Chelerythrine chloride from Macleaya cordata induces growth inhibition and apoptosis in human gastric cancer BGC-823 cells

This study aimed to investigate the ability of chelerythrine chloride (CHE), the main active ingredient of Macleaya cordata, to induce apoptosis in human gastric cancer BGC-823 cells. The results demonstrate that CHE inhibits cell proliferation in a time- and dose-dependent manner with accompanying S phase arrest. It also induces apoptosis by a mechanism involving a reduction in the mitochondrial membrane potential, the release of cytochrome c, activation of caspase 3 and cleavage of poly-ADP-ribose polymerase. In addition, CHE-induced apoptosis is accompanied by down-regulation of Bcl-xl and Bcl-2 proteins with no change in the levels of Bax proteins. Taken together, the results support the development of CHE as a potentially useful anticancer drug for the treatment of gastric cancer.     

佛教思想对心理治疗观的影响

心理治疗的主要目的是减轻人们的心理痛苦。佛教如同任何宗教一样都有帮助人们减轻精神痛苦的作用。心理治疗源自西方的相对科学的体系,其理念重在以不同的方式帮助人们更好地适应环境,或更好地满足自己的需要。佛教中的理念引导人们应对心理痛苦的方式是接受放下,是一种人生态度。在心理治疗中渗入这一理念,会更有效地帮助来访者,至少在受佛教思想影响的中国文化背景下。本文将以心理治疗实践中的案例为例具体阐述佛教思想对心理治疗观的影响,并与心理动力学治疗和其他的心理治疗方法的一些理念进行比较。     

Changes in sputum cytology,airway inflammation and oxidative stress due to chronic inhalation of biomass smoke during cooking in premenopausal rural Indian women

To perform sputum analysis for verification of pulmonary changes in premenopausal rural Indian women chronically exposed to biomass smoke during cooking.Three consecutive morning sputum samples were collected from 196 women (median age 34 years) cooking with biomass and 149 age-matched control women cooking with cleaner fuel liquefied petroleum gas. Smears made on slides were stained with Papanicolaou and Perl's Prussian blue. Airway oxidative stress was estimated as reactive oxygen species (ROS) generation (by flow cytometry) and superoxide dismutase (SOD) level (by spectrophotometry) in sputum cells. Airway inflammation was measured as sputum levels of interleukin (IL)-6, -8 and tumor necrosis factor- alpha (TNF-α). Particulate matter of diameter less than 10 (PM₁₀) was measured using laser photometer while benzene exposure was monitored by measuring trans, trans-muconic acid (t,t-MA) in urine by HPLC-UV. Compared with control, sputum of biomass users contained more neutrophils, lymphocytes, eosinophils, alveolar macrophages, and showed presence of ciliocytophthoria, Charcot-Leyden crystals, Curschmann's spiral. ROS generation was increased by 2-fold while SOD was depleted by 31% in biomass users. They also had higher sputum levels of IL-6, -8 and TNF-α. Levels of PM₁₀ and t,t-MA were 2.9- and 5.8-times higher in biomass-using women. PM₁₀ and t,t-MA levels were positively associated with cellular changes in the sputum, markers of airway inflammation, and oxidative stress. Cooking with biomass alters sputum cytology, and increases airway inflammation and oxidative stress that might result in further amplification of the tissue damaging cascade in women chronically exposed to biomass smoke.     

Dissociation between exact and approximate addition in developmental dyslexia

Previous research has suggested that number sense and language are involved in number representation and calculation, in which number sense supports approximate arithmetic, and language permits exact enumeration and calculation. Meanwhile, individuals with dyslexia have a core deficit in phonological processing. Based on these findings, we thus hypothesized that children with dyslexia may exhibit exact calculation impairment while doing mental arithmetic. The reaction time and accuracy while doing exact and approximate addition with symbolic Arabic digits and non-symbolic visual arrays of dots were compared between typically developing children and children with dyslexia. Reaction time analyses did not reveal any differences across two groups of children, the accuracies, interestingly, revealed a distinction of approximation and exact addition across two groups of children. Specifically, two groups of children had no differences in approximation. Children with dyslexia, however, had significantly lower accuracy in exact addition in both symbolic and non-symbolic tasks than that of typically developing children. Moreover, linguistic performances were selectively associated with exact calculation across individuals. These results suggested that children with dyslexia have a mental arithmetic deficit specifically in the realm of exact calculation, while their approximation ability is relatively intact.     

