Evaluation of retinal vessel and perfusion density in migraine patients by optical coherence tomography angiography

BackgroundMigraine is a neurovascular disease and has been reported as a risk factor for ocular vascular complications. Our study aimed to compare the retinal vessel density and perfusion density between migraine patients and healthy subjects by optical coherence tomography angiography (OCTA).MethodsIn this prospective study, 23 patients with migraine with aura (MWA) and 31 patients with migraine without aura (MWOA), and 32 age- and gender-matched healthy controls (HC) were enrolled. The vessel density (VD) and perfusion density (PD) were evaluated in a 6 × 6 mm scan of the macula and optic nerve head (ONH) with the Cirrus HD-OCT 5000 device. The measurement area is divided into three areas: center (c), inner ring (ir), outer ring (or) (with diameters of 1, 3, and 6 mm respectively), and nine subfields, according to the Early Treatment Retinopathy Study grid.ResultsThe macular cVD, cPD, and temporal orVD in MWA and MWOA groups were significantly reduced than those of HC. On optic nerve head OCTA, patients with MWA had decreased cVD, average irVD, inferior irVD, and temporal orVD compared with HCs while MWOA had reduced cVD than HC group. In addition, PD was not significantly different among MWA, MWOA, and HC groups in any measure in the optic nerve head. The Migraine Disability Assessment Score (MIDAS) and attack frequency were significantly inversely correlated with cVD, cPD, irVD, and irPD of macula and ONH.ConclusionsVessel and perfusion density of macula were reduced in both MWA and MWOA. Vessel density, but not perfusion density of ONH was decreased in MWA. The migraine severity and attack frequency were significantly inversely correlated with vessel and perfusion density of macula and ONH.     

丹红注射液结合抗菌药物对腹股沟疝术后血清MMP-2、MMP-9、TIMP-2、TIMP-1及应激指标作用机制研究

目的:研究丹红注射液结合抗菌药物对腹股沟疝术后血清MMP-2、MMP-9、TIMP-2、TIMP-1及应激指标作用。方法:回顾性分析选取的2015年1月至2017年11月河北北方学院附属第一医院收治的绞窄性疝患者152例作为研究对象,按照术后不同的用药方法分为观察组(n=75)和对照组(n=77)。在腹股沟疝手术后,对照组患者单纯给予静脉注射常规抗菌药物。观察组在对照组患者的基础上额外增加丹红注射液。记录2组包括皮质醇(Cor)、去甲肾上腺素(NE)、醛固酮(ALD)等应激指标的变化幅度,以及血清MMP-2、MMP-9、TIMP-2、TIMP-1的水平比较。结果:观察组术后应激指标Cor,NE,和ALD均显著优于对照组;观察组检测后血清MMP-2、MMP-9、TIMP-2、TIMP-1水平,与对照组患者比较,差异有统计学意义(P 0. 05);观察组检测后炎性反应因子水平IFN-γ、TNF-α、IL-2、IL-4、IL-6、IL-10水平,与对照组患者比较,差异有统计学意义(P 0. 05)。结论:丹红注射液结合抗菌药物对腹股沟疝术后恢复疗效显著,比起单纯使用抗菌药物,血清MMP-2、MMP-9、TIMP-2、TIMP-1水平、应激指标与炎性反应因子均有明显优势,值得临床广泛应用。     

妊娠期糖尿病母亲新生儿血清蛋白质、血脂、免疫功能的变化及临床意义

目的 探讨妊娠期糖尿病(GDM)母亲新生儿血清蛋白质、血脂、免疫功能的变化及临床意义。方法 选择2020年7月至2021年12月收治的108例诊断为GDM母亲新生儿作为研究对象,另选择同期80例正常母亲新生儿作为对照组。所有新生儿均于出生后24 h时采集空腹外周静脉血5 mL,检测两组新生儿的血清蛋白质[总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、前白蛋白(PA)],血脂水平{脂蛋白a[Lp(a)]、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)}和血清免疫球蛋白(Ig)G、IgM、IgA、补体C3、补体C4水平,以及外周血自然杀伤(NK)细胞比例和T淋巴细胞亚群(包括CD3⁺、CD4⁺、CD8⁺T细胞比例),并计算CD4⁺T细胞与CD8⁺T细胞比值(CD4⁺/CD8⁺)。结果 观察组新生儿血清TP、ALB、GLB和PA水平均显著低于对照组新生儿(P<0.05)。观...     

