建立一种新的多药耐药的诱导方法

目的建立裸鼠原位肝癌耐药模型。方法培养肝癌细胞系HepG2,建立裸鼠的皮下肿瘤,形成“供瘤鼠”。开腹直视下将瘤块种植于裸鼠的肝包膜下建立原位肝癌模型,通过表阿霉素间歇腹腔化疗,建立裸鼠原位肝癌耐药模型。用体检、B超、CT、剖腹探查监测肝内瘤块生长情况。用逆转录聚和酶链反应(RT-PCR)和免疫组织化学方法检测肿瘤耐药基因mdrl-mRNA和p-gp蛋白的表达。结果(1)模型建立无手术死亡(0/25),种植成瘤率为88%(22/25),补种3例全部成功,耐药诱导成功率为80%(16/20);(2)诱导组mdrl-mRNA和P-gp蛋白的表达均明显高于对照组,分别约是对照组的23倍和13倍。结论成功地建立了与临床肝癌相似的裸鼠原位肝癌耐药模型,为进一步研究肝癌多药耐药基因的诊断和逆转提供了良好的动物平台。     

血小板活化因子、癌胚抗原、糖类抗原724在结直肠癌中的表达及与临床病理特征的关系

目的:探讨血小板活化因子(PAF)、癌胚抗原(CEA)、糖类抗原724(CA-724)在结直肠癌(CRC)患者术前血清中的阳性表达率及与临床病理特征的关系、发病的独立危险因素。方法:选取2019年12月至2021年10月于包头医学院第一附属医院就诊的CRC患者75例为CRC组,另选取同时期在本院体检的健康志愿者75例为对照组。采用酶联免疫吸附(ELISA)法分别检测CRC患者术前及对照组的晨空腹外周静脉血PAF含量;回顾性分析上述入组患者术前血清CEA、CA-724含量,进行统计分析。结果:(1)CRC组PAF、CEA、CA-724单独检测和联合检测的阳性率分别为25.33%、42.67%、30.67%、74.67%,均高于对照组,且联合检测的阳性率均高于单独检测指标的阳性率,组间差异均具有统计学意义(P<0.05);(2)单因素方差分析结果显示,血清CEA表达水平的影响因素为患者的淋巴结转移(P<0.05);血清CA-724表达水平的影响因素为患者的年龄(P<0.05);(3)二元Logistic回归分析结果显示,PAF表达在临床病理特征之间的差异无统计学意义(P&...     

环氧酶-2抑制剂对关节炎大鼠小肠黏膜的影响

目的 探讨环氧酶-2抑制剂对关节炎大鼠小肠黏膜的影响。方法 将32只雄性SD大鼠随机分为空白对照组、造模对照组、空白给药组和造模给药组,造模对照组、造模给药组给予弗氏完全佐剂右后足跖注射,空白对照组、空白给药组注射等量生理盐水。1周后造模给药组、空白给药组给予选择性环氧酶-2抑制剂塞来昔布（溶于1%甲基纤维素）灌胃28 d,空白对照组、造模对照组给予等量溶剂。处死大鼠后观察小肠黏膜大体损伤、病理评分,酶联免疫吸附试验检测末段小肠前列腺素浓度。结果 造模对照组与造模给药组模型复制第7天的足周径比较,差异无统计学意义（P>0.05）,但两组分别与空白对照组和空白给药组比较,差异有统计学意义（P <0.05）,空白对照组与空白给药组比较,差异无统计学意义（P>0.05）。空白对照组与造模对照组小肠黏膜损伤面积比较,差异有统计学意义（P <0.05）,空白给药组与空白对照组比较,差异有统计学意义（P <0.05）。造模对照组、空白给药组和造模给药组组间小肠黏膜病理评分比较,差异无统计学意义（P>0.05）,且空白对照组与其他3组比较,差异有统计学意义（P &...     

骨髓间质干细胞吲哚胺2，3-双加氧酶活性对T淋巴细胞的影响(英文)

目的: 探讨骨髓间质干细胞（MSCs）吲哚胺2，3-双加氧酶（IDO）活性对抑制T淋巴细胞应答反应的影响。方法: 从人骨髓中分离培养间质干细胞,通过其形态特点、表面标志及多向分化能力检测进行鉴定。以浓度为2×105 U/L的 IFN-γ对分离的MSCs诱导18 h，检测MSCs上IDO mRNA和IDO蛋白表达。将经过IFN-γ 2×105 U/L诱导的MSCs预先接种在培养板中，再建立混合淋巴细胞培养(MLR)体系，利用MTT法检测T淋巴细胞增殖率，并用反相高效液相色谱法检测IDO活性。结果: IFN-γ能诱导MSCs上IDO mRNA和IDO蛋白的表达；MSCs的IDO活性抑制MLR体系中T淋巴细胞增殖率。结论： 经IFN-γ刺激后的MSCs在体外可抑制异体T淋巴细胞的免疫应答，IDO活性参与了这种免疫抑制作用的发挥。     

