应用GQI技术对小脑髓质的初步研究

目的　应用基于GQI的弥散成像方法,对小脑的纤维束进行重建和显示,为小脑疾病的影像诊断进行解剖学和技术上的探索。  方法　首先把10例脑连接组项目的磁共振弥散数据导入DSI-studio软件,然后依次用该软件中的GQI（Generalized Q-sampling imaging）成像技术和流线算法（streamline algorithm）对与小脑有关的纤维束进行重建和显示。  结果　（1）在DSI-studio软件的二维和三维界面中显示了小脑三对脚的位置和交叉情况;（2）显示了小脑3对脚与脊髓、延髓、脑桥、中脑、端脑等的连接情况及其主要纤维成分。  结论　应用GQI技术可以对小脑白质进行重建和显示,为小脑疾病的影像学诊断提供技术和结构学上的帮助。     

团体心理辅导改善小学生孤独状况

目的探讨团体心理辅导对小学生孤独状况的影响。方法使用儿童孤独量表问卷(CLS)对宁波市某小学三年级两个班的学生进行问卷调查,从两个班选出量表总分前30名的学生作为被试团体,将这30名学生随机分成两组,一组为实验组,另一组为对照组,对实验组给予8次团体心理辅导,并在辅导后再次使用儿童孤独量表问卷对实验组和对照组进行问卷调查。结果①当前小学生孤独状况比较严重。②实验组在干预前后的孤独状况有显著差异(t=5.209,P0.01)。③干预后,两组小学生孤独状况存在显著差异(t=-2.853,P0.05)。结论团体辅导对小学生孤独状况有一定的改善作用。     

父母养育方式与大学生性别角色特质期待的相关性

目的探究父母养育方式与大学生性别角色特质期待的关系。方法采用父母养育方式量表(EMBU)和自编性别角色特质期待问卷,对192名大学生进行研究。结果 1男女大学生对自我性别角色特质期待除无私、真诚、宽容、勤俭外,其余特质差异显著(P0.05或P0.001);异性性别角色期待中除善良、细心、体贴、无私、真诚、现实、宽容、健谈外,其余特质差异显著(P0.001);2男女大学生在父亲温暖理解(t=-2.077,P0.05)、母亲温暖理解(t=-2.567,P0.05)、父亲过度保护(t=2.216,P0.05)3个因子上差异显著,城镇被试和农村被试在父亲偏爱被试(t=-2.280,P0.05)和母亲偏爱被试(t=-2.077,P0.05)两个因子上差异显著;3自我性别角色特质期待中,母亲拒绝否认与细心、体贴、善良、上进、真诚等特质呈显著负相关;父亲偏爱被试与细心、温柔特质呈显著负相关;4异性性别角色特质期待中,父母拒绝否认与善良、上进、真诚、稳重、温柔、宽容、体贴、理性等特质呈显著负相关,父母温暖理解与勇敢、稳重等特质期待呈显著正相关。结论父母养育方式对大学生性别角色特质期待有影响。     

Expression analysis of BRUCE protein in esophageal squamous cell carcinoma

Apoptosis is a form of cell death in response to diverse stressful physiological or pathological stimuli. One of the most important gene families involved in apoptosis is inhibitors of apoptosis. As a member of inhibitors of apoptosis, BRUCE can suppress apoptosis and promote cell division. Because esophageal squamous cell carcinoma (ESCC) cells, as well as other cancer cells, are immortal, our aim in this study was to analyze BRUCE protein expression in ESCC and evaluate its correlation with tumoral clinicopathologic features. Fifty ESCC specimens were examined for BRUCE protein expression using immunohistochemistry. A defined scoring method was applied. BRUCE protein was detected in 82% of tumors. Tumor progression stage and invasion depth correlated significantly with BRUCE protein expression (P = .019 and .005, respectively). Furthermore, association of BRUCE expression with tumor location was near significant (P = .058). The correlation of BRUCE overexpression in ESCC and disease aggressiveness may confirm the importance of BRUCE in ESCC progression and invasiveness. Therefore, BRUCE protein may be a molecular marker for aggressive ESCC and, thus, a potential therapeutic target to inhibit tumor cell progression and invasion.     

Monte Carlo simulation of activity measurement of 123I, 111In and 153Sm with a radionuclide calibrator

The response of radionuclide calibrators for radionuclides with high abundances of high energy X-rays is very sensitive to changes in the source geometry. The magnitude of this effect was explored for Bqmetr (Konsorcium BQM, CR) calibrators with ¹²³I, ¹¹¹In and ¹⁵³Sm in several vial and syringe geometries. Dependencies of chamber responses on solution volume and container position were calculated using MCNP transport code. The possibility of usage of an additional copper filter to reduce chamber sensitivity was studied and found to be suitable.     