皮层扩散性抑制的研究进展

皮质扩散性抑制（cortical spreading depression，CSD）是大脑皮质受刺激后产生的电活动抑制带，该抑 制带可以将大脑皮质跨膜离子的浓度梯度迅速摧毁并去极化，导致细胞内外发生短路，可以影响大脑的灌注及 代谢，导致不良预后，该文将从以下几个方面对CSD 的研究进展进行综述。     

宫颈上皮内瘤变锥切术后HPV检测的阴性预测价值

目的评定宫颈上皮内瘤变锥切术后高危型人乳头瘤病毒(HPV)检测的阴性预测价值。方法收集宫颈上皮内瘤变CINⅡ～CINⅢ的110患者并进行回顾性分析,宫颈锥切后定期行液基细胞学宫颈TCT及宫颈HPV检测,对子宫颈锥切术后切缘状态(切缘阳性)和高危型HPV状态与随后切除的子宫标本的残留病变进行相关性比较。高危型HPV检测应用二代杂交捕获(HC2)分析。结果在分析的110例患者中,47例(42.7%)宫颈锥切术后切缘为阳性,其中37例(78.7%)患者锥切后高危型HPV阳性,10例(21.3%)HPV阴性。这47例中,31例(66.0%)患者锥切后切除的子宫标本中有残留病变。宫颈锥切后63例(57.3%)切缘阴性的患者中,49例(77.8%)患者高危型HPV阴性患者均无残留病变,14例(22.2%)阳性患者中4例(6.3%)有残留病变。结论宫颈锥切术后进行HPV DNA检测具有很高的阴性预测价值。与之相伴的优点是能够预测CIN宫颈锥切患者术后残存病变的发生或复发。     

The oxidative stress,mitochondrial pathway apoptosis,and the antagonistic effects of chrysophanol in SH-SY5Y cells via DTPP-induced photodynamic therapy

BackgroundTo investigate the susceptibility of SH-SY5Y cells to DTPP-based photodynamic therapy (PDT) and the antagonistic effects of chrysophanol (Chr) on PDT.MethodsPDT photocytotoxicity to cells was quantified and determined by exposing increasing concentrations of DTPP between 2.5 to 20 μg/mL to radiation with energy densities of 1.2–9.6 J/cm² at 630-nm wavelength. Sodium azide (SA, NaN₃) and d-mannitol (DM) were employed to study the reaction type of PDT. The photodynamic stress after PDT was assessed by superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidative capacity (T-AOC) assays. The apoptosis pathway of SH-SY5Y cells after PDT was studied by the determination of JC-1 and caspase-9/Caspase-3 concentrations. MTT and double fluorescence staining assays were applied to study the effect of Chr on cell survival and apoptosis rate in PDT, respectively. PI was used to detect the effect of Chr on cell membrane integrity after DTPP-PDT treatment.ResultsThe dose-dependent killing effect of high DTPP concentrations and irradiation doses were identified. Cell apoptosis is mediated by a mitochondrial pathway with a total apoptosis rate of 33.8% at 10 μg/mL of DTPP after irradiation with 2.4 J/cm². Oxidative stress was produced by ROS in PDT and non-reversible cell oxidative damage appeared due to the cells’ modulation of the oxidative stress balance during the PDT response. Chr had a- effect on ROS capture and an inhibitory effect on the PDT-induced destruction of cell membranes.ConclusionsSH-SY5Y cells were susceptible to DTPP-PDT, resulting in a mitochondrial apoptosis pathway. There is an antagonistic effect of Chr on PDT in SH-SY5Y neuroblastoma cells.     

超声诊断胃平滑肌瘤38例分析

目的 探讨胃平滑肌瘤在胃充盈超声下的声像图特征.方法 回顾性分析38例经病理证实平滑肌瘤在胃充盈超声检查中的超声影像资料.结果 特征表现为：黏膜下圆形、类圆形,实性,低回声,边界清楚,回声均匀肿物;肿瘤内少许血流,多见于胃底、体部;大小0.5～5.0 cm,与周围组织界限清楚.胃充盈超声技术对胃平滑肌瘤的诊断符合率85.2%.结论 胃部平滑肌瘤在胃充盈超声下具有特征性,胃充盈超声技术对胃平滑肌瘤诊断有一定价值.     

康脑液1号对大鼠脑缺血再灌注病理学改变及神经细胞凋亡的影响

目的观察康脑液1号对SD大鼠脑缺血再灌注后组织病理学改变及神经细胞凋亡的影响,探讨其对脑缺血再灌注保护作用的可能机制。方法采用栓线法复制SD大鼠大脑中动脉梗死模型（MCAO）,分别于缺血2h后再灌注6、24、72h。将150只大鼠随机分为5组,每组30只,假手术组、模型（缺血再灌注）组、盐酸法舒地尔组、康脑液1号A组、康脑液1号B组。观察康脑液1号对大鼠脑缺血再灌注神经功能、脑梗死面积和病理形态学的影响。采用TUNEL凋亡法检测分析脑缺血半暗带和灶中心区凋亡阳性细胞的数目。结果模型组与假手术组比较,凋亡细胞阳性数目增多;康脑液1号可不同程度地降低实验性脑缺血大鼠的神经功能评分、脑梗死面积及减轻脑组织病理形态改变（P〈0.05）;康脑液1号2组大鼠梗死灶面积减小,脑组织缺血半暗带凋亡细胞阳性数目减少,而梗死灶中心凋亡细胞阳性数目增多;康脑液1号A组与康脑液1号B组比较差异无统计学意义（P〉0.05）。结论康脑液1号可能通过调节脑缺血再灌注后神经细胞凋亡而促进脑损伤修复及重塑,从而发挥脑保护作用。