Development a nomogram to predict fertilisation rate of infertile males with borderline semen by using semen parameters combined with AMH and INHB

The sperm quality of some males is in a critical state, making it hard for clinicians to choose the suitable fertilisation methods. This study aimed to develop an intelligent nomogram for predicting fertilisation rate of infertile males with borderline semen. 160 males underwent in vitro fertilisation (IVF), 58 of whom received rescue ICSI (R-ICSI) due to fertilisation failure (fertilisation rate of IVF ≤30%). A least absolute shrinkage and selection operator (LASSO) regression analysis identified sperm concentration, progressively motile spermatozoa (PMS), seminal plasma anti-Müllerian hormone (spAMH), seminal plasma inhibin (spINHB), serum AMH (serAMH) and serum INHB (serINHB) as significant predictors. The nomogram was plotted by multivariable logistic regression. This nomogram-illustrated model showed good discrimination, calibration and clinical value. The area under the receiver operating characteristic curve (AUC) of the nomogram was 0.762 (p < .001). Calibration curve and Hosmer–Lemeshow test (p = .5261) showed good consistency between the predictions of the nomogram and the actual observations, and decision curve analysis showed that the nomogram was clinically useful. This nomogram may be useful in predicting fertilisation rate, mainly focused on new biomarkers, INHB and AMH. It could assist clinicians and laboratory technicians select appropriate fertilisation methods (IVF or ICSI) for male patients with borderline semen.     

扎里奴思方对脑缺血再灌注损伤后大鼠脑内NGF表达的影响

目的：观察扎里奴思方对脑缺血再灌注损伤后大鼠脑内神经生长因子（NGF）表达的影响。方法：线栓法制备局灶性脑缺血再灌注大鼠模型。实验动物随机分为假手术组、模型组、扎里奴思方组、尼莫地平组。取材前进行神经功能评分。术后各组分别在1,3,7,14 d各个时间点取材,HE染色法观察大鼠脑组织病理学改变;免疫组织化学法测定大鼠脑内NGF 的表达。结果：模型组大鼠神经功能评分降低,神经元数量减少,大小不均,病理损伤显著;与模型组比较,尼莫地平和扎里奴思方组神经评分增高,神经元数量增多,脑组织病理损伤减轻明显,NGF表达增高（P<0.01,P<0.05）;与尼莫地平组比较,扎里奴思方14 d组神经功能评分和NGF表达明显升高（P<0.05）。结论：扎里奴思方可对局灶性脑缺血再灌注大鼠脑组织神经元起保护作用,扎里奴思方14天组的脑保护作用更强,其脑保护作用机制可能与调节大鼠脑组织 NGF 表达有关。     

Association Between a Body Shape Index and Subclinical Carotid Atherosclerosis in Population Free of Cardiovascular and Cerebrovascular Diseases

Aim: We used a dataset from a cross-sectional survey conducted in China to determine which of the anthropometric indices of obesity are important in terms of carotid atherosclerosis free of cardiovascular and cerebrovascular diseases. Methods: A total of 5,245 participants who were volunteering for carotid ultrasound unit in this cross-sectional survey were included in the present analysis. All subjects were free of angina, myocardial infarction, heart failure and stroke, and cancer. A low-risk subgroup was defined as people free of hypertension, diabetes, and hyperlipidemia. All analyses based on logistic regression were gender-specific. Results: The present study consisted of 2,501 males and 2,744 females, with 776 (31.03%) diagnosed as carotid artery plaque in males and 550 (20.04%) in females. Univariable analyses in unadjusted logistic model showed significant associations between disease presence and all central obesity indices. After adjusting for more variables, only a body shape index (ABSI) was associated with the presence of disease in both males and females. Moreover, stepwise regression approaches revealed that ABSI was always an independent determinant of the presence of subclinical carotid plaque. Multiple regression shows a linear and significant increase in the prevalence of atherosclerosis in males and females as ABSI decile levels increased. Similar results were obtained when the association between ABSI and carotid plaque was studied in this low-risk subgroup. Conclusions: ABSI, as a novel anthropometric indicator compared with traditional indices, was found to have a closer relationship with subclinical carotid atherosclerosis, even in populations free of hypertension, diabetes, and hyperlipidemia.     

电纺参数对PLGA/HA复合支架纤维形貌和直径的影响

目的通过静电纺丝的方法制备PLGA/HA复合支架,探讨电纺参数对复合支架纤维形貌和直径的影响。方法以三氯甲烷和N,N-二甲基甲酰胺为混合溶剂制备PLGA/HA纺丝液,通过调节PLGA的浓度、电压、接收距离,制备具有不同表面形貌的PLGA/HA复合纤维,采用SEM观察PLGA的浓度、电压、接收距离对纤维形貌和直径的影响。结果复合纤维的直径随PLGA浓度的增加而增加;随电压的增加而增加;随接收距离的增加先减小后增加。结论制备PLGA/HA复合支架较合适的电纺参数为：PLGA浓度25%,电压20KV,接收距离15cm。静电纺丝法制得的PLGA/HA复合支架有可能作为骨组织再生的支架在组织工程领域发挥作用。     

Qiangli Wuhu mixture alleviates LPS-induced pneumonia by inhibiting the TLR4/NF-κB/NLRP3 pathway: a study based on network pharmacology