Retrospective analysis of therapeutic effect and prognostic factors on early glottic carcinoma

ObjectiveTo investigate the therapeutic effect of surgery, radiotherapy and photodynamic therapy on early glottic carcinoma and prognostic factors.MethodsRetrospective analysis of 202 cases with early glottic carcinoma (Tis-T2N0M0) underwent surgery (n = 152), radiotherapy (n = 20) and PDT (n = 30) from 2000 to 2013 The KPS score, the disease-free survival (DFS), overall survival (OS), local control (LC), larynx preservation rate, laryngeal function were evaluated. The methods of χ ²test or Fisher's exact probability method, Kaplan Meier method, log-rank test of Kaplan-Meier method, Cox proportional hazards model were used to analyze the data.ResultsThere was no statistical significance in OS, DFS and LC among the three groups. The laryngeal function preservation rate of RT group, PDT group and Surgery group were 90%, 86.7% and 65.1% respectively, with the former two groups significantly superior to Surgery group. While there is no statistical significance between RT group and PDT group. Single factor analysis showed that KPS score before treatment, vocal fold mobility limitation and differentiation degree could have an effect on prognosis. Multivariate regression analyses indicated that anterior commissure invasion, T stage and KPS score before treatment were independent adverse prognostic factors for OS. T stage and differentiation degree were adverse prognostic factors for DFS. T stage was also an adverse factor of LC. Thirty-three cases experienced local recurrence or cervical lymph node metastasis. Three groups showed no statistical difference in local recurrence or lymph node metastasis, with twenty-two cases in Surgery group, four in RT group and seven in PDT group.ConclusionsThe therapeutic effect was approximate in surgery group, radiotherapy group and photodynamic group, and all three treatment regimens achieved good clinical effect. Radiotherapy and photodynamic therapy may be the first or very important treatment on early stage glottic squamous cell cancer (Tis ∼ T₂N₀M₀). However only 6 patients underwent PDT for T2 disease, making definitive treatment conclusions for this subgroup unclear.     

宁波市城区学生体质特征分析

【目的】了解宁波市城区学生的体质状况，为有关决策部门提供宁波市城区学生体质状况方面的相关资料，为改善宁波市城区学生体质状况提供可靠的参考依据，为更好地开展全民健身活动服务。【方法】采用体质监测、文献资料调研和数理统计等方法。【结果】宁波市城区学生的身体形态、机能、素质等指标，随着年龄的增长而呈上升趋势，增幅随着年龄的增长而呈下降趋势。其身体形态各指标在男生16岁前、女生15岁前变化较大，之后变化缓慢，逐渐过渡到相对稳定阶段，大部分指标与全国同龄人基本相近，少部分指标与全国同龄人存在着差异。男生17岁前、女生14岁前身体机能指标，变化较大，之后变化缓慢，逐渐过渡到相对稳定阶段，各指标与全国同龄人存在着明显的差异。身体素质指标在男生16岁前、女生13岁前变化较大，之后变化缓慢，逐渐过渡到相对稳定阶段；少部分指标与全国同龄人基本相近，大部分指标与全国同龄人存在着差异。【结论】宁波市城区学生的身体形态、机能、素质等各项指标符合人体一般生长发育规律，其中大部分指标与全国同龄人存在差异，并好于全国同龄人。     

结构方程模型在生育保险病种费用影响因素分析中的应用

目的:研究生育保险病种费用影响因素,为生育保险按病种付费提供依据.方法:抽取南京市4家医院2002～2004年生育保险参保人员病历首页以及财务结算帐单1 525份,用结构方程模型(SEM)方法分析.结果:临床特征、医护质量和社会经济特征三个隐变量对住院费用的影响系数分别为0.9334、0.3194、0.2230,病种临床特征对住院费用影响最大.结论:SEM分析生育保险病种住院费用影响因素收到很好的效果.     

精神分裂症患者染色体D6S296等微卫星标志的多态性分析

目的　探讨人类第 6号染色体上与精神分裂症有关的易感基因位点。方法　研究对象为上海地区 1 78例精神分裂症患者 (其中 82例为病程在 1 0年以上的慢性精神分裂症患者 ) ,以 88名健康人为正常对照。应用扩增片段长度多态性技术 (AFLP) ,观察D6S470、D6S2 74、D6S2 96和D9S1 75等 4个微卫星标志多态性的分布。结果　精神分裂症患者和健康人D6S470、D6S2 74、D6S2 96和D9S1 75微卫星标志上各等位基因频率的分布均符合Hardy Weinberg平衡。慢性精神分裂症组患者D6S2 96的 2 64bp等位基因频率为 0 .1 688,而健康人组为 0 .0 390 ,差异有非常显著意义 (χ2 =1 7.68,P<0 .0 0 1 ) ;其他微卫星标志的各等位基因频率分布在各组之间的差异无显著意义。D6S2 96的 2 64bp等位基因与慢性精神分裂症间存在强烈关联 (RR =8.30 ,χ2 =1 3 .91 ,υ=1 ,P <0 .0 0 1 )。结论　人类 6号染色体上D6S2 96微卫星标志区域可能存在精神分裂症的易感基因