鼻内镜术中不同中鼻甲处理方式治疗慢性鼻-鼻窦炎伴鼻息肉效果比较

探讨慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）患者鼻内镜术中不同中鼻甲处理方式的临床效果。     

MiR-1301-3p inhibits human breast cancer cell proliferation by regulating cell cycle progression and apoptosis through directly targeting ICT1

Background

MiRNAs regulate a variety of biological processes, such as cell proliferation and apoptosis and play critical roles in cancer progression. Accumulating studies have demonstrated that miR-1301-3p could regulate the development and progression of multiple cancers, but its biological behaviors in breast cancer (BC) are still elusive.

Methods

The expression of miR-1301-3p was determined in BC tissues and cell lines using quantitative real-time PCR analysis. The effects of miR-1301-3p on BC cell growth, proliferation, cell cycle distribution, and apoptosis were also explored in vitro using MTT, colony formation and Flow cytometry assays. The potential target gene of miR-1301-3p was determined by dual-luciferase reporter assay and verified by quantitative real-time PCR and western blot analysis.

Results

We found the expression of miR-1301-3p was observably significantly down-regulated in BC tissues and cell lines. MiR-1301-3p expression in BC tissues was significantly associated with tumor size and clinical stage. Gain-of-function assays demonstrated that miR-1301-3p inhibited the cell growth and proliferation in breast cancer cell lines, MCF-7 and T-47D. Moreover, up-regulation of miR-1301-3p induced cell cycle G0/G1 phase arrest and apoptosis. Mechanistically, up-regulation of miR-1301-3p reduced the expression of CDK4, Cyclin D1, Bcl-2, but elevated the expression of p21, Bad and Bax. ICT1 was confirmed as a direct target of miR-1301-3p. Furthermore, ICT1 overexpression could partially reverse the effects of miR-1301-3p on BC cell proliferation, cell cycle progression and apoptosis.

Conclusion

Our observations suggested that miR-1301-3p inhibits cell proliferation via inducing cell cycle arrest and apoptosis through targeting ICT1, and might be a therapeutic target for BC.

     

CT 窗口技术在肺磨玻璃结节诊断中的临床意义

目的 探讨肺腺癌磨玻璃结节（GGN）术前薄层胸部CT 图像中,不同窗宽窗位下测得的GGN 大小对其病理浸润性的预测价值。方法 回顾性分析2015 年1 月—2018 年3 月复旦大学附属中山医院青浦 分院手术证实为肺腺癌的47 例患者,共49 个GGN 术前薄层胸部CT 图像及术后病理结果。测量GGN 在肺 窗（窗宽1 500 HU,窗位-400 HU）、纵隔窗（窗宽350 HU,窗位50 HU）及调整窗（窗宽1 300 HU,窗位 50 HU）的平均直径并结合术后病理分析。结果 49 个GGN 中浸润性腺癌（IAC）32 个,非浸润性腺癌17 个;后者含13 个微润浸腺癌（MIA）,2 个原位癌（AIS）,2 个不典型腺瘤样增生。肺窗上GGN 平均直径以 15 mm 为临界值,调整窗和纵隔窗上GGN 平均直径以5 mm 为临界值区分IAC 和非IAC ;GGN 平均直径≥ 临界值组的肺腺癌为浸润性腺癌的比例升高（P <0.05）。以肺窗上测得的GGN 平均直径≥ 15 mm 诊断的肺 腺癌为IAC 的敏感性为62.50%,特异性为88.24%。以调整窗和纵隔窗上测得的GGN 平均直径≥ 5 mm 诊断 的肺腺癌为IAC 的敏感性分别为75.00% 和31.25%、特异性分别为94.12%、100.0%。结论 不同窗宽窗位下 测得的GGN 大小与浸润性相关。综合考虑不同窗宽窗位测得的GGN 大小对GGN 肺腺癌是否浸润有预测 价值,为术前制定手术方案提供参考。     

