FK506和RS-61443对大鼠异体肢体移植的联合免疫抑制作用

目的　通过大鼠异体肢体移植模型 ,旨在分析 FK5 0 6和 RS- 6 14 4 3对大鼠异体肢体移植中急性排斥反应的免疫抑制作用。　方法　选择雄性 Wistar和 SD大鼠为供、受体 ,以 FK5 0 6和 RS- 6 14 4 3为免疫抑制剂 ,对照组为术后不用药组 ,实验组根据用药剂量和药物不同分为 6组 ,各组用药时间均为 5周 (每日 1次共 2周 ,然后每周 2次共 3周 ) ,进行了 10 1例异体肢体移植动物实验。观察大鼠一般情况、移植肢体排斥反应及存活时间。　结果　对照组肢体平均存活时间为 (7.0 0± 0 .78)天 ;实验组 1～ 6组移植肢体平均存活时间分别为 (17.0 8± 4 .5 0、2 3.2 0± 5 .0 5、11.19±2 .2 8、16 .33± 1.83、13.33± 3.2 2和 5 8.76± 6 .81)天。　结论　 FK5 0 6和 RS- 6 14 4 3能抑制大鼠同种异体肢体移植术后急性移植排斥反应的发生 ,并能延长移植肢体的存活时间。     

脊髓损伤后中枢性疼痛的实验模型和机制研究进展

中枢性疼痛(central pain,CP)是脊髓损伤(spi-nal cord injury,SCI)的顽固性合并症,为患者主观上感到损伤平面以下皮肤痛觉消失区一种以自发痛为主的疼痛,发生率为11%~94%,严重者为5%~30%。近年来其发生率逐年增高,使很多患者陷于抑郁、药物成瘾、甚至自杀的悲惨境地。许多疼痛     

Effect of N-tosyl-L-phenylalanylchloromethyl Ketone on Tumor Necrosis Factor-alpha -induced NF-κB Activation and Apoptosis in U937 Cell Line

Summary: To investigate the effect of N-tosyl-L-phenylalanylchloromethyl ketone (TPCK) on tumor necrosis factor-alpha-induced NF-κB activation and apoptosis in U937 cell line, changes and subcellular localization of NF-κB/p65 and IκB-α were observed by fluorescencemicroscopy and expression and degradation of IκB-α by flow cytometry. The apoptosis of U937 cells was measured by flow cytometry and electrophoresis of DNA. Immunolfluorescence assay showed that NF-κB/p65,IκB-α only localized in cytoplasm. After TNF-α stimulation, p65 was localized only in nuclei, and IκB-α was only localized in cytoplasm and decreased. The changes of TNF-α stimulation were specifically inhibited by TPCK. Flow cytometry also revealed the downregulation of IκB-α protein during TNF-α-induced apoptosis and the down-regulation was specifically inhibited by TPCK. Flow cytometry also showed the apoptosis of U937 cells after TNF-α induction. DNA ladder can be detected in cells treated by TNF-α. It is concluded that degradation of IκB-α protein and NF-κB/p65 translocation occur during TNF-α-induced apoptosis of U937 cells, suggesting the activation of NF-κB.TPCK-sensitive protease plays an important role in the degradation of IκB-α protein induced by TNF-α in U937 cells. TPCK sensitive protease also plays an important role in the apoptosis of U937 cells induced by TNF-α.     

激光穴位点射疗法对痛风急性期的疗效观察

目的:观察500MW激光风湿治疗机穴位点射治疗痛风急性期的疗效,探讨其作用原理。方法:痛风急性期患者48例,随机分为激光组和对照组,每组24例。激光组用输出波长为810 NM,功率为500 MW激光风湿治疗机,通过穴位点射方法治疗。对照组口服非甾体类消炎药。疗程均为3~7 d,观察患者关节疼痛肿胀情况及不良反应表现。结果:激光组疗效优于对照组,且毒副作用明显小于对照组。结论:激光穴位点射治疗对痛风急性期有良好的治疗作用,且无明显不良反应,患者依从性好。     

18F-氟脱氧葡萄糖PET-CT诊断主动脉壁粥样硬化斑块的价值探讨

目的通过PET-CT明确18F-氟脱氧葡萄糖在主动脉壁粥样硬化斑块中摄取程度,探讨其临床价值。方法60例受检者,包括正常对照者6例,大动脉粥样硬化病例54例。测定主动脉壁粥样硬化部位标准化摄取值（SUV）和CT值,并依据CT值将54例主动脉壁局部18F-FDG高摄取的动脉粥样硬化病例分成3组,软斑块组CT值小于60HU,中等斑块组CT值介于60￣100HU之间,钙化斑块组CT值大于100HU。第4组为正常对照组。结果软斑块组42个测量部位的平均SUV为1.553±0.486;中等斑块组30个测量部位的平均SUV为1.393±0.296;钙化斑块组36个测量部位的平均SUV为1.354±0.189;正常对照组33个测量部位的平均SUV为1.102±0.141。多组之间比较差异具有统计学意义（F=678.909,P=0.000）;正常组与各斑块组比较差异具有统计学意义。结论18F-FDG在主动脉粥样硬化斑块中具有不同程度的摄取,其中软斑块对18F-FDG的摄取最高,提示18F-FDG PET-CT是诊断不稳定性主动脉粥样硬化斑块的有价值方法。     

