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Kono Y Yusnita Y Mohd Ali AR Maizan M Sharifah SH Fauzia O Kubo M Aziz AJ 《Archives of virology》2002,147(8):1623-1630
Summary. A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection.
The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR
was performed. A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. The sequence data of the product revealed that the partial gene of Oya virus
S-RNA segment had 65–70% homology with published cDNA sequences of Simbu serogroup viruses. The phylogenetic analysis of the
data showed that the Oya virus is grouped in Simbu serogroup, but is genetically distinct from the serogroup viruses that
have been analyzed molecularly. Serological surveys revealed that the virus distributed widely and densely in Malaysia.
Received January 5, 2002; accepted April 16, 2002 Published online July 19, 2002 相似文献
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A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutralizing antibody to Nipah virus, and application of samples from different animal species. Forty of 200 swine reference sera examined were positive by the ELISA, of which thirty seven were found positive by serum neutralization test. Sera from a total of 131 fruit bats captured in Malaysia were also tested and all found negative by the both tests. It is considered that the solid-phase blocking ELISA can be used as a screening test for Nipah virus infection followed by the serum neutralization test as confirmatory test. 相似文献
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Min CS Bhatia S Kamaruddin AH 《Artificial cells, blood substitutes, and immobilization biotechnology》1999,27(5-6):417-421
Continuous hydrolysis of palm oil triglyceride in organic solvent using immobilized Candida rugosa on the Amberlite MB-1 as a source of immobilized lipase was studied in packed bed reactor. The enzymatic kinetics of hydrolysis reaction was studied by changing the substrate concentration, reaction temperature and residence time(tau) in the reactor. At 55 degrees C, the optimum water concentration was found to be 15 % weight per volume of solution (%w/v). The Michaelis-Menten kinetic model was used to obtain the reaction parameters, Km(app) and V max(app). The activation energies were found to be quite low indicating that the lipase-catalyzed process is controlled by diffusion of substrates. The Michaelis-Menten kinetic model was found to be suitable at low water concentration 10-15 %w/v of solution. At higher water concentration, substrate inhibition model was used for data analysis. Reactor operation was found to play an important role in the palm oil hydrolysis kinetic. 相似文献
5.
The aim of this study, is to determine whether the fine characteristics of the fetal heart sounds could be used to identify intrauterine growth retarded fetuses. A preliminary evaluation, was conducted to compare these characteristics between intrauterine growth retarded fetuses and normal fetuses in the antenatal period after 36 weeks of gestation. Altogether, 7 IUGR fetuses were compared with 12 normal fetuses. An instrument named the Fetal Frequency Phonocardiogram was designed for this purpose. When connected to a personal computer and with a software programme specially written, the fetal heart sound characteristics were analysed. After detailed analysis, there were 3 significant differences between IUGR and normal fetuses, all of which gave a p-value of < 0.01. The frequency of the first heart sound was significantly higher in the IUGR fetuses compared to normal fetuses. The ratio of the amplitude of the first heart sound over the second heart sound was higher in the IUGR group. Finally, the ratio of the time between the first and second heart sound over the cardiac cycle was shorter in the IUGR fetuses. Fetal heart sound analysis, may provide a simple non-invasive method of detecting and monitoring fetuses at risk in the antenatal period. 相似文献
6.
Ashish Soni Pankaj Kumar Das Abdul Wahab Hashmi Mohammad Yusuf Hesam Kamyab Shreeshivadasan Chelliapan 《Sustainable Chemistry and Pharmacy》2022
Strict environmental concern, depletion, and price hike of building construction materials are driving scientific studies for the search of alternative materials for building construction. To this end sustainable building materials could be a fruitful solution. This review aims to discern the environmental efficacy of solid waste management (SWM) and its relationship with four stimuli i.e., economic structure, regulatory structure, science, and time. The study also highlights the investigation of governance network to figure out the regulatory structure and governance of waste management. Extensive details on solid waste with their sources, recycling potential and their current utilization for substantial development are outlined. A throughout of the production process, properties, advantages, disadvantages, and the global economy of building material developed through recycling of solid waste are discussed. This article also deals with the sustainability, social, and environmental impact of green building materials. The study identifies the future direction for the effective utilization of solid waste for developing building materials. Further, the scope of the present also focusses on the concept of circular economy for developing construction materials through recycling of solid waste, which provides an easy reference for solid waste processing towards sustainability. 相似文献
7.
Bovine theileriosis is a serious threat to livestock worldwide. Uncertainty around species prevalence, antigenic diversity and genotypes of strains make it difficult to assess the impact of this parasite and to provide necessary treatment. We aimed to characterize genotypic diversity, phylogeny and prevalence of Theileria orientalis parasites from the states of Telangana and Andhra Pradesh, India by collecting bovine blood samples from the major districts of the two states. Bioinformatic analysis identified antigenic diversity among the prevalent parasite strains using major piroplasm surface protein (MPSP) gene. Our study revealed a prevalence rate of 4.8% (n = 41/862) of T. orientalis parasites in bovine animals and a new genotype of T. orientalis parasite which was not previously reported in India. The emergence of these new genotypes could be an explanation for the frequent outbreaks of bovine theileriosis. Further, whole genome sequencing of T. orientalis strains will help to elucidate the genetic factors relevant for transmissibility and virulence as well as vaccine and new drug development. 相似文献
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9.
Rome foundation Asian working team report: Real world treatment experience of Asian patients with functional bowel disorders 下载免费PDF全文
Lishou Xiong Xiaorong Gong Kewin Tien‐Ho Siah Nitesh Pratap Uday Chand Ghoshal Murdani Abdullah Ari Fahrial Syam Young‐Tae Bak Myung‐gyu Choi Ching‐Liang Lu Sutep Gonlachanvit Andrew Seng Boon Chua Kuck‐Meng Chong Jane D Ricaforte‐Campos Quan Shi Xiaohua Hou William E Whitehead Kok‐Ann Gwee Minhu Chen 《Journal of gastroenterology and hepatology》2017,32(8):1450-1456
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