间歇充气加压与低分子肝素预防内科ICU高危病人下肢DVT的临床观察

目的探讨间歇充气加压（IPC）与低分子肝素（LMWH）预防内科高危病人下肢深静脉血栓（DVT）的效果和安全性。方法选取静脉血栓栓塞高风险病人100例,随机分为4组:IPC组、LMWH组、IPC+LMWH组和空白对照组。于治疗前及治疗后第1、4、7天,行下肢彩色多谱勒检查,观察各组腘静脉和股静脉的血流速度,判断是否存在血栓,同时检测活化部分凝血活酶时间（APTT）、血栓弹力图,比较各组发生下肢DVT的例数及不良事件。结果治疗前各治疗组与空白对照组APTT、血栓弹力图各指标差异均无统计学意义（P>0.05）,治疗后各组APTT、血栓弹力图各指标与治疗前之间的差异均无统计学意义（P>0.05）,治疗后各治疗组与空白对照组比较,APTT、血栓弹力图各指标的差异均无统计学意义（P>0.05）。各组治疗前腘静脉、股静脉平均血流速度差异均无统计学意义（P>0.05）;与治疗前相比,IPC组及IPC+LMWH组平均血流速度增快（P < 0.01）,LMWH组与对照组平均血流速度均无明显变化（P>0.05）。IPC组发生下肢DVT 2例（8%）,LMWH组2例（8%）,IPC+LMWH组1例（4%）,空白对照组8例（32%）,各治疗组DVT发生率均低于对照组（P < 0.05~P < 0.01）。各组均未发生出血不良反应。结论应用IPC与低分子肝素是安全、有效地预防内科高危病人下肢DVT的方法。     

Clinical observation on tuina treatment for cervical intervertebral disc herniation

目的：观察推拿治疗颈椎间盘突出症的临床疗效。方法：87例患者根据就诊顺序随机分为治疗组和对照组。治疗纽39例,采用推拿手法治疗;对照纽48例,采用颈椎牵引结合中频脉冲治疗。两组均每星期治疗3次,治疗10次为一个疗程。结果：治疗组治愈22例,显效13例,好转4例,显效率89．7％,总有效率100．0％;对照纽治愈23例,显效17例,有效7例,无效1例。显效率83．3％,总有效率97．9％。两组显效率及总有效率比较,差异均无统计学意义。治疗组平均治疗17．8次,对照组平均治疗26．6次。结论：推拿治疗颈椎间盘突出症疗效与牵引结合中频脉冲治疗相当,但推拿治疗具有见效快,疗程短的优势。     

Efficacy of zinc given as an adjunct to the treatment of severe pneumonia: A meta‐analysis of randomized,double‐blind and placebo‐controlled trials

Background

Pneumonia is a major cause of morbidity and mortality of children. Zinc is known to play a central role in the immune system. The deficiency of zinc increased susceptibility to infectious diseases.

Objective

To investigate the clinical efficacy of zinc given as an adjunct therapy to the treatment of severe pneumonia.

Methods

The PubMed, Embase, MEDLINE^TM and the Cochrane Central Register of Controlled Trials were searched to identify all randomized, double‐blind and placebo‐controlled (DBPC) trials which evaluated the clinical efficacy of zinc given as an adjunct in the treatment of severe pneumonia and published between January 1966 and October 2015.

Results

Six randomized DBPC trials including 2216 patients with severe pneumonia were eligible. The results suggested that zinc given as an adjunct therapy to the treatment of severe pneumonia had no significant improvement of treatment failure (RR = 0.97, P = .71) and change of antibiotic therapy (RR = 1.09, P = .52). We also found a favorable trend for clinical deterioration of severe pneumonia but with no statistical significance (RR = 0.88, P = .55). Zinc produced a significant reduction in mortality caused by severe pneumonia (RR = 0.43, P = .01).

Conclusions

Zinc given as an adjunct to the treatment of severe pneumonia is effective in reducing the mortality of severe pneumonia, and has no significant effects on treatment failure and change of antibiotic therapy.     

腹腔镜胆囊切除术治疗急性结石性胆囊炎的体会

目的总结腹腔镜胆囊切除术（LC）治疗急性结石性胆囊炎的临床经验和手术操作技巧。方法回顾性分析我院2006年9月～2010年7月行LC的103例急性结石性胆囊炎患者的临床资料。结果 LC成功率91.26%（94/103）,中转开腹率8.74%（9/103）,并发症发生率1.94%（2/103）。结论急性结石性胆囊炎行LC是安全可行的,正确处理胆囊三角是成功的关键,适时中转开腹是手术安全的保障。     

C肽与2型糖尿病视网膜病变的相关性

目的探讨2型糖尿病(T2DM)患者C肽与糖尿病视网膜病变(DR)的相关性。方法T2DM患者150例分为有DR(A组,56例)和无DR(B组,94例)两组,比较两组空腹C肽、餐后2-hC肽,并对DR的危险因素作Logistic回归分析。结果 A组空腹C肽和餐后2-h C肽均低于B组(P<0.05或P<0.01)。餐后2-h C肽是DR发生的重要影响因素。结论合并DR的T2DM患者餐后2-h C肽降低是DR发生的危险因素。