临床药师对1例急性重症胰腺炎患者的药学服务

临床药师对1例急性重症胰腺炎患者进行药学监护,与医师共同制订治疗方案：在抑酸、抗菌、抑制胰腺外分泌药物和胰酶制剂、营养支持和中药的合理使用方面提供用药建议。确保了患者用药安全有效。     

动脉粥样硬化危险因素的研究进展

已经确定的动脉粥样硬化的传统危险因素,如高脂血症、高血压、抽烟、糖尿病等并不能解释和预测所有心脑血管临床事件的发生。随着流行病学调查和实验室工作的深入,发现一些新的危险因素例如循环白细胞数量、血浆纤维蛋白原、尿酸、脂肪组织分布等在动脉粥样硬化的形成与发展过程中越来越发挥着举足轻重的作用。鉴于此,本研究针对相关最新研究进行简要阐述,力图为动脉粥样硬化的恶性演进提供相关实验与理论依据。     

护理风险管理在援藏期间护理工作中的应用

目的探讨2013年援藏期间,运用护理风险管理对受援单位护理工作进行管理的效果。方法以实施护理风险管理前后的护理工作进行对比,通过护理风险的识别及采取对策,保证管理的效果。结果当地护士的护理风险意识增强,护理质量有明显提升（P＜0．05）。结论护理风险管理是值得推广的,但在西藏地区开展护理工作应因地制宜,考虑多方面的因素,促进民族团结。     

浅议医疗设备招标采购监督的难点与对策

针对医疗设备院内招标采购中存在的监督难点及原因进行分析,提出从制度、程序、操作等方面完善监督机制,建议通过加强标书审核、公开采购信息、严审合同条款、强化验收环节、后续监管等方面强化过程监督。医院招标采购活动既要做到严格程序、规范操作,又要注意效率和追求实效。     

Effects of OX40–OX40 ligand interaction on the levels of ROS and Cyclophilin A in C57BL/6J mice atherogenesis

Background

An increasing amount of evidence shows that the OX40–OX40L interaction serves an important function in atherosclerosis. However, the mechanism of the OX40 signaling pathway remains unclear. This study investigates the effect of OX40–OX40L interaction on the levels of intracellular reactive oxygen species (ROS) and the secretion of Cyclophilin A (CyPA) in C57BL/6J mice atherogenesis.

Methods

The atherosclerotic plaque model was established by placing a rapid perivascular carotid collar on C57BL/6J mice fed with a western-type diet. In vivo, the expressions of CyPA in mouse plaque and lymphocytes were detected by immunohistochemical and Western blot analyses, respectively. In vitro, the expression of CyPA protein in cultured lymphocytes of C57BL/6J mice was assessed by using Western blot analysis. The level of ROS was detected through flow cytometry.

Results

CyPA expression was significantly increased in the atherosclerotic lesions and lymphocytes from C57BL/6J mice. The ROS levels in OX40⁺-lymphocytes were increased in vitro and in vivo. After stimulating the OX40–OX40L interaction, the ROS and CyPA levels in lymphocytes were obviously increased in vitro, whereas anti-OX40L mAb significantly down-regulated the anti-OX40 mAb-induced ROS generation and inhibited CyPA secretion in lymphocytes.

Conclusion

The OX40–OX40L interaction up-regulates intracellular levels of ROS in C57BL/6J mice and increases CyPA secretion in lymphocytes. Increased CyPA secretion may serve an important function in atherosclerotic plaque formation.     

miR-449a Regulates Proliferation and Chemosensitivity to Cisplatin by Targeting Cyclin D1 and BCL2 in SGC7901 Cells

Background

Recently, several miRNAs have been determined as tumor suppressors in various cancers, such as microRNA-449a. However, the exact molecular mechanisms underlying miR-449a regulated cell proliferation and chemosensitivity in gastric cancer cells have not been well documented.

Aim

The present study was designed to test whether miR-449a mediates cell proliferation and chemosensitivity in gastric cancer cells via regulating cyclin D1 and BCL2.

Methods

In vitro, the ability of cell proliferation and cell viability were measured by MTT assay; cell cycle and cell apoptosis was detected by FCM. qRT-PCR was used to measure the expression of miR-449a. Western blot and real-time PCR assays were used to detect the expression of cyclin D1 and BCL2 in gastric cancer cell line SGC7901.