Association between bariatric surgery and anemia

ObjectivesSeveral studies have recently conducted to investigate the link between anemia and bariatric surgery (BS). The results from these studies, however, were inconsistent. Therefore, a meta-analysis was conducted to determine whether BS for weight loss is a risk factor for anemia.MethodsStudies were identified relevant papers published a cutoff date of May 2022 for meta-analysis by searching four electronic databases (PubMed, EMBASE, Web of Science and Cochrane Library). Pairs of authors conducted title and abstract, full-text assessment, data extraction and risk of bias assessment, and quality of articles. Random-effects models were used to estimate the pooled effect size and evaluated the overall quality of evidence. The sources of heterogeneity were assessed with I². The Newcastle-Ottawa scale (NOS) was used to assess the methodologic quality of selected studies. The publication bias was assessed using funnel plots and Egger's test statistics.ResultsIn all, eight studies comprising 3150 patients were finally included in the final study analysis. Of these, pooled analysis of eight studies detecting association between anemia and BS for weight loss yield a statistically significant correlation (SMD ?1.12 (?1.46—?0.78, I² = 93%). Subgroup analyses were performed according to postoperative follow-up time and operation method. Before 24 months postoperatively, no patient had a significant decrease in hemoglobin. More than 24 months after BS, patients show a significant reduction in hemoglobin. In addition, the hemoglobin level was not changed after sleeve gastrectomy (SG) and adjustable gastric banding (AGB) (SMD ?0.19 (?1.04—0.67, I² = 0% for SG; SMD ?0.30 (?0.82—0.22 for AGB), but the hemoglobin level was significantly reduced after Roux-en-Y gastric bypass (RYGB) (SMD ?1.19 (?1.52—?0.87, I² = 94.2%).ConclusionThis current meta-analysis found that at 24 months after RYGB, the patient's hemoglobin level was significantly reduced, increasing the risk of anemia. Therefore, attention should be paid to the occurrence of anemia after BS. A large, placebo-controlled, multi-center study is needed to clarify the role of BS for weight loss in this patient anemia.     

大数据背景下公立医院医疗服务审计模型构建及应用*

为构建大数据背景下公立医院医疗服务审计模型,文章以中国科学技术大学附属第一医院为样本医院,提出了审计模型设计思路,分别梳理了医疗服务价格、药品购销存、耗材购销存3类审计的相关规则和建模步骤,并应用所建模型对业务数据进行了分析处理,及时发现了医院潜在风险、业务疑点,提高了内部审计精准度、时效性以及风险预测能力,促进了医院精细化管理和高质量发展。     

Penetration moxibustion for postpartum subinvolution of uterus: Two case reports and mechanism analysis: 透灸法成功治疗产后子宫复旧不良:两则病例报告及机制分析

This article introduced the application of penetration moxibustion in successfully treating two cases of postpartum subinvolution of uterus. The main complaint of one case was increasing volume of abnormal vaginal bleeding for five days after delivery. The bleeding stopped after the patient received 7 times treatment of warming needle and penetration moxibustion on Zhōngjí (中极CV3), Guānyuán (关元CV4) and Qìhăi (气海CV6). The other case complained about persistent abdominal pain for 15 days, which was worsening for the recent 2 days. The symptom of abdominal pain was relieved after the penetration moxibustion was applied on CV4, CV6 and Zǐgōng (子宫 EX-CA1) for 5 times for the case. Penetration moxibustion can relieve abdominal pain during the postpartum period, accelerate lochia discharge, and recovery of menstrual and uterine cavity. Àiyè (艾叶 Artemisiae Argyi Folium) was proved to have antibacterial, hemostatic, and analgesic effects. The warming function and radiation features of moxibustion can help to alleviate spasm of the smooth muscle in the uterus and enhance the metabolism of the uterine tissue by expanding microvessels and improving the circulations of both blood and lymph. It can also activate cells in the uterine tissue by increased temperature in order to enhance the decomposition and elimination of pathological wastes, such as inflammation and hematoma. In addition, it can also relieve pelvic pain by inhibiting neuronal excitability.