子宫内膜异位症患者子宫内膜人白细胞抗原的免疫组化研究

目的 研究子宫内膜异位症(内异症)患者在位和异位子宫内膜人白细胞抗原HLA—DR的表达。方法 采用酶标链霉亲合素—生物素(LSAB)技术对19例内异症不孕患者在位和异位子宫内膜腺细胞HLA—DR抗原的表达进行免疫组化研究，以19例非子宫内膜异位症的不孕患者作为对照。结果 内异症患者在位和异位子宫内膜腺细胞HLA—DR抗原的表达较对照组明显增强(P＜0．01)。结论 子宫内膜HLA—DR抗原的异常表达可能参与了内异症免疫学异常的发生。     

Point Mutation in the Parkin Gene on Patients with Parkinson''''s Disease

Summary;To investigate the distribution of possible novel mutations from parkin gene in variant sub-set of patients with Parkinson’s disease(PD)in China and explore whether parkin gene plays an im-portant role in the pathogenesis of PD,70 patients were divided into early-onset group and late-onsetgroup; 70 healthy subjects were included as controls.Genomic DNA from 70 normal controls andfrom those of PD patients were extracted from peripheral blood leukocytes by using standard proce-dures.Mutations of parkin gene(exon 1—12)in all the subjects were screened by PCR-single strandconformation polymorphism(SSCP),and further sequencing was performed in tue samples with ab-normal SSCP results,in order to confirm the mutation and its location.A new missense mutationGly284Arg in a patient and 3 abnormal bands in SSCP electrophoresis from samples of another 3 pa-tients were found.All the DNA variants were sourced from the samples of the patients with early-on-set PD.It was concluded that Parkin point mutation a     

类风湿因子IgM-RF、IgG-RF和IgA-RF的临床意义分析

目的　探讨 3种类风湿因子 (RF)诊断类风湿关节炎 (RA)、反映病情活动度和疗效的临床意义。方法对关节痛 /关节炎 (A组 )、确诊的RA (B组 )、其它免疫结缔组织病 (C组 )患者和正常人 (D组 )共 6 18例用酶联免疫法进行 3种RF的动态检测并随访观察。结果　 4组人群中 3种RF的阳性率和浓度以B组最高 [IgM RF为 92 4 % ,(182 5± 5 1 4 )IU/ml;IgG RF为 6 7 8% ,(2 2 9 7± 75 6 )IU/ml;IgA RF为 78 8% ,(2 35 8± 80 7)IU/ml],组间比较依次为B组 >A组 >C和D组 (均为P <0 0 5 )。B组中高活动期RA患者的 3种RF浓度明显高于其它 3组 (P <0 0 5 ) ,IgM RF和IgA RF在稳定期和低活动期较高活动期均明显降低 (均为P <0 0 5 ) ,而IgG RF变化无显著性。随访 3～ 18个月 ,A组中最后确诊为RA的比率以 3种RF同时阳性者最高 (10 0 0 % ) ,2种RF同时阳性者次之(90 9%～ 93 3% ) ,以单一RF作比较则IgG RF >IgA RF >IgM RF阳性者。结论　早期诊断RA以IgM RF的敏感性最高 ,以IgG RF的特异性最高 ,任何 2种以上RF阳性可提高RA诊断的可靠性 ,RF浓度可作为判断RA活动度及药物治疗效果的指标之一。     

具有骨诱导活性的仿生骨基质材料的制备

目的将一种具有与骨形态发生蛋白2相似骨诱导活性的多肽p24共价结合于改性聚（丙交酯-co-乙交酯）基质材料上，以制备出具有骨诱导活性的仿生骨基质材料。方法将活性多肽p24通过交联剂共价结合于改性PLGA材料上作为实验组；以未加交联剂多肽溶液和材料简单混合反应为对照组，通过X射线光电子光谱法和扫描电镜检测交联情况；同时对两组材料进行钙离子吸附实验，初步评价其仿生矿化能力。结果XPS检测结果表明，实验组及对照组材料表面均已结合硫元素，两者含有硫元素含量分别为1.50％及0.09％；钙离子吸附实验结果表明，12h及24h时实验组材料的钙离子吸附量分别为0，126mg及0．231mg；12h及24h时对照组材料的钙离子吸附量分别为0．053、0．102mg。多肽交联之材料较混合之材料的钙离子吸附能力显著增强。结论在改性PLGA三嵌段材料上固定了BMP2活性多肽，为以后发挥其骨诱导活性修复骨缺损奠定了良好基础。