One-dimensional molecular chains formed by Sierpiński triangles on Au(111)

One-dimensional molecular chains with Sierpiński triangles as building blocks were prepared on Au(111) and studied by low-temperature scanning tunneling microscopy. Fractal Sierpiński triangles were stabilized by the coordination interaction between 1,3-bi(4-pyridyl) benzene (BPyB) and Co atoms. At low coverages, Sierpiński triangles of high orders were observed. At high coverages, Sierpiński triangles formed two types of one-dimensional molecular chains. The high coverages and matching between molecular size and surface lattices are attributed to the formation of molecular chains.

One-dimensional molecular chains with Sierpiński triangles as building blocks were prepared on Au(111) and studied by low-temperature scanning tunneling microscopy.

From snowflakes to Saturn''s rings, self-similar fractals exist widely in nature and are of fundamental importance in mathematics, engineering, science, and arts. Fractal structures have been successfully synthesized in solutions and prepared on solid surfaces.^1–4 Various fractal Sierpiński triangles (STs) have been theoretically predicted via Monte Carlo simulations^5,6 and experimentally discovered by self-assembly or chemical reactions.^7–14 The arrays of STs are predicted to have particular optical, electric, and magnetic properties.^15,16 To measure them, high-order STs or regular patterns with STs as building blocks need to be constructed. Due to the limitation of kinetic growth, it is of high challenge to prepare high-order STs. As shown by the scanning tunneling microscopy (STM) experiments, the largest order of obtained STs is only 5.¹⁷ Preparation of ST arrays looks promising because various ways have been found to grow ordered patterns on surfaces. By a templating method, one-dimensional double chains of STs were prepared along the reconstructed rows on Au(100) at low molecular coverages.¹⁸ Without templates, large-scale ordered patterns of STs has not been prepared on Au(111) using the longer 4,4′′-dicyano-1,1′:3′,1′′-terphenyl (C3PC) molecule.Several factors are needed to be taken into account to construct regular crystalline arrays with STs as the building block on the three-fold Au(111) substrate. Firstly, inert surfaces and molecules with smaller sizes than C3PC are needed to get weak molecule–substrate interactions which allow molecular free diffusion. Secondly, using intermediately strong interactions as driving forces can make sure both the self-correction of defects formed during growth and sufficient molecular diffusion. Thirdly, the structure matching between molecules and surface lattices favors the formation of long-range ordered structures.Based on above considerations, 120° V-shaped BPyB molecules and Co atoms are used to build arrays of STs on Au(111). Low-temperature STM was used to characterize the formed structure at the single-molecule level. At low coverage, STs up to fourth order were observed in measurements. At high coverage, two types of large-scale arrays with STs as the repeated units were successfully prepared. As a comparison, the disordered structure formed by Co atoms and C3PC molecules at high molecular coverages was investigated.Experiments were performed by a Unisoku scanning tunneling microscope (STM) operating at 4.3 K with a base pressure of 10⁻¹⁰ torr. The single-crystalline Au(111) surface was cleaned by cycles of Ar⁺ sputtering and annealing. The cut Pt/Ir tip was first annealed in vacuum chamber and then dipped into Au(111) in a controlled way. The BPyB and C3PC molecules were thermally deposited onto the substrate from Ta boats, separately. The Co atoms were evaporated from a Knudsen cell onto Au(111). The ratio between Co and BPyB was slightly larger than 2 : 3 during deposition. The substrate was kept at room temperature when depositing molecules and Co atoms. All STM images were slightly smoothed using software WSxM.¹⁹After sublimating BPyB molecules and Co atoms onto Au(111) and annealing at 400 K for 10 minutes, isolated metal–organic coordinating STs were obtained in experiments at low molecular coverages. Here we denote STs of order n as BPyB–Co-ST-n or ST-n, where n ranges from 0 to 4. Fig. 1(a) and (b) present a BPyB–Co-ST-4 and its chemical structure, respectively. The node in STs is formed by one Co atom and three BPyB molecules through coordinating interaction, as shown in the upper right in Fig. 1(b). There are 81 Co atoms and 123 BPyB molecules in a BPyB–Co-ST-4. The iterative process of ST-n is demonstrated in Fig. 1(c) where the chemical structure of BPyB was simplified to a blue 120° polyline. In a BPyB–Co-ST-n, the number of BPyB molecules (N_BPyB) and Co atoms (N_Co) are: N_BPyB = 3(3ⁿ + 1)/2 and N_Co = 3ⁿ. Close inspection of the BPyB–Co-ST-4 reveals that they contain various pores of different sizes (Fig. 1(b)). The area of largest pore is around 44.6 nm². When increasing the coverage, BPyB molecules would cover the pores and the order n of STs would decrease.Open in a separate windowFig. 