Results

miR-449a expression was downregulated in gastric cancer cell line SGC7901 and human gastric cancer tissues, compared to the gastric epithelial cell line GES-1 and matched non-tumor associated tissues. Upregulation of miR-449a reduced the proliferation of SGC7901 cells. Ectopic expression of miR-449a decreased the percentage of S phase cells, increased the percentage of G1/G0 phase cells and increased the apoptosis induced by cisplatin. Moreover, miR-449a inhibited SGC7901 cells proliferation and enhanced cisplatin chemosensitivity by downregulating expression of BCL2 and cyclin D1, respectively, via directly targeting the 3′-untranslated regions of BCL2 and cyclin D1 mRNA.

Conclusions

This is the first report to provide evidence that miR-449a could modulate cell cycle and apoptosis through regulating cyclin D1 and BCL2 expression in SGC7901 cells.     

军队离休老干部的心理问题及护理对策

目的探讨军队离休老干部患者的常见心理问题,制定并实施相应的护理措施。方法收集2011年1月至2013年1月期间,我科住院治疗的离休老干部200例,调查分析其心理特点,制定并实施合理有效的护理方案。结果患者的护理满意度达96.0%,护理后症状自评量表（SCL-90）各项因子评分均显著降低。结论离休老干部患者多存在不同程度的心理问题,护理人员客观准确地评估患者的心理状况,合理实施有效的心理护理措施,能够确保其身心健康,改善其生活质量。     

Clinicopathological significance of PD-L1 expression in colorectal cancer: Impact of PD-L1 expression on pFOXO1 expression

ObjectiveThis study aimed to evaluate the clinicopathological significance of PD-L1 expression and its impact on phospho-Forkhead box O 1 (pFOXO1) expression in colorectal cancer (CRC).MethodsImmunohistochemical analysis for PD-L1 and pFOXO1 was performed on 265 human CRC tissues. PD-L1 expression was evaluated in the tumor and immune cells. The impact of PD-L1 expression on survival was investigated in relation to the pattern of pFOXO1 expression.ResultsPD-L1 was expressed in 25 (9.4%) and 41 (17.7%) patients in the tumor and immune cells of the 265 CRC tissues, respectively. PD-L1 expression in immune cells (I-PD-L1) was significantly correlated with less lymphatic invasion, lymph node metastasis, and distant metastasis and lower pT and pTNM stages. Additionally, there was a significant correlation between PD-L1 expression in tumor cells (T-PD-L1) and tumor location (right colon), but not the other clinicopathological characteristics. pFOXO1 expression was significantly lower in CRC with high I-PD-L1 expression than in CRC with low or negative I-PD-L1 expression. However, there was no significant correlation between pFOXO1 and T-PD-L1 expression in CRC. Patients with positive pFOXO1 and low or negative I-PD-L1 expression exhibited the worst survival among patients with CRC.ConclusionCollectively, our results indicate that I-PD-L1 expression was significantly correlated with favorable tumor behaviors and better survival. In addition, patients with high I-PD-L1 and low pFOXO1 expressions had a favorable prognosis than those with other I-PD-L1 and pFOXO1 expression patterns.     

Clinical and radiological outcomes after treatment of sagittal fracture of mandibular condyle (SFMC) by using occlusal splint in children

This study was designed to investigate the effects of occlusal splints in the treatment of sagittal fractures of the mandibular condyle in children. From January 1995 to December 2011, 37 sagittal fractures of the mandibular condyle in 30 patients aged 4–8 years old were included in this study. All the patients were treated with 1–2 mm occlusal splints in the molar region. The mouths of the patients were kept slightly open by the occlusal splints for 3–6 months, and we reviewed the clinical and radiological remodelling of the affected condyles after treatment. Excellent (n = 20) and good (n = 10) clinical outcomes were achieved with full radiological remodelling seen in 19 and partial remodelling in 11. Treatment with occlusal splints is effective in delivering good results and function with minimal morbidity in children with sagittal fractures of the condyle, while permitting ongoing remodelling and growth in the short term.