Association of angiotensin Ⅱ type 1 receptor gene polymorphism with essential hypertension

Objective To determine whether the polymorphism A1166C in the angiotensin Ⅱ type 1 receptor (AT1R) gene is associated with essential hypertension.Methods A case-control study was carried out using 125 hypertensive and 103 normotensive subjects. The A→C variant at position 1166 (A1166C) of t he AT1R gene was identified by polymerase chain reaction (PCR) and PCR/ restriction fragment length polymorphism (PCR/RFLP) analysis. The digestion products were separated on 2% agarose gels and visualized with ethid ium bromide under ultraviolet ray.Results The differences in C1166 allele frequency and in the AC genotype dist ribution of the AT1R gene between the hypertensive and normotensive groups were statistically significant (C allele: 0.092 vs 0.034, χ(2) =6.1 86, P<0.05; AC genotype: 0.184 vs 0.068, χ(2) =6.654, P<0.05).Conclusion The AC genotype is associated with essential hypertension, and the C a llele may be a marker for predisposition to hypertension in Chinese H an population.     

Allogeneic peripheral blood stem cell transplantation for leukemia

Objective To observe engraftment kinetics, the incidence and severity of graft-versus-ho st disease (GVHD), and clinical outcome on 40 recipients undergoing allogeneic p eripheral blood stem cell transplantation (allo-PBSCT).Methods From June 1997 to May 1999, forty leukemia patients with a median age of 35 year s underwent allo-PBSCT. PBSC were mobilized with G-CSF at a dose of 5 μg/kg s.c. every 12 hours for 5 days. A median of 7.7 (2.0-16.8)×10 (6) CD34(+) cells/kg was infused into the recipients. Busulfan-cyclophosphamid e (BU-CY) was used as the conditioning regimen. All patients received cyclospo rine A and either methotrexate (n=34) or methylprednisolone (n=6) for GVHD p rophylaxis. Results Engraftment of neutrophils and platelets was achieved at a median of 13 days (9 -28 days) and 12 days (7-60 days) respectively. Patients receiving ≥4×10 (6) CD34(+) cells/kg or given G-CSF post transplant had significantl y accelerated neutrophil and platelet engraftment. Acute GVHD occurred in 17 of 40 patients (42.5%), with grade Ⅱ-Ⅳ acute GVHD in 10 patients (25%). Chron ic GVHD developed in 21 (9 extensive, 12 limited) out of 30 evaluable patients ( 21/30, 70%) with a median follow up of 380 days (180-900 days). Transplan t related mortality was 17.5% and the relapse rate was 10%. The probability of leukemia free survival at 3 years was 72.5%.Conclusion Allo-PBSCT can provide rapid hematopoietic reconstitution without an increased incidence of acute GVHD, but may be associated with a high risk of chronic GVHD .     

Clinical significance of elevated serum sICAM-1 in patients with nasopharyngeal carcinoma]

OBJECTIVE: To study the expression of serum sICAM-1(soluble intercellular adhesion molecule-1) and tissue ICAM-1 in nasopharyngeal carcinoma and its clinical significance. METHODS: Expression of ICAM-1 in frozen sections of 20 normal nasopharyngeal mucosa and 54 nasopharyngeal carcinoma samples were studied with immunohistochemical technique, and levels of serum sICAM-1 in 54 patients with nasopharyngeal carcinoma (NPC group) and 32 healthy adults (normal group) by ELISA. RESULTS: Positive expression (45/54) of tissue ICAM-1 and levels (2,044.48 +/- 640.05) microgram/L of serum sICAM-1 in NPC group were significantly higher than that of 2/20 and (313.70 +/- 114.98) microgram/L in normal group (P < 0.01, respectively), and there was a positive relationship between the expression of tissue ICAM-1 and levels of serum sICAM-1 in nasopharyngeal carcinoma(P < 0.05). The levels (2,177.31 +/- 642.13) microgram/L of serum sICAM-1 in patients with III-IV stages were higher than those (1,847.25 +/- 308.15) microgram/L in I-II stages (P < 0.05), and those (1,678.89 +/- 685.01) microgram/L in patients with no lymph node metastasis lower than that (2,134.34 +/- 609.60) microgram/L in lymph node metastasis (P < 0.05). The levels of serum sICAM-1 decreased significantly from (2,211.42 +/- 717.59) microgram/L to (926.55 +/- 207.72) microgram/L in disappearance of tumor and metastatic lymph node after radiotherapy (P < 0.01). CONCLUSION: sICAM-1 might be involved in the cellular immunopathogenesis of nasopharyngeal carcinoma, and the measurement of the serum sICAM-1 may reflect ability of neoplasm cell in freedom from host immunological surveillance. Dynamic measurement of serum sICAM-1 may be a useful objective index to evaluate NPC prognosis and curative effects.     