1Fractal phase formed at low molecular coverage. (a) STM image of a defect-free ST of 4 order; inset: coordinated trimer acting as basic recursive unit. The white arrow indicates 〈1$\bar{1}$0〉 direction. Scanning parameters: constant-height mode, V_B = 10 mV, I_t = 80 pA. (b) Structural model of the ST in (a). (c) Schematic models revealing the evolution process of fractal phase. Fig. 2(a) shows a large-scale STM image of Co–BPyB structures on Au(111), which was prepared by depositing BPyB molecules and Co atoms at high coverages and annealing at 400 K for 10 minutes. Partial STs are marked by triangles to guide the eye. Ordered packing of STs is found in the figure. One representative packing mode of STs is shown in Fig. 2(b). One-dimensional (1D) molecular ribbons consist of pairs of BPyB–Co-STs-2 marked by white dashed triangles. The high-resolution STM image and chemical structure are displayed in Fig. 2(c) and (d). Due to the space limitation, the marked pyridyl groups in apical molecules of STs are partially lifted from the Au(111) substrate (Fig. 2(d)). As a result, they appear darker than the rest parts of molecules in the STM image (Fig. 2(c)) because of a weaker coupling to the substrate. The molecular models in Fig. 2(e) are used to illustrate the hierarchically self-assembling process of 1D chains. The combination of a Co atom and three BPyB molecules leads to the formation of a threefold heterotactic node or ST-0. After the iterative process, the ST-1 and ST-2 can be obtained. Lateral translating the rhombic pair of STs-2 produces 1D molecular chains. Vertical packing molecular chains finally cause the formation of the experimentally observed chain array.Open in a separate windowFig. 2BPyB–Co-ST-2 arrays formed at high molecular coverage. (a) Large area of molecular ribbons formed by close-packed second order fractals. (b) Close-up image showing four compact rows of 1D molecular ribbons. (c) Details of two interlocking fractals (2 + 2) playing the role of initial repetitive unit. (d) Structural models corresponding to combination 2 + 2. (e) Hierarchical formation of compact 1D molecular ribbons originated from 1.6D fractal moieties. The white arrow points to 〈1$\bar{1}$0〉 direction. Scanning parameters: (a) constant-current mode, V_B = 1 V, I_t = 12 pA; (b) constant-current mode, V_B = 30 mV, I_t = 12 pA; (c) constant-height mode, V_B = 50 mV, I_t = 12 pA.Interestingly, another type of 1D structure was observed when annealing the sample at a lower temperature of 330 K at high coverages (Fig. 3(a)). These double chains are stabilized by mixed three- and four-fold coordination interactions. The structure marked by white dashed frames was magnified in Fig. 3(b). The high-resolution STM image reveals that the basic repetitive unit contains two BPyB–Co-STs-1 connected through a fourfold coordination node. The chemical structures are overlaid on the right side of the image to guide the eye. Similar to Fig. 2(e), the scheme in Fig. 3(c) is used to reveals the forming sequence of hierarchical chains shown in Fig. 3(a). Density Functional Theory (DFT, ESI^†) calculations reveal that the average binding energies of Co(BPyB)₃ and Co(BPyB)₄ are −1.63 and −1.47 eV, respectively. When annealing at 330 K, the insufficient molecular diffusion results in the metastable four-fold coordination. As expected, the surface structure changes to arrays of ribbons shown in Fig. 2(a) when further annealing the structure at 400 K to allow molecular free diffusion. Same to Co, both Fe and Ni can form STs with C3PC through metal–nitrogen coordinated bonds. Because the BPyB molecule is terminated by N atoms, we expect that similar surface structures should be obtained by replacing Co with Fe or Ni atoms.^10,11Open in a separate windowFig. 