氯胺酮对离体大鼠心室肌细胞瞬时外向钾电流的影响

目的研究氯胺酮对大鼠心室肌细胞瞬时外向钾电流(Ito)的影响。方法酶解法分离大鼠心室肌细胞,采用全细胞膜片钳技术记录Ito,观察50μmol/L氯胺酮对Ito电流-电压曲线以及不同浓度氯胺酮对Ito的影响,并研究氯胺酮对Ito通道动力学的影响。结果钳制电压-40mV,刺激电压 70mV条件下,临床相关浓度的氯胺酮50μmol/L使Ito的电流峰值降低23·4%(P<0·01),冲洗后,Ito能够完全恢复。5、10、50、100、500、1000、5000μmol/L的氯胺酮抑制Ito呈浓度依赖性,电流抑制率分别为(13·8±9·7)%、(17·5±6·7)%、(23·4±8·8)%、(31·5±6·7)%、(63·3±5·5)%、(79·7±2·7)%、(88·9±4·4)%,其半数有效浓度(IC50)为299μmol/L。100μmol/L的氯胺酮对激活曲线没有影响;使Ito的失活曲线明显右移,半数失活电压(V1/2)在给药前后数值分别为(-28·27±0·20)mV和(-25·34±0·27)mV(P<0·01),斜率因子(k)值分别为(3·23±0·46)mV和(3·40±0·55)mV(P>0·05)。结论氯胺酮可明显阻滞大鼠心室肌的Ito,这是氯胺酮延长大鼠心室肌动作电位的机理之一,同时氯胺酮使Ito的失活曲线右移。     

Diterpenoids from the Roots of Agastache rugosa

从土藿香根中得到两个二萜化合物，藿香酚（２）及异藿香酚（３）。经光谱解析（ＩＲ，ＵＶ，ＨＲＭＳ及ＮＭＲ谱），确定它们的结构分别为１１，１４ｄｉｈｙｄｒｏｘｙ１２ｍｅｔｈｏｘｙ１９（４→３）ａｂｅｏａｂｉｅｔａ４，（１８），８，１１，１３ｔｅｔｒａｅｎ７ｏｎｅ（２）和１１，１４ｄｉｈｙｄｒｏｘｙ１２ｍｅｔｈｏｘｙ１９（４→３）ａｂｅｏａｂｉｅｔａ３，８，１１，１３ｔｅｔｒａｅｎ７ｏｎｅ（３）藿香酚（２）为一新的化合物，而异藿香酚（３）系首次从天然界分离得到，二者为一对异构体。     

外阴汗腺癌七例临床分析

目的：总结外阴汗腺癌的临床特点及治疗方法。方法：对７例外阴汗腺癌病例进行回顾性分析。结果：外阴汗腺癌的发病年龄为４０～５８岁。７例患者的治疗为局部扩大切除或根治性外阴广泛切除，２例辅以术后放疗。７例中５例存活５年以上，其中１例曾在首次治疗后第７年、１１年２次复发并于第１７年死于肿瘤。２例存活＜５年，１例在术后２年半死于脑出血，１例因脊椎广泛转移在术后１年零９个月死亡。结论：外阴汗腺癌的预后较好，局部扩大切除或根治性外阴切除，是首选治疗方法。     

Reiter综合征--附2例报告及文献复习

目的探讨Reiter综合征的病因、临床特点、治疗方法. 方法报告2例并检索收集国内文献171例,共计173例Reiter综合征,对其临床资料进行分析讨论. 结果 173例Reiter综合征的男女之比为5.41,平均发病年龄为27±10岁.完全型126例,不完全型67例.继发干感染106例,HLA-B27阳性59/72例.控制感染后经消炎痛、泼昆松、甲氨蝶呤等治疗后,均能达到临床完全缓解. 结论 Reiter综合征是病原体感染诱发的自身免疫性疾病,预后良好.     

Juvenile Xanthogranuloma with Ocular Involvement

Abstract:   Juvenile xanthogranuloma is a benign histiocytic skin disorder encountered primarily in infancy and childhood. Approximately 0.4% of cases exhibit ocular manifestations, which can result in glaucoma and blindness. We present a case of a 7-month-old male with unilateral glaucoma associated with Juvenile xanthogranuloma, and emphasize the importance of an ocular screening in patients with Juvenile xanthogranuloma, especially those with periocular lesions.     

Risk behaviors and its association with caries activity and dental caries in Japanese children

The aim of this study was to assess risk behaviors and its association with caries activity and dental caries in Japanese children. The subjects were 392 young Japanese children who underwent dental health check-up at 18, 30 and 42 months of age. Oral examinations, Cariostat tests and dental health questionnaires were carried out at each time. The caries prevalence of children was 1.5% at 18 months, 9.9% at 30 months and 28.1% at 42 months. Caries activity based on the Cariostat scores of children was correlated with caries status (caries-free/carious) at 42 months. In children with caries during each examination period at 42 months, eating snacks while playing was ranked as the most important behavioral risk (P < 0.001), followed by breast-feeding (P < 0.01), non setting of time for snacks (P < 0.05) and frequency of snacks (P < 0.05) at 18 months old; non brushing by mother (P < 0.05) and eating snacks while playing (P < 0.05) were ranked highest at 30 months old. In addition, eating snacks while playing (P < 0.001) at 42 months old was the only a significant factor for children with caries. Caries activity and risk behaviors were associated with caries experience at different age periods of childhood.     

对我国目前麻风防治策略的思考

1949-2008年我国累计登记麻风患者48万余例,主要分布在北纬38°以南的东南沿海和长江流域省份.新中国成立后,经过积极防治,至20世纪80年代初,以氨苯砜单疗治愈的患者达31万余例;1986年在全国普遍推广WHO联合化疗方案,至2008年底全国累计接受联合化疗的麻风患者近8万例.     

应用腹直肌前鞘-腹外斜肌腱膜瓣修复先天性膀胱外翻

目的总结先天性膀胱外翻的修复,探讨腹直肌前鞘—腹外斜肌腱膜瓣及肌蒂阔筋膜张肌肌皮瓣在修复膀胱外翻中的应用。方法应用腹直肌前鞘—腹外斜肌腱膜形成的前鞘—腱膜瓣加局部皮瓣或同侧肌蒂阔筋膜张肌肌皮瓣修复先天性膀胱外翻及腹壁缺损,局部皮瓣修复尿道上裂,肌皮瓣的应用面积最大为10cm×8cm。结果临床应用12例,膀胱外翻及腹壁的修复均达到了良好效果,膀胱括约肌功能基本恢复,二次尿道紧缩后12例均能控制排尿。肌蒂阔筋膜张肌肌皮瓣部分坏死2例,游离植皮后痊愈。结论选择性地应用腹直肌前鞘及腹外斜肌腱膜形成前鞘—腱膜瓣加强腹壁,局部皮瓣转移或应用同侧肌蒂阔筋膜张肌肌皮瓣转移修复腹壁缺损能有效地修复先天性膀胱外翻,恢复膀胱颈部括约肌的连续性及尿道紧缩,能达到自主控制排尿的目的。     

The changes of the interspace angle after anterior correction and instrumentation in adolescent idiopathic scoliosis patients

Background  

In idiopathic scoliosis patients, after anterior spinal fusion and instrumentation, the discs (interspace angle) between the lowest instrumented vertebra (LIV) and the next caudal vertebra became more wedged. We reviewed these patients and analyzed the changes of the angle.