Effects of Peroxisome Proliferator-Activated Receptor-γ Activation on Apoptosis in Rats with Acute Pancreatitis

Purpose

To investigate the effects and mechanisms of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation on the induction of apoptosis in rats with acute pancreatitis.

Methods

Severe acute pancreatitis (SAP) and mild acute pancreatitis (MAP) were induced and pre-treated with pioglitazone, which is a ligand of PPAR-γ. The expression of inflammatory factors (TNF-α and IL6) of the pancreas was detected by ELISA. The apoptosis in pancreas were detected by TUNEL assay and the activity of caspase 3 was determined. Phosphorylation of p65 in pancreas of SAP or MAP was determined by western-blot.

Results

Expression levels of PPAR-γ proteins were elevated in the pancreases of SAP or MAP rats pre-injected with pioglitazone intraperitoneally. Downregulation of the expression TNF-α and IL6 and relief of pathological changes in the pancreas suggested that pioglitazone had protective effects on acute panceatitis. In pioglitazone pre-treated groups, a TUNEL assay indicated a high level of apoptosis in SAP but little apoptosis in MAP, showing pioglitazone could promote taurocholate-induced apoptosis but inhibit ceruleininduced apoptosis in pancraeatic aniniar cells. Furthermore, caspase 3 activity was high in SAP but low in MAP, implying that the apoptotic mechanism in pancreatic acinar cells of AP rats was correlated with caspase 3 activity. Phosphorylation of p65 was reduced in SAP or MAP group pretreated with pioglitazone, indicating that pioglitazone reduced the inflammation reaction by inhibiting the activation of the NF-κB.

Conclusions

These results indicated that activation of PPAR-γ induced apoptosis in pancreatic acinar cells of SAP rats but inhibited apoptosis in pancraeatic acinar cells of MAP rats, which demonstrated that PPAR-γ may be an efficiently therapeutic target in pancreatic inflammation.     

中国上海汉族人群NAD(P)H氧化酶p22phox亚基C242T基因多态性与脑卒中相关性

目的:研究NAD(P)H氧化酶p22phox亚基C242T基因多态性与脑卒中的相关性?方法:收集118例脑出血患者?125例脑梗死患者和147例正常对照组,研究对象均来自上海地区汉族人群,3组在年龄?性别构成比?体质指数(BMI)等基线指标均没有明显差异,分别进行血糖?血脂等各项指标的测定,用聚合酶链反应?限制性片段长度多态性分析(PCR－RFLP)和基因测序方法比较其在不同人群中的基因突变频率?结果:脑出血组?脑梗死组CT + TT基因型(9.3%?9.6%)和T等位基因(4.7%?5.2%),均明显高于对照组(2.0%和1.0%,P < 0.05),脑梗死组中发现1例TT基因型,出血组与正常对照组均未发现TT基因型,与基因测序结果一致?采用二分类Logistic回归分析p22phox亚基C242T基因多态性与脑出血的相关性(β = 1.712,OR = 5.537,95%CI:1.120~27.381,P = 0.036),与脑梗死的相关性(β = 1.432,OR = 4.187,95%CI:0.934~18.774,P = 0.061),表明C242T基因多态性可能是脑出血?脑梗死的危险因素?结论:NAD(P)H氧化酶p22phox亚基C242T多态性与脑出血?脑梗死均有一定的相关性,可能是脑卒中的一个危险因素?但与高血压和饮酒等传统危险因素相比,C242T多态性对脑梗死的影响并不显著?     

方松街道70岁以上老人代谢异常调查分析

目的:了解上海市松江区方松街道70岁以上老年人代谢异常患病情况,为社区卫生服务提供依据。方法:对方松街道70岁以上老年人进行健康问卷调查和体检,检测身高、体重、血糖、血脂、血压等,对结果进行统计分析。结果:参与体检的老年人代谢性疾病总患病率为79.51%,高血压、血脂异常、高血糖、肥胖患病率分别为64.75%、36.59%、17.56%、24.12%。不同性别的老年人在高血压、高血糖、肥胖患病率方面差异无统计学意义(P>0.05)。女性血脂异常患病率明显大于男性(P<0.01)。随着体质指数的增加,高血压、血脂异常、高血糖患病率呈现上升趋势(P<0.01)。结论:社区老年人群是代谢性疾病的高发人群,其患病率随体质指数的增加而增加。     

