创伤后应激障碍大鼠海马和杏仁核中神经元凋亡及Caspase-9的表达及其意义

目的:探讨创伤后应激障碍(PTSD)大鼠海马和杏仁核神经元凋亡及Caspase-9表达水平的变化,阐明PTSD大鼠海马与杏仁核体积异常的原因。方法:将100只雄性Wistar大鼠随机分为对照组(20只)和PTSD组(80只),利用改良的单一连续应激(SPS&S)制作PTSD大鼠模型。采用Morris水迷宫实验和僵立行为实验检测大鼠行为学表现;TUNEL方法检测大鼠海马和杏仁核神经元凋亡阳性细胞率;流式细胞术(FCM)检测大鼠海马和杏仁核神经元凋亡率;Western blotting法检测大鼠海马和杏仁核中Caspase-9表达水平。结果:PTSD组大鼠逃避潜伏期(EL)和僵立行为百分比显著高于对照组(P<0.05);PTSD后1、4、7和14d大鼠海马和杏仁核TUNEL阳性细胞和凋亡率高于对照组(P<0.05),28d与对照组比较差异无统计学意义(P>0.05);PTSD后1、4、7和14d大鼠海马和杏仁核Caspase-9表达水平高于对照组(P<0.05),28d与对照组比较差异无统计学意义(P>0.05)。结论:PTSD大鼠海马和杏仁核神经元凋亡增加,Caspase-9表达水平上调,提示其凋亡可能是引起海马与杏仁核体积异常的原因之一。     

瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能

BACKGROUND:In recent years, it has been a hot topic that stem cell transplantation is used to improve cardiac insufficiency after acute myocardial infarction by inducing regeneration of cardiomyocytes in the infarction regions. OBJECTIVE:To observe the effect of rosuvastatin combined with umbilical cord blood mesenchymal stem cells transplantation on rat cardiac function after acute myocardial infarction. METHODS:Forty-five Sprague-Dawley rats were enrolled to prepare myocardial infarction models by ligaturing the left anterior descending coronary artery. Then they were equivalently divided into model group, transplantation group and combination group. At 7 days after modeling, rats in the combination group were given injection of 300 μL umbilical cord blood mesenchymal stem cells (15.0×10⁸) via the tail vein and by gavage once a day for 28 days with 1 mg/kg rosuvastatin; rats in the transplantation group and model group were injected with 300 μL umbilical cord blood mesenchymal stem cell suspension through the tail veins or the same amount of LG-DMEM medium, respectively, followed by intragastrical administration of the same amount normal saline. At 5 weeks after modeling, indexes of cardiac function, level of plasma Lp-PLA2 and heat shock protein 70 in the infarction regions were detected by color Doppler ultrasound, enzyme-linked immunosorbent assay and western blot assay, respectively. In addition, pathological changes of myocardial tissues were observed using hematoxylin-eosin staining. RESULTS AND CONCLUSION:Left ventricular ejection fraction and left ventricular end-systolic pressure were significantly higher in the combination group than in the transplantation group as well as higher in the transplantation group than the model group (P < 0.05); compared with the transplantation group, left ventricular end-diastolic pressure was significantly decreased in the combination group, but significantly increased in the model group (P < 0.05); the number of cardiomyocytes in the infarction regions was significantly higher in the combination group than the other groups. Additionally, expression of heat shock protein 70 in the infarction regions was significantly increased in the combination group (P < 0.05). To conclude, rosuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation can significantly improve rat cardiac function after myocardial infarction.     

骨髓间充质干细胞移植对胃癌模型大鼠肿瘤细胞的影响

BACKGROUND:Bone marrow mesenchymal stem cells can migrate into tumor tissues, as reported in recent studies. OBJECTIVE:To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cells on proliferation and differentiation of gastric cancer cell line SGC7901 in rats. METHODS:Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cell line SGC7901, and then, in cell transplantation group, each rat underwent an intraperitoneal injection of 1×107 bone marrow mesenchymal stem cells. After transplantation, the targeting ability of bone marrow mesenchymal stem cells was detected using fluorescent DiI labeling. Cyclin D2 mRNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cells was observed by in situ terminal labeling method. RESULTS AND METHODS: Bone marrow mesenchymal stem cells successfully migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cell transplantation group were significantly higher than those in the model group (P < 0.05). It was observed that the number of apoptotic cancer cells was significantly reduced in the cell transplantation group compared with the model group (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cells.     

外固定减张结合骨块缝合治疗髌骨下极骨折

髌骨下极骨折是发生于髌骨远端的骨折,为髌骨骨折中较特殊类型的关节内骨折,多为粉碎性骨折,可以带有少许关节软骨面,手术方式的选择直接关系到膝关节功能的恢复.自2006年4月至2008年4月,采用体外减张固定结合骨块缝合手术治疗18例髌骨下极骨折,效果良好,现报道如下.     

创疡贴在烧伤残留创面中的应用

目的 总结创疡贴对烧伤残留创面的临床疗效.方法 回顾性收集了过去两年应用创疡贴治疗烧伤残留创面的病历资料,进行归纳,分析创面愈合时间、止痛效果、创面感染菌种及随访情况.结果 72例残留创面的治愈率为96%,平均愈合时间为15.5 d±2.8 d,感染得到有效控制,且有止痛效果.结论 创疡贴利于烧伤残留创面的愈合,能有效控制创面感染.     

