应用电化学测菌法检测变形链球菌的初步探讨

目的:探讨应用电化学测菌法检测变形链球菌的可行性。方法:利用本课题组前期建立的电化学测菌法检测变形链球菌,记录检测参数,并将电化学测菌法的检测结果与细菌培养结果进行比较。结果:①微流芯片样本通道内层流形成时间为(3.17±0.16)min,从进样到获得稳定电阻抗数据的时间为(10.20±0.16)min。②当变形链球菌浓度为104～109cells/mL时,细菌浓度C的对数与系统阻抗值Z呈良好的线性关系(R2=0.98)。③与细菌培养的检测结果相比,电化学测菌法获得的细菌数多,差异有统计学意义(P<0.05)。④变形链球菌浓度为105cells/mL时,电化学测菌法检测值与细菌培养法检测值之间的线性函数关系式为:y=0.94x+0.32E4(R2=0.95),两种方法的检测值高度正相关(P=0.001<0.01)。结论:电化学测菌法能为变形链球菌的快速检测提供新的思路。     

浓台氏液对口腔铸造用银铟合金的腐蚀性的初步研究

目的:通过体外浸泡实验观察口腔铸造用银铟合金与浓台氏液发生腐蚀反应后表面形貌及粗糙度的变化,考察不同腐蚀反应时间浓台氏液对银铟合金的腐蚀情况。方法:制作口腔修复铸造用银铟合金测试件,对照组不与浓台氏液接触,实验组浸入浓台氏液发生腐蚀反应,反应时间分别为5sec、10sec、15sec、30sec、1min、5min、10min、30min,以扫描电子显微镜观察试件表面形貌,再以粗糙度仪检测试件表面粗糙度,应用SPSS16.0统计软件对各组合金样本粗糙度值做统计学检验。结果:扫描电子显微镜结果显示:浓台氏液对银铟合金的腐蚀是在整个金属表面上均匀进行的,且腐蚀产物有随腐蚀时间的增加而增加的趋势。试件表面粗糙度检测结果显示:各试验组表面粗糙度值与电镜观察到的材料表面腐蚀程度一致,随腐蚀时间的增加而有增加的趋势。统计结果显示:5sec、10sec组与对照组两两相比粗糙度差异有统计学意义(P<0.05),而10min组、15sec、30sec、1min组、5min、10min组、30min组两两相比粗糙度均无显著差异(P>0.05)。结论:浓台氏液与银铟合金的腐蚀反应特征为均匀腐蚀。腐蚀产物和表面粗糙度有随腐蚀时间的增加而增加的趋势。表面粗糙度与腐蚀时间不呈线性增长关系,具体表现为表面粗糙度10min到达较高水平,后在30min内随时间的增加,浓台氏液对银铟合金表面的腐蚀基本稳定。     

综合医院口腔专科夜间急诊1206例临床分析与对策

目的:通过分析口腔专科夜间急诊病例的年龄、性别、就诊时间及病种,分析综合医院口腔科夜间急诊发病规律及特点.方法:收集我院2008.08-2011.07间,急诊病例1206例.按年龄分四个组,对各组就诊患者性别、就诊月份、病种进行统计,结果采用方差分析.结果:1、口腔专科夜间急诊病例的性别比例男∶女为1.32∶1;2、各年龄组就诊最高月份为7月,最低月份为2、3月;3、口腔专科夜间急诊就诊率高的病种分别为:颌面部外伤(38.64％),牙髓炎(13.76％),冠周炎(13.18％)和根尖周炎(8.96％).结论:针对综合医院口腔急诊患者的发病规律和特点,加强口腔急诊医疗救治的管理,提高口腔专科医师的临床急救技能,强化老年患者口腔疾病与全身健康关系的意识,熟悉和掌握临床急救知识,对提升口腔专科急诊医疗质量具有重要意义.     

Isolation and characterization of 113 polymorphic microsatellite loci for the Tibetan frog (Nanorana parkeri) using next generation sequencing

We developed and characterized 113 polymorphic microsatellites in the Tibetan frog (Nanorana parkeri) using 454 GS-FLX next generation sequencing technology. These loci were tested in 46 individuals from two N. parkeri populations from the Tibetan plateau. The average number of alleles per locus was 8.09 (range = 2–20). The average observed heterozygosities (H _O) per locus in the two populations were 0.58 (range = 0.04–1) and 0.29 (range = 0–0.87), respectively. These microsatellites will be useful for population and conservation genetics studies of N. parkeri and other closely related species.     

模拟失重及高+Gx对猴舌横纹肌细胞结构及HSP70表达的影响

目的探讨模拟失重及高+Gx对猴舌横纹肌组织结构和热休克蛋白70(heat shock protein 70,HSP70)表达的影响。方法 12只雄性猕猴随机分为4组,正常对照组(A)、30d模拟失重组(B),+13Gx/230s组(C)、30d模拟失重后超重组(D),其中D组根据过载峰值又分为+11Gx/270s(D1)、+13Gx/230s(D2)和+15Gx/200s(D3)。动物放血处死后取材,采用组织病理学和免疫组化的方法,观察猴舌横纹肌组织结构及HSP70的表达情况。结果 A组可见正常的舌横纹肌结构,其他组舌横纹肌细胞结构基本正常,偶见细胞间质稀疏,细胞排列紊乱以及横纹不清或消失。A组舌横纹肌细胞HSP70呈阴性表达,细胞核与细胞浆均无明显着色;B组、C组和D组舌横纹肌细胞胞核与胞浆均着色明显,呈阳性表达,较A组变化显著。HSP70免疫组化积分显示,D组比B组和C组变化显著(P<0.05),但D1、D2、D3无明显差别(P>0.05)。结论 30d模拟失重和高+13Gx/230s均可使猴舌横纹肌细胞产生应激反应,引起HSP70的表达增强,并可能伴有损伤。模拟失重与过载超重具有协同作用,两者可能加重了对舌横纹肌的影响。     