Human monocyte-derived dendritic cells expressing both chemotactic cytokines IL-8, MCP-1, RANTES and their receptors, and their selective migration to these chemokines

Ｄｅｎｄｒｉｔｉｃｃｅｌｌｓ(ＤＣｓ)ａｒｅｔｈｅｍｏｓｔｐｏｔｅｎｔａｎｔｉｇｅｎｐｒｅｓｅｎｔｉｎｇｃｅｌｌｓａｎｄｓｐｅｃｉａｌｉｚｅｉｎｓｔｉｍｕｌａｔｉｎｇｒｅｓｔｉｎｇＴｃｅｌｌｓａｎｄｉｎｉｔｉａｔｉｎｇＴｃｅｌｌｄｅｐｅｎｄｅｎｔｉｍｍｕｎｅｒｅｓｐｏｎｓｅｓ1ＤＣｐｒｅｃｕｒｓｏｒｓｏｒｉｇｉｎａｔｅｉｎｔｈｅｂｏｎｅｍａｒｒｏｗａｎｄｓｕｂｓｅｑｕｅｎｔｌｙｍｉｇｒａｔｅｉｎｔｏｐｅｒｉｐｈｅｒａｌｔｉｓｓｕｅｓａｎｄｐｒｉｍａｒｙｌｙｍｐｈｏｉｄｏｒｇａｎｓｗｈｅｒｅｔｈｅｙ…     

含氯含碘及戊二醛类高效消毒剂毒性探讨

目的探讨高效消毒剂的毒性作用。方法选择某品牌的三种消毒剂,参照《消毒技术规范》中的试验方法进行了实验。结果①某品牌消毒片（含氯）小鼠经口ＬＤ５０为２２２８ｍｇ／ｋｇ,属低毒类,戊二醛和复合碘为实际无毒类;②消毒片和戊二醛一次皮肤刺激、复合碘一次眼刺激和多次皮肤刺激试验均无明显刺激性;③三种消毒剂小鼠骨髓细胞微核试验均为阴性,致突变性检测结果为非染色体断裂剂。结论三种消毒剂急性毒性和对皮肤粘膜的刺激作用是可以接受的     

磁处理黄酒对实验性高脂血症大鼠血脂水平的影响

目的　研究磁处理黄酒对大鼠血脂水平影响,探索食物控制血脂新途径。方法　采用卫生部《保健食品功能学评价程序和检验方法》中调节血脂作用检验方法高血脂模型法进行本次研究。结果　高剂量组（７５０ｍ ｌ／ｋｇ）受试物能显著降低高脂血症大鼠血清胆固醇（ Ｔ Ｃ）和血清甘油三酯（ Ｔ Ｇ）的含量。结论　磁处理黄酒具有调节血脂作用,适量饮用对防止心血管疾病可能具有良好的保健作用。     

带泵肝胆肿瘤病人再手术时泵的处理

皮下埋藏全植入式抗癌药泵是一种行区域性化疗的装置,术后定期经泵注药,其特点是提高已切除或晚期不能切除瘤灶区域的药物浓度,减少全身副反应,给药方便,自１９９３年８月至１０月完成肝胆恶性肿瘤中埋泵２０５例,其中８例肿瘤复发再次手术,本文介绍再手术时动脉静脉泵和导管处理的经验教训以及泵体的合理安放,避免埋泵的失败。     