ContextQiangli Wuhu (QLWH) mixture is a concoction approved and registered by Ningxia Medical Products Administration. It has therapeutic effects on various types of pneumonia.ObjectiveTo clarify the mechanisms of QLWH in treating pneumonia.Materials and methodsThe potential targets of QLWH in the treatment of pneumonia were predicted by network pharmacology. Male, Institute of Cancer Research (ICR) mice were randomly divided into five groups of 12 mice, control, vehicle, QLWH (10 and 20 mg/kg) and dexamethasone (DXM), and orally treated twice daily with normal saline, QLWH or DXM. The pneumonia model was established by tracheal instillation of lipopolysaccharide (LPS). After treatment five days, ELISA, H&E staining and Western blot were used to investigate protective effects of QLWH.ResultsNine hundred and ninety-four active ingredients were found through network pharmacology, corresponding to 135 targets for the treatment of pneumonia; compared to the vehicle group, QLWH (10 and 20 mg/kg) significantly decreased the levels of TNF-α (14.3% and 28.8%), IL-1β (23.9% and 42.8%) and IL-6 (13.2% and 16.1%), increased the levels of IL-10 (134.3% and 172.9%); in terms of mechanism, QLWH down-regulated TLR4/NF-κB/NLRP3 axis related proteins in lung tissue of pneumonia model mice (p < 0.05).Discussion and conclusionsThis study combined network pharmacology and animal experiments, providing effective evidence for the clinical promotion of QLWH. Meanwhile, it is of significance for further development.     

黄芩苷对流感病毒性肺炎小鼠肺组织内皮素及其受体的作用

目的:探讨黄芩苷对流感病毒性肺炎小鼠肺组织血清内皮素(ET-1)及其受体的作用。方法:制备甲型流感病毒(H1N1)性肺炎小鼠模型,用不同剂量黄芩苷溶液灌胃治疗后,观察小鼠生活状态,计算平均生存日及生命延长率;制作肺组织切片,观察HE染色后的病理变化;采用荧光定量RT-PCR测定肺组织ETAR、ETBR mRNA表达,采用ELISA方法检测血清中ET-1含量。结果:黄芩苷中、高剂量组小鼠平均生存时间均有不同程度的延长;肺组织ETAR和ETBR mRNA表达显著降低(P<0.01);血清中ET-1蛋白表达明显降低(P<0.05)。结论:黄芩苷能够减轻流感病毒感染致肺损伤可能与其通过下调内皮素受体和ET-1表达,促进肺部血液循环有关。     

PBL联合CBL教学法在急诊住院医师规范化培训教学中的应用

目的 探讨PBL联合CBL教学法在急诊住院医师规范化培训教学中的应用效果。方法 选择2019年3—6月在本科室参加住院医师规范化培训的80名学员,随机分为两组,各40名。其中对照组采用PBL教学方法,观察组采用PBL联合CBL教学方法授课。带教结束后,采用DOPS评分、Mini-CEX评分、技能操作和学员满意度调查问卷评估两组学员的教学成果。结果 与对照组相比,观察组学员DOPS各项指标及总分、教学满意度、Mini-CEX考核评分、急救技能操作成绩均高于对照组,且差异均有统计学意义（P <0.05）。结论 PBL联合CBL教学法在急诊住培教学中有助于提高学员的学习能力,提升急诊临床教学质量。     

LIG4mRNA与XRCC4mRNA在宫颈癌中的表达及临床意义

目的探讨宫颈癌组织中DNA连接酶4(LIG4)及X射线修复交叉互补基因4(XRCC4)的mRNA表达水平及临床意义。方法应用实时荧光定量RT-PCR技术检测61例宫颈癌和20例正常宫颈组织中LIG4和XRCC4的mRNA表达水平,分析两种基因的表达与宫颈癌分期、分化程度及盆腔淋巴结转移之间的关系。结果 LIG4 mRNA和XRCC4mRNA在宫颈癌组织及正常宫颈组织中的表达分别为(2.88±1.97)、(5.02±1.87)和(2.16±0.99)、(3.72±1.36),差异有统计学意义,P值分别为0.00和0.00;宫颈癌患者盆腔淋巴结有转移组和无转移组中的表达分别为(0.61±0.17)、(0.41±0.28)和(0.80±0.31)、(0.58±0.26),差异有统计学意义,P值分别为0.00和0.01;宫颈癌低分化组和中高分化组中的表达分别为(1.81±0.99)和(2.03±1.25)、(1.50±0.76)和(1.45±0.57),差异无统计学意义(P>0.05)。LIG4 mRNA在Ⅱ期和Ⅲ期宫颈癌组织中的表达分别为(0.71±0.34)和(0.96±0.36),差异有统计学意义,P=0.01。LIG4 mRNA与XRCC4mRNA在宫颈癌组织中表达呈正相关(r=0.38,P=0.00)。结论 LIG4 mRNA和XRCC4 mRNA在宫颈癌组织中的异常表达与宫颈癌的发展及盆腔淋巴结转移密切相关。     

枸杞多糖对去势雌性大鼠骨质疏松影响的研究

目的研究枸杞多糖（LBP）对去势雌性大鼠骨质疏松的影响。方法选用2月龄成年雌性SD实验大鼠50只,随机分为假手术组、去势＋生理盐水组、去势＋枸杞多糖高剂量组、去势＋枸杞多糖低剂量组、去势＋己烯雌酚组等5组,每组10只。术后各组常规饲养10周后给药干预,12周后行股骨干重测量、骨矿密度检查并观察骨的形态学改变。结果与去势＋生理盐水组相比,去势＋枸杞多糖高剂量组、去势＋低剂量组均能增加去势雌性SD大鼠股骨干重、骨密度值,两组骨小梁结构较完整、连续性较好、吸收陷窝少。结论枸杞多糖对成年去势雌性大鼠骨质疏松有明显的骨质改善作用。