3BPyB–Co-ST-1 arrays formed at high molecular coverage. (a) STM image of 1D molecular ribbons formed by combination of 1 + 1. (b) Detailed image of one 1 + 1 ribbon superposed by models. The white arrow points to 〈1$\bar{1}$0〉 direction. Scanning parameters: constant-height mode, (a) V_B = 50 mV, I_t = 60 pA; (b) V_B = 10 mV, I_t = 70 pA; (c) schematic models indicate the formation of 1D molecular ribbons based on combination 1 + 1.To investigate the formation mechanism of 1D chains of STs, we repeated the experiments by replacing BPyB molecules by 4′′-dicyano-1,1′:3′,1′′-terphenyl (C3PC) molecules with other parameters unchanged. The longer C3PC molecule is terminated by cyano groups. Long-range ordered chains were not obtained at various annealing temperature and molecular coverages. Fig. 4(a) shows a representative STM image of Co–C3PC-STs on Au(111), where the longest chain is marked by a white rectangle. The magnified image (Fig. 4(b)) indicates that the molecular chain is composed of six Co–C3PC-STs. To be noted, two sides of the chain are not straight, as marked by white dashed curves.Open in a separate windowFig. 4C3PC–Co-STs formed at high molecular coverage. (a) Overview of irregular network formed by C3PC and Co coordination. The molecular model of C3PC is shown in the left inset. (b) Detailed STM image showing a strip of curved C3PC–Co-ST-2 array indicated by the white rectangle in (a). Both sides of the slightly distorted array are highlighted by dashed curves. Scanning parameters: (a) constant-current mode, V_B = 1.8 V, I_t = 17.5 pA; (b) constant-height mode, V_B = 10 mV, I_t = 15.4 pA.The C3PC molecule is slightly longer than BPyB. As a result, the degrees of commensurability between intermolecular lengths in STs and the lattice distance of Au(111) are different for these two molecules, which might account for their different growth behaviors. To quantitatively elucidate the relation between mismatching degrees and forming structures, K-maps with the undermentioned formula was calculated,K = |1 − |mi + nj|/d|.Here, i and j represent two basic vectors of Au(111) as indicated by black arrows in Fig. 5(a) and (b), with m and n as their integer factors. The lattice distances along these two directions are both 0.288 nm. In the formula, d corresponds to the theoretically calculated distance between two neighboring Co atoms, which equals 13.5 Å for Co–BPyB–Co and 16.9 Å for Co–C3PC–Co, respectively. K values at each (m; n) coordinate, representing the degree of commensurability, were calculated and displayed by squares with different brightness. A smaller K value means a better structural match between STs and Au(111). Four smallest K values are numbered in each K-map to highlight the favorable adsorption direction. For Co–BPyB-STs, the smallest mismatch value is 2.4% at the coordinate (4; 1). This explains well that the sides of Co–BPyB-STs are adsorbed along the 〈4$\bar{5}$1〉 directions in measurements. The adsorption energies of Co–BPyB–Co and Co–C3PC–Co motifs at representative coordinates have been calculated to demonstrate the validity of the K map (ESI^†). For Co–BPyB–Co, its adsorption energy at the coordinate of (4; 1) is 0.39 eV larger than that at the coordinate of (5; 0). The structures with smaller K values are more stable. It is clear that the calculated results are consistent with what the K-map indicates. For the longer Co–C3PC–Co motif, the difference of its adsorption energies at the coordinates of (4; 3) and (6; 0) is only around 0.05 eV. The experimentally observed adsorption directions of sides of the STs are along the 〈4$\bar{7}$3〉 directions and one side of an ST adsorbs nearly along the reconstruction row. For this kind of adsorption, the structural matching between STs and the herringbone reconstruction of Au(111) might account for the energy stabilization of STs. Along the 〈4$\bar{7}$3〉 directions, the K value is 3.7%, which is rather large and does not favors the formation the long-range ordered structures.Open in a separate windowFig. 5 K-Map of (a) BPyB–Co coordination and (b) C3PC–Co coordination. Each left panel corresponds to surface lattice models of Au(111) with the best (blue arrows) and second best (orange arrows) matching orientation; each right panel corresponds to relative gray-scale K maps.In summary, by using BPyB molecules and Co atoms, one-dimensional molecular chains with STs as building blocks were prepared on Au(111) and studied by low-temperature scanning tunneling microscopy. STs were stabilized by the coordination interaction between N atoms of BPyB and Co atoms. The chains were only obtained at high molecular coverages and isolated STs up to the fourth order were observed at low coverages. The structure matching between molecular size and surface lattices are attributed to the formation of molecular chains. As a comparison, long-range ordered chains were not obtained for C3PC molecules and Co atoms at various annealing temperature and molecular coverages. To be continued, it is of interest to investigate the surface structures by replacing Co with Fe or the comprehensive result by mixing the two different molecules and novel bicomponent structures are expected to emerge.²⁰