Alcoholic and isocaloric diet,but not ovariectomy,influence the apoptosis of bone cells within the alveolar bone crest of rats

ObjectiveStudies suggest that chronic alcoholism as well as oestrogen deficiencies may affect bones in general, including alveolar bone and, by doing so, increase individuals’ susceptibility to develop progressive periodontal disease. This paper aims to verify the influence of chronic alcoholism and/or oestrogen deficiencies in the apoptosis of bone cells of the alveolar bone crest region in rats.DesignInitially, 54 rats were divided into ovariectomized (Ovx) and Sham operated (Sham) groups. Thirty days after surgery, these two groups were equally sub-divided, and received, for 56 days, the following dietary intervention: alcoholic diet (with 20% alcohol solution,), isocaloric diet and ad libitum diet (free diet). Analysis was undertaken by immunohistochemistry, using an antibody to detect apoptosis (anti PARP p-85).ResultsWhen comparing the six experimental groups, no significant differences were observed in the apoptosis of bone cells. Also, there was no significant difference in the quantity of cells undergoing apoptosis when the animals from Ovx groups were compared with those from Sham groups. However, when comparing only different dietary groups, differences were observed between the groups ad libitum and isocaloric, to osteoblasts (p = 0.045); and ad libitum and alcohol, to osteocytes (p = 0.007).ConclusionIt is concluded that ovariectomy was not able to influence the rate of apoptosis of bone cells of the alveolar bone crest region in rats and that a possible influence of diet on apoptosis of osteoblasts and osteocytes cannot be ruled out.     

Emergency management for preventing and controlling nosocomial infection of the 2019 novel coronavirus: implications for the dermatology department

Since cases first emerged in December 2019, COVID-19 (a type of coronavirus) has rapidly become pandemic. This fast-tracked paper (published quickly) from China on COVID-19 is written by dermatologists at the epicentre of the outbreak in Wuhan. Dermatology clinic staff may be at risk because protective equipment is not routinely available, and skin lesions might possibly transmit the virus indirectly. These authors suggest preventive measures based on experience in this and previous coronavirus outbreaks. Online consultation for non-urgent patients reduces the numbers of patients attending clinics. Nurse-led triage, to identify patients with possible COVID-19, at the entrances of hospital and skin clinics directs patients with a cough or fever to a specific COVID-19 area and a dermatologist is consulted if the fever might be related to skin disease. Clinic staff wear N95 masks and observe hand hygiene during consultations. Patients are admitted to a ward only if routine blood tests and chest CT scans exclude COVID-19. Triage will not detect patients who are showing no symptoms but who are developing the disease, so the hospital should provide an on-call expert team to discuss inpatients suspected or diagnosed with COVID-19 and refer them to radiology, respiratory or intensive care colleagues as required. Confirmed cases are managed following local policies. Skin disorders in COVID-19 inpatients can usually be managed remotely using photographs, email and teleconferencing. If necessary a multidisciplinary team (a team of medical staff from different specialties) can meet in the clean area of the isolation ward. If the dermatologist must see the patient, all records should be provided in advance to minimise exposure time. With these precautions, as of 20th February 2020 no infected patients were detected in the Wuhan Dermatology Department. This is a summary of the study: Emergency management for preventing and controlling nosocomial infection of 2019 novel coronavirus: implications for the dermatology department     

反射性共聚焦显微镜对早期蕈样肉芽肿定位诊断与疗效监测的研究

【摘要】 目的 分析早期蕈样肉芽肿（MF）皮损的反射性共聚焦显微镜（RCM）与组织病理学特征的一致性,探讨RCM作为病理学诊断早期MF的辅助价值及动态监测治疗反应的可行性。方法 2014年1月至2018年1月在杭州市第三人民医院皮肤科门诊收集临床疑诊MF病例40例,男26例,女14例,年龄（47.0 ± 17.6）岁。根据总结的早期MF的RCM特征,对活检部位进行初步定位后行组织病理学检查,对比MF的RCM与病理特征。对MF确诊病例进行窄谱中波紫外线联合干扰素治疗,运用RCM对靶向皮损跟踪随访,9个月后评价疗效。结果 在40例临床疑诊MF病例中,根据RCM特征初步诊断典型MF 8例,疑似MF18例,排除14例;根据MF病理学特征诊断典型MF12例,疑似14例,排除14例。一致性分析显示,RCM特征分类与病理诊断结果的Kappa系数为0.848,P < 0.01。RCM与组织病理学特征之间表皮低折光细胞浸润的相关性最高（Kappa系数 = 1,P = 0.005）,其次是红斑期的真皮纤维化（Kappa系数 = 0.714,P = 0.035）。MF的RCM特征随着治疗的进行逐步恢复正常,但直至临床皮损完全缓解,非典型淋巴细胞依然存在。结论 RCM技术可用于早期疑诊MF皮损的病理取材定位,同时可尝试作为一种动态监测MF疗效的方法。     

3D打印模型结合PBL教学法在骨盆骨肿瘤临床教学中的应用

目的:以骨盆骨肿瘤临床教学为例,研究3D打印模型结合以问题为基础的教学方法(PBL)在住院医师规范化培训中的应用价值.方法:选取2019年7-12月在我院参加住院医师规范化培训的住院医师50名,随机分为试验组(3D打印模型结合PBL教学法)和对照组(传统教学法)各25名,比较2组规培生考试成绩及教学调查问卷评分.结果:...     

