小鼠匹鲁卡品致后海马活化素A、抑制素A蛋白及活化素ⅡA受体mRNA的差异表达

目的 :观察癫持续状态 (SE)后小鼠海马活化素A、抑制素A蛋白及活化素ⅡA受体mRNA表达的变化。方法 :采用匹鲁卡品诱导的SE小鼠模型 ,分别应用Westernblot与RT PCR观察SE后活化素A与抑制素A蛋白及活化素ⅡA受体mRNA表达的动态变化。结果 :①活化素A蛋白于SE后 3h开始增高 ,6h显著增高 ,2 4h至高峰 ,48h仍维持在较高水平 ;而未成SE的小鼠活化素A表达无明显变化。②抑制素A蛋白SE后无明显增加 ,同正常小鼠及未成SE的小鼠比较无显著差异。③活化素ⅡA受体mRNA在SE后表达无明显变化。结论 :SE后海马活化素A、抑制素A蛋白及活化素ⅡA受体mRNA表达存在差异性 ;活化素A可能对性脑损伤的保护与修复具有重要作用。     

中枢神经系统白血病血清神经元特异性烯醇酶测定的临床意义

目的 探讨血清神经元特异性烯醇酶与中枢神经系统白血病之间的关系。方法 对确诊的30例急性白血病患者及匹配的正常人的血清神经元特异性烯醇酶进行测定。结果 中枢神经系统白血病组血清神经元特异性烯醇酶含量21．92 7．5μg/L均明显高于白血病组、对照组，差异有显著性。结论 血清神经元特异性烯醇酶可作为反映中枢神经系统白血病病情的生化指标。     

血清HA、PCⅢ对慢性肝炎、肝硬化的诊断价值

目的 探讨血清ＨＡ、ＰＣⅢ对慢性肝炎,肝炎肝硬化的诊断价值,ＨＡ与ＰＣⅢ意相关关系以及它们的变化对预后的影响。方法 采用ＲＩＡ法同时检测慢性肝炎、肝炎肝硬化患者血清ＨＡ、ＰＣⅢ含量,比较ＨＡ,ＰＣⅢ间关系,肝炎肝硬化组中存活病例与死亡病例的ＨＡ、ＰＣⅢ变化情况。     

偶氮胂Ⅲ测定血清镁

目的 :建立简便、灵敏的偶氮胂Ⅲ (ArsenanoⅢ )血清镁测定法。方法 :在碱性条件下 ,用EGTA消除钙的干扰 ,偶氮胂Ⅲ与镁离子形成蓝紫色络合物 ,以分光光度法测定血清镁。结果 :偶氮胂Ⅲ -镁反应液的最大吸收峰为 6 15nm ,反应最适pH8 8,偶氮胂Ⅲ最适浓度为 15 0umol/L ,EGTA加入量为 2 0 0umol/L。本法批内CV及批间CV(n =2 0 )分别为 1 70～ 2 10 %、2 17～ 2 5 7%。平均回收率为 99 3% ,线性 0～ 3 0mmol/L。胆红素 (5 0 0umol/L以内 ) ,血红蛋白 (7g/L)。脂肪乳剂Interlipid(4g/L)对测定无干扰。该法与MTB法对照 ,结果无显著差异。 (P >0 0 5 )。结论 :偶氮胂Ⅲ测定血清镁简便、快速、灵敏、可靠 ,适于手工分析和自动化分析     

纤维状矿物粉尘对肺泡巨噬细胞膜损伤的机制研究

评价来自全国６个不同矿床的６种纤维矿物粉尘的细胞膜毒性,探讨其受损机制,采用体外细胞培养技术及扫描电镜方法观察纤维状矿物粉尘对肺泡巨噬细胞膜的通透性、膜流动性、膜形成的影响。结果纤维硅灰石,斜发沸石无细胞膜毒性,纤状海泡石、纤状坡缕石、纤状水镁石、纤状蛇纹石石棉表现出不同程度的细胞膜毒性。纤维状矿物粉尘对细胞膜毒性与其表面特性有关     

阿法替尼引起痤疮样药疹二例

阿法替尼属于表皮生长因子受体抑制剂,现广泛应用于非小细胞肺癌的治疗,该药物的主要毒性反应之一为皮肤不良反应,最常见为痤疮样药疹,本文报道2例服用阿法替尼后出现痤疮样药疹的患者。     

Effects of smoking intensity trajectory,cumulative smoking exposure,and the number of years since quitting on the subsequent risk of hypertension

