete resection of the primary pancreatic tumor. She was admitted with chief complaints of abdominal pain in the upper abdomen and a weight loss of approximately 5 kg over 4 mo. Carbohydrate antigen(CA) 125,carcinoembryonic antigen, and CA 199 were normal. An abdominal computed tomography scan found multiple nodules in the right lobe of the liver that measured approximately 13.5 cm × 10.8 cm × 21.4 cm. Immunohistochemical staining results showed that CD10 and CD56 were positive, and the patient was diagnosed with SPT of the pancreas with liver metastasis. The patient underwent percutaneous cryoablation and interventional embolization. During the 5-year follow-up, the patient remained disease-free after cryoablation, with relatively normal immune function.CONCLUSION Herein, we for the first time report the treatment of liver metastasis from SPT of the pancreas using cryoablation plus interventional embolization, which could be a promising alternative therapy for pancreatic SPT liver metastasis.     

多重定量荧光PCR在21-三体及性染色体多倍体畸变诊断中的应用

目的探讨多重定量荧光PCR(QF-PCR)技术在染色体非整倍体畸变诊断中的应用价值。方法抽取脐血样本16份,羊水样本73份,21、X、Y染色体数目异常患者及其父母外周静脉血71份,针对21号染色体和X、Y染色体上7个基因位点21S1435、D21S11、D21S1411、AMXY、DXS981、DXS6809和X22应用QF-PCR方法进行多重扩增,毛细管电泳法检测并分析结果。所有样本同时进行染色体核型分析。结果染色体核型分析中有129例为正常核型46,XX(XY);26例21-三体(其中1例为易位型,余为标准型);1例45,XO/46,XX;2例47,XXX;1例47,XXY;1例45,XX,der(13,21)。QF-PCR结果中,确诊1例47,XXY,26例21-三体征中23例确诊,2例47,XXX被提示可能存在性染色体数目异常,其余为阴性结果。结论多重定量荧光技术可用于染色体非整倍体畸变的快速诊断。     

Association of serotonin transporter gene polymorphisms and major depressive disorder in Chinese Han population

BACKGROUND： Serotonin transporter （5-HTT） polymorphisms comprise 5-HTT gene-linked polymorphism region （5-HTTLPR） and variable number of tandem repeats （VNTR）. Studies have revealed an association between 5-HTT polymorphism and major depressive disorder, which suggests that the ＂S＂ allele of 5-HTTLPR and Stin2.9 of 5-HTTVNTR are associated with major depressive disorder. However, there are a number of studies that do not support the 5-HTT polymorphism effect in major depressive disorder. OBJECTIVE： To study the relationship between 5-HTT gene polymorphism and major depressive disorder in Chinese Han population. DESIGN, TIME AND SETTING： Case-controlled study of 5-HTT gene polymorphism. The experiment was performed at the Central Laboratory, Third Affiliated Hospital of Sun Yat-sen University, China from March 2005 to January 2006. PARTICIPANTS： A total of 99 depressive patients of Chinese Han nationality were recruited for this study. All patients met DSM-IV diagnostic criteria for major depressive disorder and had a total score of Hamilton Depression Scale （24 items） ≥21 points. In addition, 101 healthy subjects, matched for age and gender, served as the control group. METHODS： Venous blood was collected from all subjects. 5-HTT genotypes and alleles were determined by polymerase chain reaction. Consistent with the Hardy-Weinberg equilibrium, the association between 5-HTT gene polymorphism and major depressive disorder were analyzed by Chi-square test. MAIN OUTCOME MEASURES： 5-HTTLPR and 5-HTTVNTR genotypes and allele frequencies were measured. RESULTS： No significant differences in 5-HTTLPR genotypes and allele frequencies were determined between patients and controls （P 〉 0.05）. However, significant differences in 5-HTTVNTR genotypes and allele frequencies were detected （P 〈 0.01 ）. The Stin2.10 allele and 10/10 genotype associated with major depressive disorder （OR = 2.61,7.7, P 〈 0.05; analysis of dose-response relationships Х^2 = 12.35, P 〈 0.01）. CONCLUSION： Results from the present study revealed no association between 5-HTTLPR and major depressive disorder. However, a significant association between 5-HTTVNTR and major depressive disorder existed in a population of Chinese Han. The presence of Stin2.10 and 10/10 genotypes increased the risk for major depressive disorder in a dose-dependent manner.     