矽肺患者的血液流变状况及其临床意义

目的　探讨矽肺与血液流变学的关系及其临床意义。方法　检测了８５ 例男性矽肺患者、４５ 例男性慢性支气管炎患者及１００ 例健康男性对照者的血液流变学各项指标值。结果　与对照组和慢性支气管炎组比较,矽肺组的红细胞压积、切变率为４ ｓ－１ ～２００ ｓ－１ 时的全血表观黏度、血浆黏度、红细胞电泳时间、血小板电泳时间平均值均显著升高;不同病情矽肺组、不同肺功能状况矽肺组上述检测值的平均值均随病情加重、肺功能下降而升高,并均显著高于对照组。逐步回归发现,矽肺患者病情与红细胞压积、４ ｓ－１和２００ ｓ－１ 切变率时的全血表观黏度、红细胞电泳时间和血小板电泳时间相关最密切,而肺功能状况和病程却均与红细胞压积、４ ｓ－１ 和２００ ｓ－１切变率时的全血表观黏度、血浆黏度、红细胞电泳时间和血小板电泳时间相关最密切。结论　矽肺患者的血液呈高黏滞状况,极易并发多种疾病。     

洁康手术消毒剂杀菌性能和毒性观察

目的　为了解洁康手术消毒剂的杀菌活性和使用安全性。方法　按照１９９７ 年卫生部“消毒技术规范”（ 试行） 版规定的方法,对其杀灭金黄色葡萄球菌、大肠杆菌和手部自然菌进行了实验室和现场试验。结果　含醋酸氯己啶８０ｍｇ／Ｌ样液作用１ｍｉｎ,对悬液中大肠杆菌、金黄色葡萄球菌的平均杀灭率达９９ ．９９ ％ ,作用３ｍｉｎ 均达１００ ％ 。样品原液作用３ｍｉｎ 对手指皮肤表面污染的大肠杆菌的平均灭除率为９９ ．９８ ％ ,对自然菌的平均灭除率为９８ ．２１ ％ ,且消毒后均达到《医院消毒卫生标准》中Ⅰ、Ⅱ类环境医护人员手细菌菌落总数卫生标准要求。样品经５４ ℃恒温存放１４天,醋酸氯己啶的降解率为２ ．５４ ％ ,毒性检测结果表明无毒、对皮肤无刺激。和碘伏外科洗手消毒比较结果表明,其外科洗手消毒效果优于碘伏。结论　在使用浓度和作用时间下,可有效杀灭细菌,且手部皮肤表面残留菌数达到医院Ⅰ、Ⅱ类环境医护人员手细菌总数卫生标准要求     

季铵盐消毒剂中检出一株洋葱假单胞菌

目的　观察从复合季铵盐消毒剂中分离得到的洋葱假单胞菌对常用消毒剂的抗力。方法　以常用的氯和戊二醛消毒剂对其进行杀菌试验,并与对大肠杆菌和金黄色葡萄球菌相应的杀菌效果进行比较,以除菌后污染样液对大肠杆菌、金黄色葡萄球菌进行了杀菌试验和ＨＢｓＡｇ 抗原性破坏试验,对芽胞和白色念珠菌进行抑菌试验。结果　该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近,污染样液除菌后１ ０００ 倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果,２０ 倍稀释可完全破坏ＨＢｓＡｇ 抗原性,和抑制枯草杆菌黑色变种芽胞的生长,１ ６００ 倍稀释可抑制白色念珠菌生长,而原液则不能抑制分离菌株的生长。结论　洋葱假单胞菌可在高浓度（１ ．６％ ）季铵盐消毒剂中大量生长,其对该类消毒剂抗力明显高于大肠杆菌、金黄色葡萄球菌、白色念珠菌、枯草杆菌黑色变种芽胞和ＨＢｓＡｇ,其对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近