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目的探讨多重耐药的导管相关性血流感染(catheter-related blood stream infection,CRBSI)发病情况,为制定和采取预防控制措施提供依据。方法对2011年1月—2012年2月住院患者中多重耐药的导管相关性血流感染病例进行统计分析。结果导管相关性血流感染发生率4.1%,其多重耐药感染发生率3.0%,主要为革兰阴性菌为91.4%,凝固酶阴性葡萄球菌、肺炎克雷伯菌、肠球菌、鲍曼不动杆菌、大肠埃希菌、耐甲氧西林金黄色葡萄球菌分别在多重耐药中占33.2%、13.6%、10.5%、10.1%、9.3%、6.4%。结论多重耐药菌对常用抗菌药物几乎全部耐药,重视病原菌监测,对控制多重耐药的导管相关性血流感染及临床合理应用抗菌药物有指导意义。 相似文献
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Jun-Yan Li Ruilin Jing Hongyi Wei Minghao Wang Qi Xiaowei Haoxi Liu Liu Jian Jiang-Hua Ou Wei-Hua Jiang Fu-Guo Tian Yuan Sheng Heng-Yu Li Hong Xu Rui-Shan Zhang Ai-Hua Guan Ke Liu Hong-Chuan Jiang Yu Ren Jian-Jun He Weiwei Huang Ning Liao Xiangjun Cai Jia Ming Rui Ling Yan Xu Chun-Yan Hu Jianguo Zhang Baoliang Guo Lizhi Ouyang Ping Shuai Zhenzhen Liu Ling Zhong Zhen Zeng Ting Zhang Zhaoling Xuan Xuanni Tan Junbin Liang Qinwen Pan Li Chen Fan Zhang Lin-Jun Fan Yi Zhang Xinhua Yang Jing BoLi Chongjian Chen Jun Jiang 《International journal of cancer. Journal international du cancer》2019,144(2):281-289
Multigene panel testing of breast cancer predisposition genes have been extensively conducted in Europe and America, which is relatively rare in Asia however. In this study, we assessed the frequency of germline mutations in 40 cancer predisposition genes, including BRCA1 and BRCA2, among a large cohort of Chinese patients with high hereditary risk of BC. From 2015 to 2016, consecutive BC patients from 26 centers of China with high hereditary risk were recruited (n = 937). Clinical information was collected and next-generation sequencing (NGS) was performed using blood samples of participants to identify germline mutations. In total, we acquired 223 patients with putative germline mutations, including 159 in BRCA1/2, 61 in 15 other BC susceptibility genes and 3 in both BRCA1/2 and non-BRCA1/2 gene. Major mutant non-BRCA1/2 genes were TP53 (n = 18), PALB2 (n = 11), CHEK2 (n = 6), ATM (n = 6) and BARD1 (n = 5). No factors predicted pathologic mutations in non-BRCA1/2 genes when treated as a whole. TP53 mutations were associated with HER-2 positive BC and younger age at diagnosis; and CHEK2 and PALB2 mutations were enriched in patients with luminal BC. Among high hereditary risk Chinese BC patients, 23.8% contained germline mutations, including 6.8% in non-BRCA1/2 genes. TP53 and PALB2 had a relatively high mutation rate (1.9 and 1.2%). Although no factors predicted for detrimental mutations in non-BRCA1/2 genes, some clinical features were associated with mutations of several particular genes. 相似文献
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Background
Tumorigenesis is thought to be the consequence of gene mutation and disordered gene expression. However, the detailed molecular mechanism underlying the development and progress of colon cancer have not been elucidate completely. This study aimed to find out the genes associated with cancer biological pathways involved in transformation and tumorigenesis.Methods
Normal intestinal cell line 6 (IEC-6) cells were transformed to cancer cells by treatment with cancerogenic agent of N-methyl-N''-nitro-N-nitrosoguanidine (MNNG) and Phorbol 12-myristate 13 acetate (PMA). Then we investigated the altered gene expression of transformed IEC-6 cells by the microarray containing 113 genes associated with cancer pathway. Also the altered miRNAs of transformed IEC-6 cells were analyzed by array hybridization (miRCURY Array v9.2, Exiqon). The levels of acetylated histone H3 in transformed IEC-6 cells was evaluated by western blot.Results
Cell proliferation was significantly increased as IEC-6 cells were transformed and tumor xenografts could be detected in animals as transformed IEC-6 cells were inoculated subcutaneously in nude mice. Result of microarray showed nine genes were increased and two decreased, as well as 13 miRNA were increased and 97 decreased. Verification by real-time PCR implies that the data obtained from microarray analysis were reliable. Western blot showed the levels of acetylated histone H3 were increased dramatically after MNNG/PMA treatment.Conclusion
Our results showed many important biological pathways and miRNAs were involved in transformation and tumorigenesis of IEC-6 cells, which suggested the transformation of normal cells was involved with large mount of genetic and epigenetic variation. 相似文献5.