江西汉族女性BRCA2基因单核苷酸多态性与散发性乳腺癌易感相关性研究

目的:探讨BRCA2基因单核苷酸多态性(SNP)与江西汉族女性散发性乳腺癌易感性的关系。方法:利用SequenomMassArrayiPLEX GOLD系统对江西南昌大学第一附属医院散发性乳腺癌患者152例和健康对照组165名的BRCA2基因编码区3个单核苷酸多态性位点(rs766173,rs144848,rs1801426)进行基因型检测,非条件Logistic回归分析。结果:rs766173位点基因型和等位基因型频率分布差异均有统计学意义(χ2=4.602,P=0.032;χ2=4.097,P=0.043)。相对TT基因型,TG杂合型与TG+GG型均能显著性增加乳腺癌发生的危险性,OR值(95%CI)分别为1.971(1.060~3.666)和1.934(1.052~3.555),P分别为0.032和0.034。相对等位基因T,等位基因G为易感等位基因,OR值为1.792(95%CI=1.012~3.172,P=0.045)。另外两个位点rs144848和rs1801426多态性分布频率在乳腺癌组和对照组之间差异无统计学意义。结论:rs766173位点基因多态性与江西地区汉族女性散发性乳腺癌易感性相关,另外两个多态性位点rs144848和rs1801426与江西地区女性散发性乳腺癌易感性无关。     

广陈皮的超临界流体萃取和水蒸气蒸馏挥发油的比较分析

目的:比较两种不同提取方法所得的广陈皮挥发油的挥发性化学成分。方法:分别采用超临界CO2流体萃取(SFE)和水蒸气蒸馏(SD)的方法提取广陈皮的挥发油。运用气相色谱-质谱联用法分离和分析两挥发油的挥发性化学成分,主要成分棕榈酸和D-柠檬烯采用标准品的峰面积增大法进一步确认。结果:从广陈皮SFE和SD挥发油中共鉴定出22个成分,占色谱总流出峰面积的97%以上,两者的提取率分别为5.05%和0.46%。SFE挥发油的主要成分为挥发性较弱、相对分子量较大的棕榈酸,SD挥发油的主要成分为挥发性较强、相对分子量较小的D-柠檬烯。此外,SFE和SD挥发油中均有含量不低的2-甲胺基-苯甲酸甲酯。结论:采用两种方法提取广陈皮所得挥发油的挥发性成分不尽相同,且SFE法的提取率高于SD法;两种方法均能将广陈皮的特征性成分2-甲胺基-苯甲酸甲酯提取出来。     

反相高效液相色谱法同时测定冬虫夏草中腺苷、虫草素、肌苷含量方法的研究

目的建立同时测定冬虫夏草中腺苷、虫草素、肌苷的HPLC方法。方法采用RP-HPLC法,色谱柱：Agilent C18柱（250 nm×4.6 mm,5μm）,甲醇与水梯度洗脱,柱温：30℃,流速：1.0 mL.min-1,检测波长：260 nm。结果腺苷进样量在0.200 8~1.004μg与峰面积呈良好线性关系,回归方程为Y=2 876X＋0.458 8,r=0.998 7,平均加样回收率为100.5%,RSD为2.33%;虫草素进样量在0.202 2~1.011μg与峰面积呈良好线性关系,回归方程为Y=2 018X＋8.726,r=0.999 2,平均加样回收率为99.3%,RSD为2.03%;肌苷进样量在0.201 4~1.007μg与峰面积呈良好线性关系,回归方程为Y=5 845X-5.685,r=0.999 4,平均加样回收率为100.2%,RSD为1.44%。结论本方法简便、准确,重复性好,可用于同时检测冬虫夏草中的腺苷、虫草素及肌苷的含量测定。     

健康教育在未婚女大学生行无痛人工流产术中的应用

目的探讨对行无痛人工流产术的未婚女大学生实施健康教育的效果。方法对行无痛人工流产术的1015例未婚女大学生实施健康教育,观察实施后效果。结果 1015例患者术程顺利,均表示健康教育能减轻其负性情绪和心理负担;术后将更好保护自己,将主要精力用于完成学业中。结论对实施无痛人工流产的未婚女大学生进行健康教育具有重要的意义,急需学校、社区、媒体等多个层面共同参与。     