The aim of this study was to investigate the effects of smoking intensity trajectory, cumulative smoking exposure, and the number of years since quitting on the subsequent risk of hypertension in Chinese adults. The study included 2783 men and 3416 women who participated in at least three waves of the China Health and Nutrition Survey. Information regarding smoking behavior was obtained using a standardized questionnaire. The trajectory of smoking intensity was determined using a group‐based trajectory model. The number of pack‐years of smoking and the number of years since quitting were calculated. The incidence of hypertension was 18.2% and 15.5% in men and women, respectively, during a median follow‐up duration of 4 years. Male participants with trajectories denoting light and moderate smoking had increased risks of hypertension compared with those with trajectories denoting non‐smoking (P_s  < .05). Compared with male participants with 0 pack‐years, those with < 5.5 pack‐years had a higher risk of hypertension (P < .05). Male participants with ≥5.5 pack‐years and weight gain had a higher risk of hypertension compared with those with 0 pack‐years and weight loss (P < .05). However, smoking was not related to an increased risk of hypertension in women. Additionally, similar to never smoking, having quit within 2–5 years or ≥5 years was not associated with the incidence of hypertension in men. The results of this study showed that light/moderate smoking or high cumulative smoking exposure accompanied by weight gain increased the risk of hypertension in Chinese men and smoking cessation decreased this effect.     

Rhodiola rosea polysaccharides promote the proliferation of bone marrow haematopoietic progenitor cells and stromal cells in mice with aplastic anaemia

ContextThe effects of Rhodiola rosea L. (Crassulaceae) polysaccharides (RRPs) on haematopoiesis are poorly understood.ObjectiveTo determine the effects of RRPs on haematopoiesis in mice with aplastic anaemia.Materials and methodsAplastic anaemia was induced in Kunming mice by ⁶⁰Coγ (2.0 Gy) irradiation and cyclophosphamide administration (50 mg/kg/day for 3 consecutive days; intraperitoneal injection). The in vivo effects of RRPs (10, 20, and 40 mg/kg; intraperitoneal injection) on haematopoiesis were analyzed using peripheral blood tests, histopathological examination of haematopoietic tissues, culture of haematopoietic progenitors and bone marrow stromal cells (BMSCs), and Western blotting of Fas and Fas ligand (FasL). The in vitro effects of RRPs on bone-marrow haematopoietic progenitors and BMSCs were also evaluated.ResultsCompared to anaemic controls, high-dose RRPs (40 mg/kg) significantly increased red blood cells (8.21 ± 0.57835 versus 6.13 ± 1.34623 × 10¹²/L), white blood cells (5.11 ± 1.6141 versus l.54 ± 1.1539 × 10⁹/L), and BMSCs (10.33 ± 1.5542 versus 5.87 ± 3.1567 × 10¹²/L) in mice with aplastic anaemia (all p < 0.01). High-dose RRPs significantly increased the formation of colony-forming unit-granulocyte macrophage (CFU-GM), burst-forming unit-erythroid (BFU-E), and colony-forming unit-erythroid (CFU-E; p < 0.01). Fas and FasL protein expression in BMSCs decreased after RRPs administration. Especially at the high dose, RRPs (150 μg/mL) significantly promoted in vitro CFUs-E, BFUs-E, and CFUs-GM formation. RRPs (150–300 μg/mL) also promoted BMSC proliferation.Discussion and conclusionsRRPs helped to promote haematopoietic recovery in mice with aplastic anaemia, facilitating haematopoietic tissue recovery. This study indicated some mechanisms of the haematopoietic regulatory effects of RRPs. Our findings provide a laboratory basis for clinical research on RRPs.     

Giant cervical goiter in Hashimoto’s thyroiditis: A case report

A giant cervical goiter, defined as a thyroid mass larger than 8 cm in diameter, is usually a nodular or adenomatous goiter. A giant cervical goiter can also be caused by hyperthyroidism (i.e., Hashimoto’s thyroiditis). The surgical indications for patients with Hashimoto’s disease include suspected malignant tumors, persistent symptoms related to the disease, or persistent enlargement of the goiter. We herein describe a woman who developed symptoms of compression from a thyroid tumor, the volume of which was almost the largest reported in the relevant literature to date. The bilateral lobes of the giant thyroid tumor were removed by total en bloc excision. We protected the bilateral recurrent laryngeal nerve and preserved the bilateral upper and lower parathyroid glands in situ. The excised left lobe tumor was 16 × 9 × 5.5 cm, whereas the right lobe tumor was 12 × 8 × 4 cm. The pathological diagnosis was Hashimoto’s thyroiditis. Although surgical excision is difficult, it is still the main treatment modality for giant goiters in patients with Hashimoto’s thyroiditis and can help to reduce the occurrence of complications.     

上尿路结石微创术后尿源性脓毒血症的危险因素研究进展

【摘要】 尿脓毒血症又称尿源性脓毒血症, 其病情凶险、起病隐匿、进展快、病死率高。近年来随着腔内泌尿微创技术在上尿路结石手术治疗中的广泛应用, 尿源性脓毒血症的发病数逐渐增多, 死亡率上升。从手术患者中筛选出具有发生尿源性脓毒血症高危因素的患者并给予重点关注和追踪, 对术后尿源性脓毒血症的早期诊断和治疗有着重要意义。现结合文献复习, 对上尿路结石微创术后并发尿源性脓毒血症的常见危险因素研究进展做一综述。