缺血性脑血管病与颈动脉粥样硬化及其危险因素的关系

目的探讨缺血性脑血管病(ICVD)与颈动脉粥样硬化及其危险因素的关系。方法对186例ICVD患者与194例非脑血管病患者和正常体检者(对照组)行颈部血管超声检查和血液生化检查;比较两组间的颈动脉硬化程度及脑卒中危险因素的差异。结果ICVD组年龄[(69±7)岁]和患有高血压(66.1%)、糖尿病(53.4%)、代谢综合征患者(44.6%)的比率非常明显高于对照组[(61±5)岁、48.8%、15.2%、12.9%](均P<0.001)。ICVD组颈动脉粥样硬化分级计分≥2分(斑块发生率)、≥3分(血管狭窄发生率)分别为69.3%、20.4%,明显高于对照组的33.5%和5.1%(均P<0.05)。结论颈动脉粥样硬化是ICVD的危险因素之一;各种危险因素的聚集对ICVD的发生起重要作用。     

A new haplogroup pattern displayed in Fujian Han in China

Human Y-chromosomal binary polymorphisms have been considered to preserve the paternal genetic legacy and provide evidence on human evolution and the genetic relationships among and demographic history of different populations. To reveal the genetic origin and immigration of the Fujian Han, 13 binary markers on the Y chromosome were used to screen Fujian Han by allele-specific polymerase chain reaction. The results indicated that the M₉G marker was highly prevalent (96.20%), suggesting a significant genetic drift. In addition, M₁₂₂C frequency was only 22.78%, and M₄₅A and M₁₀₃T were default. The distinctive haplogroup frequencies (H₁, H₅, and H_6/7/8) imply that the haplogroup pattern is a relatively ancestral and interim type. Received: October 13, 2001 / Accepted: December 3, 2001     

Safety and efficacy of ultrasound-guided low-pressure perfusion mini-percutaneous nephrolithotomy in children aged 1–7 years: a retrospective observational study

International Urology and Nephrology - This article analyzed the safety and efficacy of ultrasound-guided mini-percutaneous nephrolithotomy (MPCNL) with low-pressure perfusion for the treatment of...     

光猝灭法测定水杨酸醑中的水杨酸

目的：探索一种新的测定水杨酸醑中水杨酸的方法。方法：采用光猝灭法测定水杨酸醑中的水杨酸。结果：水杨酸浓度在 １～４０ μｇ／ｍ ｌ 范围内与 ｌｇ（ Ｆ０／ Ｆ）成线性,检测限为 １０ μｇ／ｍ ｌ,回收率为 １０２％ , Ｒ Ｓ Ｄ 为７８５％ ,与紫外分光光度法比较没有显著性差异（ Ｐ＞ ０．０５）。结论：本实验为在位、在线测定水杨酸奠定了基础。     

食管癌淋巴结转移的临床病理因素分析

目的:探讨食管癌淋巴结转移的临床病理相关因素.方法:对326例食管癌根治性手术病例进行统计,分析各主要临床病理改变与淋巴结转移的关系.结果:326例食管癌中淋巴结转移149例,转移率为45.7%.病变长度≤3cm、3.1～5cm、5.1～7cm、7.1～9cm和＞9cm的淋巴结转移率分别为28.6%、30.1%、51.4%、65.5%和73.7%.组织学类型中低分化、中分化、高分化鳞癌,鳞腺癌、腺癌的淋巴结转移率分别为87.0%、34.0%、14.3%、57.1%和62.5%.浸润深度T1、T2、T3、T4的淋巴结转移率分别为13.3%、29.9%、47.8%和92.6%.以上三种因素间比较均有显著性差异(P＜0.001).淋巴结转移率与性别、年龄、民族、肿瘤部位和病理大体类型的关系不大.病变长度与浸润深度呈正相关.结论:肿瘤越大(长),浸润越深,分化程度越低,易发生淋巴结转移.     

