松花江沿岸井水中微量元素含量及调查分析

目的:了解松花江沿岸井水中微量元素含量,并依国家卫生标准进行评价,为早期发现水污染提供科学依据。方法:沿黑龙江省境内的松花江流域选择采样点十三个,共458份水样,用原子吸收分光光度计检测微量元素的含量。结果:松花江流域井水中锌含量普遍偏低,所采集的458户水样中锌含量超出国家制定的饮用水卫生标准的仅有2份,有267份样品未检出锌,未检出率约为58.08%。井水中锰元素含量超过国家饮用水卫生标准规定量的现象较严重,采集的458份井水样品中,有158份样品锰超标,超标率为34.06%。松花江流域井水中镉含量均未超过国家制定的饮用水卫生标准。结论:松花江流域井水中锌含量偏低,锰含量超标严重,有害元素镉含量未超过国家标准。     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

黑龙江省在职卫生统计人员的现状分析与对策探讨

本文仅就黑龙江省专职卫生统计人员的现状进行分析，旨在摸清我省卫生统计队伍的总体情况，并提出相应的对策。     

鸡西市煤矿工人性格分析

为了解矿工的个性特征，采取随机整群抽样的方法，对鸡西市１６０名男性矿工用“艾森克个性问卷（ＥＰＱ）进行个性测定。本组矿工年龄平均为３０．５５岁（１７￣４９），文化程度以初中所占比例最大为６９．１２％。调查发现，在内外向个性（Ｅ）分布中，２０岁以上各年龄组Ｅ量表均值均高于我国常模，说明本组矿工多为外向性格；在情绪稳定性（Ｎ）分布中，各年龄组Ｎ量表均值与我国常模比较均无差别（Ｐ〉０．０５）；在精神质量     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

用高效液相色谱法测定维生素C的方法学研究

本文对维生素C(VC)测试探索用高效液相色谱法的内标法,得到了满意的效果。用荧光法对VC的测试条件作了优选,并对饮料中VC在保存期中的损失率,作了间隔时间的数次测试观察。     

基层领导干部在职称评定及聘后管理中的作用

建立完善的评价体系,真正实行优上劣下、竞争上岗的评审办法,大力提倡“五公开一监督”这一制约措施,有效地控制了人为因素的影响。通过公平竞争,使很多优秀中青年专业人才脱颖而出。 那么,在基层推荐过程中,是不是仅仅靠硬化条件打分,专家划圈就能得到预期结果呢?一个单位曾出现过这样一种情况:一位青年骨干,本人各方面条件均比较优秀,硬化条件打分时他排在10名的前几位。上面下达了4个副高职指标,本以为单位推荐是天经地义的事,可专家划圈时偏偏事与愿违,此人真的名落孙山了。本人和群众都大惑不解,甚至有的领导也