时空统计方法

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The pyrimido-pyrimidine derivatives RA 233 and RX-RA 85 affect growth and cytoskeletal organization ofrat mammary adenocarcinoma cells

The pyrimido-pyrimidine derivatives RA 233 and RX-RA 85, which are potent inhibitors of platelet and tumor phosphodiesterase, were developed as antitumor agents. When tested by us, these drugs were cytostatic at low concentrations and produced dramatic changes in cell shape and organization of cytoskeletal structures in cultured MTF7 cells derived from the rat 13762NF mammary adenocarcinoma. At high concentrations (up to 600 μg/ml) RA 233 was cytostatic but not cytotoxic to MTF7 cells during a 24 hr incubation in vitro, whereas RX-RA 85 was cytotoxic at concentrations above 4 μg/ml. These drugs caused MTF7 cells to elongate and form numerous vacuoles, which surrounded the cell nucleus. Treatment of MTF7 cells with RA 233 or RX-RA 85 enhanced microtubular organization concomitant with a decrease in microfilament organization. In contrast, treatment of MTF7 cells with 1 mM dibutyryl cAMP resulted in an enhanced organization of microtubules but had no effect on microfilament organization. Previous studies that RA 233 and RX-RA 85 increase cAMP levels in 2 other cell clones of rat 13762NF mammary adenocarcinoma by inhibiting phosphodiesterases. However, additional sites of drug action should also be considered based on the effect of these drugs on microfilament systems and cell vacuoles.     

SF-36量表应用于城市化居民生命质量评价的信度和效度分析

目的 评价SF-36量表用于城市化居民生命质量评价的信度和效度,为选择合适的健康测量工具提供参考。方法 采用多阶段整群系统抽样方法,使用SF-36量表进行入户调查,利用Spearman-Brown系数和Cronbach''s α 系数评价量表分半信度和内部一致性信度,集合效度实验和区分效度实验成功率评价量表集合效度和区分效度,采用相关分析和非参数检验以自评健康状况为标准进行标准关联效度分析,结构效度评价采用基于结构方程模型的验证性因子分析。结果 SF-36量表应用于城市化居民生命质量评价具有良好的分半信度(R=0.94)和内部一致性信度(除"躯体疼痛"和"精力"维度外,Cronbach''s α 系数为0.70~0.91),量表集合效度(定标成功率为88.57%)、区分效度(定标成功率为90.61%)和准则效度(γ_s=0.56,评分与自评健康状况相一致)良好,二阶验证性因子分析模型对数据拟合度较差(拟合优度指数为0.721,调整拟合优度指数为0.682,比较拟合指数为0.731,残差均方和平方根为0.084,近似误差均方为0.098),提示结构效度欠佳。结论 SF-36量表用于城市化居民生存质量评价具有良好的信度、集合效度、区分效度和标准关联效度,但结构效度欠佳,建议在实际应用中对相应条目进行调整。     

HIV现场快速检测在浴池男男性接触人群中的应用

目的通过现场快速检测方法在浴池MSM人群艾滋病性病干预检测的应用,建立和完善可行的干预流程,探讨浴池MSM人群初筛阳性者高流失情况的解决方法。方法按自愿原则分2组,1组为现场快检组,在浴池对MSM人群开展快速检测HIV抗体;另1组为常规检测组,现场采血。两组均进行人口学、性行为学特征问卷调查和血清常规HIV、梅毒的检测。结果 180名调查对象中,87名自愿参加现场快检,发现初筛HIV抗体阳性10名,感染率11.49%;93名参加常规检测,发现初筛HIV抗体阳性15名,感染率16.13%,现场快检与常规检测HIV阳性发现率差异无统计学意义(P>0.05)。未婚人员参加现场快检较常规检测比例高(P<0.05)。检测对象结果整体告知92.78%,常规检测与现场快检结果告知情况差异无统计学意义(P>0.05)。HIV初筛阳性者常规检测追踪到位率60.00%,失访率40.00%;现场快检追踪到位率100.00%,现场快检失访率明显低于常规检测(P<0.05)。结论现场快速检测HIV抗体方法在浴室MSM人群干预中具有很强的实用性,值得推广使用。