Anti-inflammation and antioxidant effect of Cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis, in middle cerebral artery occlusion-induced focal cerebral ischemia in rats

Cordymin is a peptide purified from the medicinal mushroom Cordyceps sinensis. The present study investigated the effects of Cordymin in prevention of focal cerebral ischemic/reperfusion (IR) injury. The right middle cerebral artery occlusion model was used in the study. The effects of Cordymin on mortality rate, neurobehavior, grip strength, glutathione content, lipid Peroxidation, glutathione peroxidase activity, glutathione reductase activity, catalase activity, Na⁺K⁺ATPase activity glutathione S transferase activity and on the regulation of C3 and C4 protein level, polymorphonuclear cells, interleukin-1β and tumor necrosis factor-α in a rat model were studied respectively. Treatment (orally) of Cordymin significantly boosted the defense mechanism against cerebral ischemia by increasing antioxidants activity related to lesion pathogenesis. Restoration of the antioxidant homeostasis in the brain after reperfusion may have helped the brain recover from ischemic injury. Moreover, Cordymin significantly inhibited infiltration of polymorphonuclear cells and IR-induced up-regulation of the brain production of C3 protein level, interleukin-1β and tumor necrosis factor-α. Cordymin significantly improved the outcome in rats after cerebral ischemia and reperfusion in terms of neurobehavioral function. Our findings suggest that cordymin have a neuroprotective effect in the ischemic brain, which is due to the inhibition of inflammation and increase of antioxidants activity related to lesion pathogenesis. Cordymin can be used as potential preventive agent against cerebral ischemia-reperfusion injury.     

发热待查75例临床分析

目的探讨不明原因发热(FUO)的病因和临床特征。方法回顾性分析2005年1月～2012年1月在我科住院且符合FUO诊断标准的75例患者的临床资料,总结了病因、诊断方法和临床表现。结果 75例患者中,68例(90.7%)最终确诊,感染性疾病22例(32.4%),其中结核病9例(40.9%)、传染性单核细胞增多症5例(22.7%);结缔组织病29例(42.6%),其中成人Still病17例(58.6%)、系统性红斑狼疮6例(20.7%);恶性肿瘤10例(14.7%),其中淋巴瘤6例(60.0%);其他疾病7例(10.3%),其中组织细胞坏死性淋巴结炎4例(57.1%)。结论 FUO涉及的病因众多,发病机制复杂,感染性疾病、结缔组织病仍是发热待查的主要原因,肿瘤性疾病在发热待查中也占相当的比例,临床工作中要开阔思路,全面搜集资料,以利于明确诊断。     

住院患者入院时血糖水平与医疗费用、疾病预后的相关研究

目的探讨住院患者人院时不同血糖水平与住院时间、医疗费用及疾病预后的关系。方法选取2009年11月至2011年7月期间人院的4868例患者,按入院24h内测得的血糖水平分为非高血糖组3429例、高血糖组1439例,后者包括糖尿病和应激性高血糖患者;根据患者年龄分为非老年组（年龄〈60岁）2532例和老年组（年龄≥60岁）2336例;其中278例冠心病患者再分为高血糖组120例和非高血糖组158例。各组间进行有关数据比较。结果高血糖组患者的住院天数、医疗费用和总病死率均显著高于非高血糖组（中位数住院日：15vs10d,P〈0．01;中位数医疗费用：14064．7vs8980．9元,P〈0．01;死亡率：2．92％vs0．61％,P〈0．01）。按年龄分组后,无论是非老年组还是老年组中糖尿病和应激性高血糖患者的医疗费用均明显高于非高血糖患者,住院日明显延长;应激性高血糖患者的医疗费用明显高于糖尿病患者;非老年组中应激性高血糖患者的死亡率明显高于非高血糖患者,但与糖尿病患者相比无明显差异,老年组糖尿病和应激性高血糖患者的死亡率无明显差异,但均明显高于非高血糖患者。冠心病患者中糖尿病和应激性高血糖患者与非高血糖患者的年龄无明显差异,前两组的医疗费用明显高于非高血糖组,住院日更长（分别为14,15和12d）、死亡率更高（分别为6．41％,7．14％和0．63％）。结论入院时高血糖水平预示患者有更高的医疗花费、更长的住院时间和更高的死亡率。     

Colletotrichum species associated with cultivated citrus in China

There have been considerable advances in the understanding of species concepts in the genus Colletotrichum. This has lead to the need to carry out fresh surveys of Colletotrichum species associated with important hosts. Colletotrichum species are associated with Citrus plants as saprobes, important pre-harvest and post-harvest pathogens, as well as endophytes. In this study, a total of 312 Colletotrichum strains were isolated from leaves, shoots and fruits of cultivated Citrus and Fortunella species with or without disease symptoms across the main citrus production areas in China. The morphology of all strains were studied and multilocus (ACT, TUB2, CAL, GAPDH, GS, ITS) phylogeny established. Strains were from four important species complexes of Colletotrichum, namely C. gloeosporioides species complex, C. boninense species complex, C. acutatum species complex and a final group including C. truncatum, which was rare on Citrus species. The species belonging to the C. gloeosporioides species complex comprised C. gloeosporioides and C. fructicola, the C. boninense complex comprised C. karstii and a new species C. citricola and the C. acutatum complex included a new species, C. citri. The ability of strains to cause anthracnose on citrus fruits was tested by inoculation and strains of Colletotrichum gloeosporioides, C. fructicola and C. truncatum were pathogenic.