406例癫痫蝶骨电极结果分析及探讨

目的:探讨蝶骨电极在脑电图中的应用及意义。方法:对406例行蝶骨电极检查的癫痫病人的脑电图进行分析。结果:脑电图单极描记发现正性棘波的病人蝶骨电极阳性率几近100％;而无正性棘波者,蝶骨电极阳性率几乎为零。结论:蝶骨电极的应用明显提高了脑电图对癫痫的诊断率。     

愈合时间对微型种植体支抗稳定性的影响

目的：研究在相同力值牵引下,愈合时间对微型种植体支抗稳定性的影响。方法：在8只Beagle犬上颌根间牙槽骨中植入49颗微型种植体支抗,分别在即刻负载、愈合2周、愈合4周、愈合12周后施以150g水平牵引力,定期注射荧光标志物,负载2个月后处死动物。制作种植体-骨的不脱钙硬组织切片．进行荧光显微镜观察、组织形态学测量和推出试验。采用SAS6．12软件包中的单因素方差分析和SNK法分析各组间差异,相关性分析BIC、BSA值与生物力学性能参数的相关性。结果：4组标本的骨结合百分率（bone—to—implant contaet,BIC）和骨充填率（bone stlrface area,BSA）分别为（46．6±2．72）％、（44．08±3．62）％、（17．07±0．63）％、（32．85±3．01）％及（66．39±2．94）％、（64．96±5．11）％、（36．82±0．08）％、（70．33±4．07）％。愈合4周与其他3组之间均有显著性差异。4组标本种植体-骨界面的最大剪切力分别是（270．4±20．36）N、（270．4±6．97）N、（105.8±3．73）N、（335．07±17．22）N。愈合12周组与其他3组之间均有显著性差异。种植体-骨界面的总吸收能量情况与最大剪切力相似。微型种植体的生物力学性能与BSA的相关系数显著高于其与BIC的相关系数（心0．051。结论：愈合12周是一个较为理想的负载前愈合时间。即刻负载和愈合2周负载也可以接受,但必须注意种植体的即刻固位和即刻稳定性。而愈合4周负载则不利于种植体的稳定。     

FOLFOX化疗对男性患者性激素的影响及补肾益气中药的保护作用

目的 观察FOLFOX联合化疗方案对男性肿瘤术后患者的性激素影响及补肾益气中药的解毒保护作用。 方法 采用随机区组对照和自身对照实验设计,回顾性调查接受FOLFOX联合化疗的男性结直肠癌、胃癌术后患者的黄体生成素（LH）、雌二醇（E2）、泌乳素（PRL）、孕酮(P)、睾酮(T)和促卵泡激素(FSH)的变化。61例分为单纯中药组、FOLFOX组、FOLFOX加中药组和对照组(仅一般支持治疗）4组,治疗均1个月为1个疗程,共6个疗程。分别在治疗前、治疗结束时（治疗6个月时）和治疗12个月时监测各组的LH、E2、PRL、P、T和FSH。 结果 与治疗前及对照组、FOLFOX加中药组和单纯中药组的同期水平比较,FOLFOX组治疗后的LH、PRL和T均明显下降（均P＜0.01）,治疗结束时分别达（4.6±0.4） IU/L、（8.6±0.7）μg/L和（13.1±1.4） IU/L,之后有所回升,但治疗结束后6个月（即治疗开始12个月时）分别为（5.0±0.4） IU/L、（9.9±1.1）μg/L和（14.1±1.5） IU/L,仍低于治疗前水平以及对照组、FOLFOX加中药组和单纯中药组的同期水平（P<0.05或P<0.01）;FOLFOX加中药组治疗后LH亦明显下降（P<0.05）,低达（5.1 ±0.4）IU/L,但治疗结束后6个月时恢复至治疗前和对照组水平,达（6.1±0.5） IU/L;而该组PRL和T下降不明显（均P>0.05）,治疗开始12个月时回升至治疗前和对照组水平。E2和FSH则相反,FOLFOX组E2和FSH治疗后血液浓度明显升高（均P<0.01）,达（135±14）pmol/L和（9.1±1.1） IU/L,治疗开始12个月时分别为（140±15） pmol/L和（9.1±1.0） IU/L,未降至治疗前和对照组水平,亦明显高于FOLFOX加中药组和单纯中药组的同期水平（均P<0.01）;而FOLFOX加中药组治疗后E2和FSH分别为（116±12）pmol/L和（7.1±0.9） IU/L,升高不明显（均P>0.05）。单纯中药组及对照组的上述指标无明显变化（均P>0.05）;各组观察期内P无明显变化（均P>0.05）。 结论 FOLFOX联合化疗方案均可导致男性胃肠癌术后患者多种性激素水平失常,补肾益气类中药有不同程度的解毒保护作用。