Wogonin suppresses inflammatory response and maintains intestinal barrier function via TLR4-MyD88-TAK1-mediated NF-κB pathway in vitro

Inflammation Research - Wogonin has multiple pharmacological effects, including anti-inflammatory effects. Here, we hypothesize that wogonin can protect intestinal barrier function in...     

Occlusion de l’aorte abdominale chez les patients infectés par le virus de l’immunodéficience humaine : cas clinique et revue de littérature

HIV infection has now become a chronic disease with a good life expectancy thanks to antiretrovirals. The mortality currently is attributed to other pathologies in particular cardiovascular because of the inflammation and the side effects of the drugs. All arteries can be damaged in HIV, especially the aorta, with several types of lesions which can be occlusive, aneurysmal, dissecting, even with the cases of arteriovenous fistula which have been described. HIV occlusive arterial disease is different from atheromatous disease in HIV-free patients and this is confirmed by pathology and ultrasound studies, which makes it more difficult to manage HIV-related occlusions. The open surgical treatment especially in the acute forms is disappointing with complications of rethrombosis and infectious and of sepsis of prosthesis considering the immunosuppression, the endovascular treatment begins to become the treatment of choice in the aneurysmal pathology and probably it would be in the future for occlusive disease.     

The Study on the Efficacy of Fibrin Glue in Preventing Post-traumatic Focal Pancreatitis (PTFP) After Radical Gastrectomy

Objective

This study was intended to evaluate the efficacy of fibrin glue (FG) in preventing post-traumatic focal pancreatitis (PTFP) after radical gastrectomy by examining the drainage fluids over 7 days post-op.

Methods

Ninety-five patients who underwent D2 radical gastrectomy for gastric cancer were randomly assigned to a fibrin glue group (n?=?48) receiving fibrin glue on the raw surface of the pancreas during surgery and a control group (n?=?47), which did not receive fibrin glue.

Results

We found no significant difference in operation time and intraoperative blood loss between groups (p?>?0.05); no deaths occurred during surgery. The volume of ascitic fluid containing blood cells in the fibrin glue group was significantly lower than that in the control group (p?<?0.001) at all times observed. Amylase levels in the drained fluids were highest at 24 h postoperatively in both groups, suggesting pancreatitis, but gradually decreased to normal levels within 7 days. The amylase in the drains in the control group was significantly higher than that in the FG group (p?<?0.001) at all times observed, but it returned to normal 72 h postoperatively in the FG group. One death by hemorrhagic shock associated with PTFP was recorded in the control group.

Conclusion

Fibrin glue is safe and effective in preventing PTFP following gastric surgery and shortens the clinical course of the disease.     

Intravenous butorphanol administration reduces intrathecal morphine-induced pruritus after cesarean delivery: a randomized, double-blind, placebo-controlled study

Purpose

Pruritus associated with intrathecal opioid administration is a common side effect. There is evidence that κ-opioid receptor agonists have antipruritic activity. Butorphanol has agonist actions at both κ-opioid and μ-opioid receptors. This study was designed to evaluate the antipruritic efficacy of butorphanol after intrathecal morphine administration in the setting of a randomized, double-blind study of parturients undergoing cesarean section.

Methods

Ninety-one women who received combined spinal–epidural anesthesia with 1.2?ml 0.5?% isobaric bupivacaine and 0.1?mg preservative-free morphine were included in this study. After delivery of the baby, the parturients were randomly allocated to two groups: butorphanol group (n?=?46) and physiological saline group (n?=?45). In the butorphanol group, parturients received an intravenous loading dose of 1?mg butorphanol followed by infusion of 0.2?mg/h butorphanol. The physiological saline group received an infusion of the same volume of physiological saline. The presence of pruritus, visual analog scores for pain, sedation scores, and adverse effects were recorded 1, 2, 4, 6, 8, 10, 12, and 24?h after intrathecal morphine administration.