经脐单孔与常规腹腔镜阑尾切除手术的比较研究

目的:探讨单孔腹腔镜阑尾切除手术的可行性。方法:对2011年1月—2011年12月收治的122例急性阑尾炎病例,按照随机原则分单孔腹腔镜组(61例)和常规腹腔镜组(61例),对两组的手术时间、术中出血量、肠道功能恢复时间、住院时间、并发症、中转率进行比较。结果:两组在术中出血量、术后肠道功能恢复时间、住院时间及术后并发症的比较,差异均无统计学意义(P>0.05),单孔腹腔镜组因粘连(2例)和腹膜后阑尾(1例)中转常规腹腔镜手术;坏疽性(穿孔性)阑尾炎的手术时间,单孔腹腔镜组明显长于常规腹腔镜组(P=0.004);单纯性阑尾炎和化脓性阑尾炎两组的手术时间无统计学差异(P>0.05)。结论:选择性对急性阑尾炎行单孔腹腔镜手术治疗是安全可行的;与常规腹腔镜比较,单孔腹腔镜治疗单纯性阑尾炎和化脓性阑尾炎同样具有恢复快、创伤小的优点,且比常规腹腔镜有更好的维护形体的效果。     

Maitland手法结合牵引推拿TDP治疗神经根型颈椎病的临床研究

目的观察Maitland手法结合牵引、推拿、特定电磁波辐射仪(TDP)治疗神经根型颈椎病(cervical spondylotic radicu-lopathy,CSR)的临床疗效和安全性。方法采用随机对照临床试验的方法,将146例CSR患者分为治疗组、对照A组和对照B组进行3个疗程(30 d)的治疗观察。治疗组采用Maitland手法、牵引、推拿、TDP疗法治疗,对照A组采用推拿、牵引、TDP疗法治疗,对照B组采用牵引、TDP疗法治疗。比较3组临床疗效及疼痛评分、颈椎功能评分改善情况。结果治疗结束时,治疗组、对照A组和对照B组的总有效率分别为97.9%、85.7%和65.3%;1个月后随访,3组的总有效率分别为93.7%,77.6%和57.1%;治疗组的总有效率优于对照A组和对照B组(均P0.01)。治疗组疼痛评分差值和颈椎评分差值分别为4.5±1.5和10.4±3.5,对照A组的分别为3.2±1.9和7.6±2.9,对照B组的分别为2.4±1.5和5.2±2.9,治疗组的疼痛评分差值和颈椎评分差值优于对照A组和对照B组(均P0.01)。结论 Maitland手法结合牵引、推拿和TDP治疗CSR具有一定临床时效性、科学性,值得推广。     

多重实时荧光定量PCR检测急性肝炎患者HBV和HCV方法的建立与意义分析

目的建立一种新的可以快速筛查急性肝炎患者HBV和HCV感染的多重实时荧光定量PCR方法。方法收集596例急性肝炎患者血清或血浆样本,应用自主构建的多重实时荧光定量PCR方法同时检测HBV和HCV,同时应用HBV和HCV单管检测试剂进行检测分析。结果新建立的多重实时荧光定量PCR体系,可以有效地检测HBV和HCV的阳性质控品及阳性样本。多重实时荧光定量PCR体系特异性较好,检测HBV和HCV时,两者之间无交叉反应,且以多种血浆病毒的核酸样品为模板进行检测时结果全为阴性;多重实时荧光定量PCR体系检测HBV、HCV的灵敏度分别为20 IU/ml、50 IU/ml;多重实时荧光定量PCR体系精密度好,检测HBV、HCV的CV值分别为0.11%、0.096%。在检测临床样本时,多重复合体系检测血清或血浆中HBV和HCV的检出率与单独检测HBV和HCV具有很好的一致性,且HBV和HCV的一致率分别达到97.7%(k=0.952)、98.8%(k=0.976);同时有6例样本出现HBV和HCV共感染。结论新型多重实时荧光定量PCR方法对快速检测及筛查HBV和HCV较快捷,而且特异性好、灵敏度高、精密度好。因此多重实时荧光定量PCR方法对2种肝炎病毒的检测具有重要意义。