超高速细胞分选平台结合cDNA microarray技术筛查宫颈癌细胞潜在分子标志物

目的：通过超高速细胞分选平台结合cDNA microarray技术,筛查宫颈癌细胞可能潜在的分子标志物。方法：采用MoFlo XDP型超高速细胞分选平台纯化细胞膜表面表达CD38和不表达CD38的宫颈癌细胞,利用RNAlater技术得到cDNA microarray实验所需RNA,然后进行基因芯片分析。结果：利用MoFlo XDP型超高速细胞分选平台可以获得纯度为99.0%以上的CD38阳性表达宫颈癌细胞。结论：cDNA microarray分析发现了RORA、PLIN4、AUTS2、IFITM1等宫颈癌细胞潜在分子标志物,为宫颈癌研究提供了新的技术方法。     

儿童哮喘过敏原检测及临床意义

目的 探讨皮肤过敏原点刺试验、血清过敏原检测在儿童哮喘病因诊断、治疗的临床意义。方法 应用粉尘螨及屋尘螨过敏原对121例哮喘患儿及111例对照组儿童进行皮肤点刺试验,其中59例哮喘患儿应用免疫条带印记法进行血清过敏原检测。结果 ①121例哮喘患儿尘螨过敏原皮肤点刺试验总阳性率为28．10％,高于对照组（P〈0．05）。②粉尘螨过敏原阳性33例（27．27％）;屋尘螨过敏原阳性32例（26．45％）。③粉尘螨及屋尘螨过敏原皮肤点刺试验结果与哮喘急性发作严重程度有关。④59例哮喘患儿血清过敏原检测阳性46例（77．97％）,其中猫毛26例（44．07％）。狗毛23例（38．98％）,粉尘螨13例（22．03％）,屋尘螨12例（20.34％）,海鲜组合10例（16．95％）。⑤皮肤点刺试验与血清过敏原检测的尘螨过敏原检出率,差异无统计学意义。结论 皮肤点刺试验可作为儿童哮喘过敏原检测优先选用的方法。     

Calcineurin-NFAT signaling is involved in phenylephrine-induced vascular smooth muscle cell proliferation

Aim： Catecholamine-induced vascular smooth muscle cell （VSMC） proliferation is one of the major events in the pathogenesis of atherosclerosis and vascular remodeling. The calcineurin-NFAT pathway plays a role in regulating growth and differentiation in various cell types. We investigated whether the calcineurin-NFAT pathway was involved in the regulation of phenylephrine-induced VSMC proliferation. Methods： Proliferation of VSMC was measured using an MTT assay and cell counts. Localization of NFATcl was detected by immunofluorescence staining. NFATcl-DNA binding was determined by EMSA and luciferase activity analyses. NFATcl and calcineurin levels were assayed by immunoprecipitation. Results： Phenylephrine （PE, an α1-adrenoceptor agonist） increased VSMC proliferation and cell number. Prazosin （an α1-adrenoceptor antagonist）, cyclosporin A （CsA, an inhibitor of calcineurin） and chelerythrine （an inhibitor of PKC） decreased PE-induced proliferation and cell number. Additional treatment of VSMC with CsA or chelerythrine further inhibited proliferation and cell number in the chelerythrine-pretreatment group and the CsA-pretreatment group. CsA and chelerythrine alone had no effect on either absorbance or cell number. CsA decreased PE-induced calcineurin levels and activity. NFATcl was translocated from the cytoplasm to the nucleus upon treatment with PE. This translocation was reversed by CsA. CsA decreased the PE-induced NFATcl level in the nucleus. PE increased NFAT＇s DNA binding activity and NFAT-dependent reporter gene expression. CsA blocked these effects. Conclusion： CsA partially suppresses PE-induced VSMC proliferation by inhibiting calcineurin activity and NFATcl nuclear translocation. The calcineurin-NFATcl pathway is involved in the hyperplastic growth of VSMC induced by phenylephrine.