Results

The incidence of pruritus at 24?h was significantly more frequent in the physiological saline group than in the butorphanol group (48.9 vs. 13.0?%, P?<?0.001). The severity of pruritus was significantly greater in the saline group than in the butorphanol group 2, 4, 6, 8 and 10?h after intrathecal morphine injection (P?=?0.004, 0.001, 0.002, and 0.003, respectively). The visual analog scale scores at 24?h were significantly lower in the butorphanol group than in physiological saline group (P?<?0.001). The Ramsay sedation score in the butorphanol group was significantly higher than that in the physiological saline group after 1, 2, 4, 6, 8, 10, 12, and 24?h (P?<?0.05). There were no significant differences between the two groups in nausea/vomiting and other adverse effects.

Conclusion

Administration of intravenous butorphanol after delivery of the baby can reduce pruritus that has been induced by intrathecal morphine administration in cesarean delivery with combined spinal–epidural anesthesia.     

EV71-IgM抗体阳性的手足口病患儿免疫球蛋白血糖和脑脊液检测结果分析

目的分析肠道病毒71型(enterovirus 71,EV71)抗体阳性的手足口病患儿免疫球蛋白、血糖、脑脊液的检测结果,探讨其临床意义。方法选取胶体金法检测EV71-IgM阳性、阴性的手足口病患儿各50例,行血清免疫球蛋白、血糖及脑脊液检查,采用SPSS 12.5软件统计分析2组病例各项实验指标的差异。结果 50例EV71抗体阳性组患儿血清免疫球蛋白IgA、IgG、IgM分别为(0.52±0.38)、(8.36±2.15)和(1.52±0.26)g/L,50例EV71抗体阴性组患儿血清免疫球蛋白IgA、IgG、IgM分别为(0.41±0.21)、(8.45±2.34)和(1.48±0.27)g/L,2组比较,差异无统计学意义(P0.05)。50例EV71抗体阳性患儿中有26例血糖升高,血糖升高率为52.0%;22例脑脊液检查异常,脑脊液检查异常率为44.0%。而50例EV71抗体阴性组患儿中有4例血糖升高,血糖升高率为8.0%;3例脑脊液检查异常,脑脊液检查异常率为6.0%。2组比较,差异均有统计学意义(均P0.05)。结论在手足口病患儿中开展EV71抗体的检测同时检测患儿血糖和脑脊液有助于危重患儿的早期诊断和治疗。     

Characterization of the contribution of buccal absorption to internal exposure to bisphenol A through the diet

The gavage route is often used for the toxicological evaluation of food contaminants. This route does not take into account absorption of the toxicants through the buccal mucosa, as evidenced in dogs for bisphenol A (BPA). Our goal was to determine the functional significance of buccal BPA absorption during dietary exposure. Four ewes received BPA by nasogastric gavage (100 mg/kg) and through food pellets (10 mg/kg), 13 days apart. The time course of serum concentrations of BPA and its main metabolite BPA-G was submitted to non-compartmental analysis. The dietary route led to 3-fold higher bioavailability as compared to gavage. The ratio of BPA-G to BPA concentrations varied greatly over time after the food administration, but not after gavage, suggesting a delayed metabolism of BPA after dietary exposure. The maximum entrance rate of BPA in the systemic circulation, determined by deconvolution analysis, was much higher after dietary administration than after gavage and a biphasic pattern of BPA entry was observed in 3 of the 4 ewes. Our results evidenced a dual mechanism of BPA absorption (buccal and digestive) after dietary exposure and highlight the necessity to take buccal absorption into account when evaluating food contaminants.     

Wls Expression Correlates with Tumor Differentiation and TNM Stage in Hepatocellular Carcinoma

Background

Hepatocellular carcinoma (HCC) is an aggressive cancer with a poor prognosis. Effective biomarkers are necessary to predict the clinical course and outcome of patients with HCC. Wntless (Wls) is a key modulator of Wnt protein secretion and is overexpressed in various human cancers. However, the mechanism and alteration of Wls expression in HCC have not been clarified.

Aims

The aim of this study was to evaluate expression level of Wls in HCC and its clinical significance.

Methods

The levels of Wls expression were investigated in 84 HCC tissues using immunohistochemistry.