毛细管气相色谱法测定糜蛋白酶中磷酸三丁酯残留量

目的建立用于检测糜蛋白酶原料药中磷酸三丁酯残留量的毛细管气相色谱法。方法供试品采用正己烷提取,内标法测定。TM—FFAP毛细管柱,载气为高纯氮气,正己烷为溶解介质,柱温为恒温160℃,进样口温度为200℃,检测器温度为230℃,进样量1．0μL,分流进样,分流比1：5,FID检测器。结果被测物磷酸三丁酯在所考察的浓度范围内呈现良好线性关系,r=0．9966,平均回收率为96．28％～103．54％,精密度的RSD〈5％,完全符合残留溶剂测定的技术要求。结论本试验建立的方法简单、准确、精密度高,可用于糜蛋白酶中磷酸三丁酯残留量的测定。     

Paramètres physiologiques à l'exercice sous-maximal aérobie : interaction entre sexe et entraı&#x0302;nement

Objective – Examine possible gender differences in cardiocirculatory responses during submaximal exercise, after an endurance training program.Methods and results – Oxygen uptake and heart rate were determined at differently submaximal work loads (35; 52.5; 70 Watts) before and after a 13 week endurance training program. The present longitudinal study shows a decrease in heart rate at submaximal work loadin prepubertal children after an aerobic training program and that such a decrease is similar in boys and girls.     

Pioglitazone: A Promising Therapeutic Tool in Sodium Taurocholate-Induced Severe Acute Pancreatitis

Background  

Studies suggest that peroxisome proliferator-activated receptor γ(PPARγ) ligands may represent a therapeutic option in acute pancreatitis, yet most of them have been prophylactic administrated.     

六种自身免疫性抗体联合检测在早期类风湿关节炎诊断中的临床意义

目的：研究类风湿因子（RF）、隐性类风湿因子（HRF）（RFIgA、RFIgG、RFIgM）,抗角蛋白抗体（AKA）,抗环瓜氨酸肽抗体（抗CCP抗体）的联合检测对类风湿关节炎（RA）的早期诊断价值。方法：采用比浊法检测RF,采用ELISA法定量检测HRF,采用ELISA法检测抗CCP抗体,间接免疫荧光法检测AKA。回顾性分析了RA患者301例,其中3、12、24个月以内的早期RA患者分别为62、142和171例;非RA的风湿病患者125例。通过病程分期把RA分成早期（病程24个月之内）和中晚期患者（病程24个月以上）,比较早期RA各因子之间的敏感性、特异性及联合检测的结果。结果：病程≤3个月分别与病程≤12个月、≤24个月各项诊断指标敏感性比较,差异无统计学意义（P〉0.05）。特异性由高到低依次为AKA（97.37%）、抗CCP（92.24%）、RFIgG（90.76%）、RFIgM（82.20%）、RFIgA（77.31%）、RF（76.71%）。联合检测,RF（-）的RA患者,AKA阳性率达22.50%（9/40）,抗CCP抗体阳性率达57.58%（19/33）,HRF阳性率达55.00%（22/40）,其他5项中1项以上阳性率达50.00%（9/18）;RF（＋）者其他5项中1项以上阳性率达89.74%（70/78）;RF（-）抗CCP抗体（-）者其他4项中1项以上阳性率达23.81%（5/21）。结论：6种自身抗体均可出现在早期的RA患者,联合检测对RA早期诊断有较高的价值,其中AKA、抗CCP抗体对RA有高度的特异性,且在RA早期即可出现。     

我院危重病学科护理人员集束护理依从性的调查研究

目的了解危重病学科护理人员对呼吸机相关性肺炎集束化治疗的护理依从性,探讨其影响因素和改善措施。方法根据呼吸机相关性肺炎的主要临床预防措施自行设计调查表,对危重病学科的64名在职护理人员进行问卷调查。结果护理人员的集束护理平均依从性为80．85％,在7个护理项目中洗手的依从性最差为72．34％。护理人员自认为依从性低的因素主要是觉得重要但没有时间、觉得不重要、懒得做和疾病限制以及患者因素。结论应改善目前护理人员配置,加强集束护理相关教育培训,增强护理人员的主观护理意识和慎独精神,从而提高集束护理依从性。     

预真空压力蒸汽灭菌效果监测不达标原因分析及探讨

压力蒸汽灭菌是目前热力灭菌中最普遍、效果最理想的一种灭菌方法,其优点是蒸汽穿透力强、灭菌效果可靠、能杀灭所有微生物。通过仔细观察故障现象,结合透彻理解压力蒸汽灭菌原理,巧妙利用监测结果,可以更快速准确分析问题解决故障。