β-catenin与wisp-1在结肠癌中的表达及意义

目的：探讨β-catenin与wisp-1 在原发性结肠癌中表达及临床意义。方法：分别用免疫组化Envision法和实时荧光定量PCR法检测β-catenin、wisp-1 蛋白和基因在53例结肠癌及53例配对正常结肠中的表达。分析两因子表达与临床病理的关系。结果：β-catenin、wisp-1蛋白在结肠癌的异常表达率均高于正常结肠中异常表达率（P〈0.05）。结肠癌中β-catenin蛋白异常表达与淋巴结转移、Duke分期和部位有关（P〈0.05）。wisp-1蛋白异常表达与淋巴结转移、Duke分期有关。β-catenin基因在结肠癌中平均表达是其在正常结肠中的3.237倍（P〈0.05），其异常表达与肿瘤部位及淋巴结转移有关（P〈0.05）。wisp-1基因在结肠癌中平均表达是其在正常结肠中的2.908倍（P〈0.05），其异常表达与肿瘤类型有关（P〈0.05）。结论：β-catenin与wisp-1异常表达特点提示两者与结肠癌的发生发展有密切关系。     

Effects of trophic 2,2′, 4,4′-tetrabromodiphenyl ether (BDE-47) exposure in Oreochromis niloticus: A multiple biomarkers analysis

Polybrominated diphenyl esters are emerging environmental contaminants with few toxicological data, being a concern for the scientific community. This study evaluated the effects of BDE-47 on the health of Oreochromis niloticus fish. The animals were exposed to three doses of BDE-47 (0, 0.253, 2.53, 25.3 ng g⁻¹) every 10 days, for 80 days. The BDE-47 affected the hepatosomatic and gonadosomatic index in female and the condition factor by intermediate dose in both sexes. The levels of estradiol decreased and the T4 are increased, but the vitellogenin production was not modulated in male individuals. Changes in AChE, GST, LPO and histopathology were observed while the integrated biomarker response index suggests that the lowest dose of BDE-47 compromised the activity of antioxidant enzymes. The oral exposure to BDE-47 in environmental concentrations is toxic to O. niloticus and the use of multiple biomarkers is an attribution in ecotoxicology studies and biomonitoring programs.     

七氟烷在小鼠中的代谢研究

目的探讨吸入麻醉药七氟烷是否能在小鼠肝及脑组织中进行生物转化,并对其代谢产物六氟异丙醇(HFIP)进行测定。方法在七氟烷麻醉下,取小鼠部分肝及全部脑组织,将其匀浆后于密闭空针中分别加入0.5%、1%及3%七氟烷于37℃恒温箱中孵育6h,分别于0、1、2、4、6h测定气相中HFIP浓度。结果在对小鼠肝脏组织匀浆的孵育中,3种七氟烷孵育浓度均检测出HFIP,且以0.5%七氟烷孵育时HFIP含量最高。在对小鼠脑组织匀浆的孵育中,当七氟烷浓度为0.5%及1%时检测出HFIP,且前者高于后者,但当七氟烷孵育浓度为3%时,未能检出HFIP。结论七氟烷能在小鼠肝及脑组织中代谢分解成HFIP,但随着七氟烷孵化浓度的增加,HFIP的生成却减少。     

结直肠癌肝转移局部治疗的研究进展

结直肠癌是常见的消化道恶性肿瘤，易发生肝转移。外科手术切除肝转移病灶是重要治疗方法之一。随着临床诊疗技术的进步，介入治疗、放射治疗等方法已成为结直肠癌肝转移的重要治疗手段。本文将对结直肠癌肝转移局部治疗的研究进展进行综述。     

Risk prediction of cerebrovascular events with carotid plaque magnetic resonance analysis: A meta-analysis

Background and purpose

It is not conclusive that magnetic resonance (MR)-based carotid atherosclerotic plaque assessment identifies high-risk features associated with cerebrovascular events. We aimed to systematically summarize the association of MR imaging (MRI)-determined intraplaque hemorrhage (IPH), lipid-rich necrotic core (LRNC), and thinning/rupture of the fibrous cap (TRFC) with subsequent ischemic events.

Reduced serologic sensitivity to influenza A virus illness among inactivated influenza vaccinees

We compared ≥4-fold increases in antibody titers by hemagglutination inhibition assay to RT-PCR results among 42 adults with PCR-confirmed influenza A virus illnesses. Serologic sensitivity was higher among unvaccinated (69%, 95% confidence interval [CI] = 48–90%) than vaccinated healthcare personnel (38%, 95% CI = 29–46%) in a 2010–11 prospective cohort.