Results

Wls was negatively expressed in normal liver tissue and was negatively or weakly (score 0) expressed in liver cirrhosis. Twenty-eight out of 84 samples (33.3%) were negative or weakly (score 0) expressed Wls, 38 out of 84 (45.2%) moderately (1+) expressed Wls, and 18 out of 84 (21.4%) strongly (2+) expressed Wls. Wls expression was positively associated with tumor size (P = 0.005, r = 0.302), tumor TNM stage (P = 0.017, r = 0.261), AFP (P = 0.051), and HBV infection (P = 0.009, r = 0.283), and was negatively associated with differentiation (P < 0.001, r = ? 0.552). No significant relationship between Wls expression and liver cirrhosis, ALT, GGT, age, sex, or tumor focality was found.

Conclusions

Our data showed that Wls was differentially expressed in HCC. Statistical analysis results suggest that Wls expression might increase as HCC progresses.

     

Association of Kir6.2 gene rs5219 variation with type 2 diabetes: A meta-analysis of 21,464 individuals

Aims

rs5219 is in Potassium inwardly-rectifying channel, subfamily J, member 11 (KCNJ11) E23K gene, located at 11p15.1. Researches on the association between rs5219 gene polymorphism with type 2 diabetes mellitus (T2DM) were performed extensively, but the results remain controversial. To investigate the relationship, a meta-analysis involving 21,464 individuals was conducted.

INSM1在肺神经内分泌肿瘤活检标本和细胞学样本中的表达

目的:分析胰岛素瘤相关蛋白-1 (insulinoma-associated protein 1,INSM1)在肺神经内分泌肿瘤组织学和细胞学样本中的表达。方法:采用免疫组织化学En Vision两步法检测INSM1在193份活体组织检查(以下简称活检)标本及80份细胞学标本中的表达,其中包括:肺小细胞癌(42份)、肺大细胞神经内分泌癌(6份)、低级别肺神经内分泌肿瘤(19份)、肺腺癌(69份)、肺鳞癌(57份)活检标本及肺小细胞癌(9份)、肺腺癌(48份)和间皮细胞增生(23份)细胞学样本。结果:1) INSM1在肺小细胞癌,肺大细胞神经内分泌癌和低级别肺神经内分泌肿瘤中的阳性率分别为90.5%(38/42),66.7%(4/6),94.7%(18/19),显著高于肺腺癌(2.9%,2/69)、肺鳞癌(1.8%,1/57)中的阳性率(均P<0.01)。2)INSM1在肺小细胞癌细胞学样本中全部呈阳性表达,显著高于肺腺癌(2.1%, 1/48)和反应性间皮细胞增生(0,0/23)中的阳性率(均P<0.01)。3) INSM1免疫组织化学染色在32份活检和细胞学配对的标本中检测结果具有高度一致性(κ=1)。4) INSM1在肺神经内分泌肿瘤中的敏感性为90.1%(64/71),显著高于突触小泡蛋白(synaptophysin,Syn)和嗜铬粒蛋白A (chromogranin A,Cg A)(均P<0.001)。5)INSM1在肺神经内分泌肿瘤中的特异性为97.6%,显著高于Sy n(P<0.05),但是与Cg A相比差异无统计学意义(均P>0.05)。结论:INSM1在肺神经内分泌肿瘤中高表达,是鉴别诊断肺神经内分泌肿瘤比较可靠的标志物。     

泪囊鼻腔吻合术失败原因和再次手术探讨

目的:探讨泪囊鼻腔吻合术失败的原因及再次手术治疗的临床效果。方法:对17例17眼泪囊鼻腔吻合术失败者再次手术治疗,分析首次手术失败的原因,探讨再次手术的技巧、疗效。结果:术后随访4～12mo,17例再次手术均取得成功。泪囊鼻腔吻合术失败的主要原因系骨孔过小、位置不当,泪囊壁未完全切开、下端盲袋,前后瓣接触粘连。结论:掌握手术技巧、要点,正确处理首次出现的问题,再次手术能取得成功。