目的 通过调查中国部署前维和军医战伤救治能力现状,分析维和军医战伤救治技能培训中存在的问题,为完善培训内容提供借鉴.方法 采取整群抽样法,纳入部署前中国维和分队一级医院军医作为研究对象,通过问卷调查法、理论考核、桌面推演和实训演练(包括现场评估、检伤分类、胸腔闭式引流术),对35名完成战伤救治培训的维和军医进行调查.问... 相似文献
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ABC分类法在医疗用品库存管理中的应用 总被引:3,自引:0,他引:3
结合医院库存用品的特点和特殊的经营性质,在阐述重点库存控制-ABC分类法的思想和操作步骤的基础上,提出了该方法在医院实际应用中应该注意的问题. 相似文献
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目的探讨常规加压法和桡动脉止血器法在经桡动脉径路介入术后止血中的安全性及有效性。方法选取Allen试验阳性并经桡动脉径路介入的413例患者,按止血方法分为3组:常规组(87例),TRBand止血器组(122例).Adapty止血器组(204例),观察其止血效果、止血耗时、压迫侧大拇指血氧饱和度、手部肿胀情况、局部组织坏死等。结果常规组止血效果为100%,TR-Band组为90.1%,Adapty组为97.5%,各组间有统计学差异(P〈0.05),常规组初始成功压迫止血的时间为14.5±5.7min、完全止血时间为9.7±4.3h,均长于TRBand组的7.5±3.8min和6.9±4.2h及Adapty组的5.9±3.1min和6.3±3.7h(P〈0.05),常规组手部肿胀率明显高于TRBand组和Adapty组(P〈0.05),但无局部皮肤坏死发生,而TRBand组和Adapty组均有发生,与术后活动少和观察不及时有关,3种止血方法对末梢动脉循环均未见不良影响(P〉0.05)。结论采用TRBand和Adapty桡动脉止血器对桡动脉止血安全、有效,能很好提高介入效率。 相似文献
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目的:在ActiveOne生理信号采集系统基础上采用Labview和VC编程实现基于视觉诱发电位的脑机接口实时系统。方法:数据采集软件采用Labview编程实现,采用VC编程实现脑机接口人机界面、实时信号处理及动态链接库,Labview和VC通过动态链接库共享内存实现数据传输。视觉刺激界面设计采用了多媒体定时、DirectDraw技术和并口输出技术。采用累加平均与5点平均滤波提取视觉诱发电位信号,再通过计算相关系数实现信号识别。结果:实验表明,刺激模块能产生有效的视觉刺激。基于动态链接库的数据传输能满足系统要求,实现VC与Labview程序的同步控制。结论:本文提出的实时信号处理方法能提高信噪比,实现视觉诱发电位的提取与识别,判断出受试者所注视的目标,并将结果实时反馈到人机界面.实现了脑机接口实时系统。 相似文献
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目的用视盘损害分级(disc damage likelihood scale,DDLS)方法评估原发性慢性闭角型青光眼(primarychronic angle-closure glaucoma,PCACG)视神经损害,探讨其与光学断层相干扫描仪(optical coherence tomography,OCT)检测视神经纤维厚度参数的相关性。方法收集2009年10月至2010年8月我院眼科PCACG患者共38例(73眼),通过双面Volk 90D前置镜在裂隙灯下对视盘直径及其盘沿最窄处宽度测量,按DDLS分级标准进行分级;Stratus OCT 3000成像仪、RNFL3.4程序获取象限位、钟点位及自选参数的视网膜神经纤维(retina nerve fiber layer,RNFL)厚度。运用SPSS15.0统计软件分析DDLS分级与OCT视盘周围3.4 mm处RNFL检测参数的相关性。结果共38例(73眼)中大直径视盘17眼(占23.3%),中等直径视盘41眼(占56.2%),小直径视盘15眼(占20.6%);上方、下方、颞侧象限RNFL厚度均值与DDLS分级评分相关(r=-0.673,P=0.0001;r=-0.605,P=0.0001,r=-0.499,P=0.0014),鼻侧方象限RNFL厚度均值与DDLS分级评分不相关(r=-0.352,P=0.0602);11、7、6、10、12钟点位RNFL厚度与DDLS分级评分具有相关性(r=-0.673,P=0.0001;r=-0.605,P=0.0001;r=-0.531,P=0.0006;r=-0.525,P=0.0007;r=-0.520,P=0.0008),而3、2、8点位无统计学意义(r=-0.320,P=0.0501;r=-0.320,P=0.0510;r=-0.297,P=0.0702);DDLS分级评分与OCT检查自选参数Avg、Smax、Imax的RNFL厚度也呈中度相关(r=-0.582,P=0.0001;r=-0.504,P=0.0012;r=-0.478,P=0.0024)。结论 DDLS分级方法对PCACG视神经损害的评估与OCT视网膜神经纤维厚度主要检查参数检查具有相关性,DDLS分级方法适用于PCACG视神经损害的评估。 相似文献
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《Pathology, research and practice》2019,215(10):152577
Aberrant expression of microRNAs (miRNAs) has been widely recognized to play critical roles in the pathogenic processes of colon cancer. However, the expression and functions of miR-3653 in colon cancer remain uncovered. This study revealed for the first time that miR-3653 expression was significantly decreased in colon cancer tissues and cell lines. MiR-3653 overexpression led to decreased migration and invasion of HCT116 cells while miR-3653 knockdown resulted in opposite influence of the metastatic behaviors of HT29 cells. qRT-PCR and western blot demonstrated that miR-3653 suppressed the epithelial-mesenchymal transition (EMT) of colon cancer cells using both gain- and loss- of function assay. Mechanically, miR-3653 was found to interact with the 3′-UTR of Zeb2 through the complementary sequences and inhibited the expression of Zeb2 in colon cancer cells. Rescue experiments demonstrated that the inhibitory effect of miR-3653 overexpression on cell metastasis and EMT was abrogated by forced expression of Zeb2. This study demonstrates that miR-3653 suppresses the metastasis and EMT of colon cells by targeting Zeb2, and serves as a promising biomarker and therapeutic target in colon cancer. 相似文献