益气化瘀冲剂防治大鼠早期心力衰竭的作用机制

目的探讨益气化瘀冲剂防治大鼠早期心力衰竭的作用机制。方法从70只SD大鼠中随机取10只为空白对照组,余60只均采用腹主动脉缩窄术制备早期心力衰竭大鼠模型。造模大鼠6周内死亡20只,存活40只。将存活的40只大鼠随机分为4组,即模型对照组、贝那普利对照组及益气化瘀冲剂高、低剂量组,各10只。造模后6周开始给药,时间8周。空白对照组、模型对照组均予蒸馏水1 mL/(100 g.d)灌胃;贝那普利对照组予贝那普利混悬液0.36 mg/(100 g.d)灌胃;益气化瘀冲剂高、低剂量组分别予益气化瘀冲剂4.0、1.0 g/(100 g.d)灌胃。检测各组大鼠血流动力学指标及左心室质量指数。结果模型对照组大鼠血流动力学指标与空白对照组比较差异均有统计学意义(P<0.05,P<0.01),提示造模成功。益气化瘀冲剂高剂量组平均动脉压(MBP)、左室内压上升最大速率及左室内压下降最大速率与贝那普利对照组比较差异均有统计学意义(P<0.05)。益气化瘀冲剂高、低剂量组左室内压上升、下降最大速率比较差异有统计学意义(P<0.05)。贝那普利对照组及益气化瘀冲剂高、低剂量组左心室质量、左心室质量指数与模型对照组比较差异均有统计学意义(P<0.05,P<0.01)。结论益气化瘀冲剂能抑制大鼠心室重构的发展,在改善心力衰竭大鼠血流动力学方面,高剂量益气化瘀冲剂优于贝那普利;在改善左心室质量、质量指数方面,益气化瘀冲剂与贝那普利作用相似,且高剂量益气化瘀冲剂作用优于低剂量益气化瘀冲剂。     

消癥丸治疗子宫肌瘤400例临床观察

目的观察消癥丸治疗子宫肌瘤的临床疗效。方法将600例子宫肌瘤患者随机分为2组。治疗组400例予消癥丸口服治疗,对照组200例予桂枝茯苓丸口服治疗。2组均以3个月为1个疗程,1个疗程后观察临床疗效及2组治疗前后血液流变学指标变化。结果治疗组总有效率97%,对照组总有效率82%2,组总有效率比较差异有统计学意义（P〈0.05）,治疗组疗效优于对照组。治疗组治疗后全血黏度（低切）、全血黏度（高切）、红细胞聚集指数、血浆黏度及红细胞比容均低于本组治疗前（P〈0.05）;2组治疗后全血黏度（低切）、全血黏度（高切）、红细胞聚集指数、血浆黏度及红细胞比容比较差异均有统计学意义（P〈0.05）,治疗组均低于对照组。结论消癥丸治疗子宫肌瘤疗效显著,价格便宜,且无毒副作用。     

青少年颈椎病临床特征、X线特点及诊疗对策探讨

目的：通过对138例15~30岁青少年颈椎病患者临床特征及颈椎X片的分析,探讨诊疗对策。方法：统计临床症状和体征;均行DR-X线摄影机摄颈椎正位、中立位侧位、张口位平片检查,按照颈椎病诊断标准测量分析。结果：本组临床症状体征以颈型、椎动脉型、交感神经型为主;本组X片改变以颈椎小关节的紊乱引起颈椎失稳为主,以曲度的变直/反曲和椎体的轻度旋转错位为最突出的表现。结论：青少年颈椎病的眩晕/头晕症状与交感神经激惹引起椎动脉痉挛有关;青少年颈椎病是一种心身疾病;因X片特点是脊柱在水平、垂直、及旋转3D空间的复杂错位,故手法复位应是最主要的治疗方法,但扳法的应用需谨慎;中药的治疗不仅需要舒筋活络、活血通脉,还须注意对情志的调整。     

胃康宁颗粒治疗慢性萎缩性胃炎癌前病变40例临床观察

目的观察辛开苦降法对慢性萎缩性胃炎癌前病变患者的治疗作用。方法将80例慢性萎缩性胃炎癌前病变患者随机分为治疗组和对照组各40例,治疗组予胃康宁颗粒口服,每次2g,每日3次;对照组予胃复春片口服,每次4片,每日3次,两组均治疗6个月。观察两组患者综合临床疗效、胃镜疗效及治疗前后病理表现。结果治疗组综合疗效总有效率为87.5%,对照组总有效率82.5%,两组疗效比较差异有统计学意义(P<0.05)。治疗组胃镜疗效总有效率为75.0%,对照组总有效率45.0%,两组疗效比较差异有统计学意义(P<0.05)。治疗组治疗后胃黏膜炎症、萎缩、肠上皮化生均有明显改善(P<0.05),异型增生无明显改善(P>0.05)。结论以辛开苦降立法的胃康宁颗粒能明显缓解慢性萎缩性胃炎癌前病变患者的临床症状,减轻胃黏膜炎症、萎缩和肠上皮化生。     

托毒益气养阴汤治疗肛周坏死性筋膜炎(毒盛正虚证)的临床研究

目的 观察托毒益气养阴汤治疗肛周坏死性筋膜炎(毒盛正虚证)的临床疗效.方法 将66例患者随机分为治疗组与对照组各33例,对照组采用西医常规治疗,治疗组在对照组基础上采用内服托毒益气养阴汤治疗.两组均治疗2周.结果 两组治疗后创面疼痛、病变范围、全身症状、坏死性筋膜炎实验室风险指数(LRINEC)评分及白细胞计数(WBC...