Characterization of a precipitating antigen detected in the serum of patients with viral hepatitis

During a search for the aetiological agent of non-A non-B hepatitis, a precipitating antigen was detected in the sera of some patients during the acute phase of their illness. The antigen was detected by agar gel diffusion using antibody from convalescent sera obtained from patients with non-A non-B hepatitis, and from haemophiliac sera. The antigen was usually detected early in the patient's illness, disappearing as liver function tests returned to normal. In some patients specific antibody appeared during the convalescent phase of the disease. The antigen does not appear to be specific for non-A non-B hepatitis, as it could be detected with similar frequency in patients with hepatitis A or hepatitis B and some patients with other liver disorders. Biochemical and biophysical studies suggest that the antigen is probably an abnormal lipoprotein produced as a result of acute liver damage.     

Identification of novel regions of allelic loss from a genomewide scan of esophageal squamous-cell carcinoma in a high-risk Chinese population

Esophageal cancer is one of the most common fatal cancers worldwide. Deletions of genomic regions are thought to be important in esophageal carcinogenesis. We conducted a genomewide scan for regions of allelic loss using microdissected DNA from 11 esophageal squamous-cell carcinoma patients with a family history of upper gastrointestinal tract cancer from a high-risk region in north central China. Allelic patterns of 366 fluorescently labeled microsatellite markers distributed at 10-cM intervals over the 22 autosomal chromosomes were examined. We identified 14 regions with very high frequency (>/= 75%) loss of heterozygosity (LOH), including broad regions encompassing whole chromosome arms (on 3p, 5q, 9p, 9q, and 13q), regions of intermediate size (on 2q, 4p, 11p, and 15q), and more discrete regions identified by very high frequency LOH for a single marker (on 4q, 6q, 8p, 14q, and 17p). Among these 14 regions were 7 not previously described in esophageal squamous-cell carcinoma as having very high frequency LOH (on 2q, 4p, 4q, 6q, 8p, 14q, and 15q). The very high frequency LOH regions identified here may point to major susceptibility genes, including potential tumor suppressor genes and inherited gene loci, which will assist in understanding the molecular events involved in esophageal carcinogenesis and may help in the development of markers for genetic susceptibility testing and screening for the early detection of this cancer. Genes Chromosomes Cancer 27:217-228, 2000. Published 2000 Wiley-Liss, Inc.     

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum: comparison with results of other viral markers

A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients’ sera, respectively. The detection rate of the N protein during days 11–15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.     

血脂测定结果的临床可接受性分析

目的:通过对不同检测系统血脂测定结果的偏倚评估,分析各系统间结果的可比性和临床可接受性。方法:参照CLSI文件相关要求,以可溯源的检测系统为目标检测系统,测定总胆固醇(TCH)、甘油三酯(TG)、载脂蛋白A1(APOA1)、载脂蛋白B(APOB)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C),对本院3个不同检测系统进行朗道质控物(水平2和水平3)各测定21次和测定新鲜血清标本40份。结果:朗道质控物和新鲜血清标本TCH、TG、APOA1、APOB、HDL-C、LDL-C浓度在系统间的差异均有显著性(P<0.05)。除LDL-C在各检测系统间的相关系数为0.7859~0.9259之间外,其他项目各系统间的相关系数均大于0.975。各检测系统测定TCH、TG的不精密度CV均小于5%,其他项目的不精密度CV均小于10%;以可溯源检测系统1为目标检测系统,临床可接受性能评价:TCH、LDL-C在检测系统2和检测系统3超出临床接受范围,HDL-C在检测系统2超出临床接受范围,其他项目临床均可接受。结论:3个不同检测系统测定TG、APOA1、APOB结果具有可比性;测定HDL-C结果具有部分可比性,TCH、LDL-C结果不具有可比性,需采取整改措施。     

Study on structure-property relationship of polyimide blends, 2. Structure and miscibility of polyimide PBPI-E/PTI-E blends

The structure and miscibility of polyimide PBPI-E/PTI-E blends were studied by wide- and small-angle X-ray scattering and dynamic mechanical analysis, where PBPI-E is a biphenyldianhydride-based polyimide, and PTI-E is a polyimide from 4,4′-thiodiphthalic anhydride and 4,4′-oxydianiline. The results obtained show that there exists a paracrystalline structure in the blends with high content of PBPI-E, but this does not affect the miscibility of the blends. The blends are miscible over the entire composition range, since only one T_g was observed for each blend. Meanwhile, the segregation of PTI-E during crystallization of PBPI-E in the blends is interlamellar.     

非典型肺炎病人免疫功能分析

目的 :动态监测非典型肺炎 (SARS)病人免疫功能 ,为临床诊疗提供帮助。方法 :采用回顾性及随访研究方法 ,对本院收治 10 3例SARS病人分别在入院初期、中期、高峰期、恢复期和随访时作C反应蛋白 (CRP)、免疫球蛋白、补体、纤维蛋白原 (Fib)、血沉 (ESR)、和CD4、CD8等免疫功能检查 ,并进行描述性分析及方差分析。结果 :从发病初期至恢复期CRP、Fib、ESR均有程度不同的升高 ,以CRP异常率最高。总蛋白、白蛋白、白蛋白 /球蛋白比值在疾病过程呈下降趋势。在SARS疾病过程中和随访时存在免疫球蛋白变化、补体消耗和CD4、CD8细胞减少。结论 :SARS病人免疫功能可能低于正常人。其免疫功能的检测有利于协助诊断和疗效判断     

锂与缺碘性甲状腺肿发病关系的实验研究

实验用Wistar大鼠125只。随机分成五组。动物实验期为20周。结果显示:单纯低碘或单纯高锂的大鼠均表现为增生性甲状腺肿的组织学改变及血清T_4降低、T_3升高;前者甲状腺上皮细胞增生较后者显著,后者甲状腺滤泡腔内胶质含量较多。低碘合并高锂摄入的大鼠,其甲状腺病变较单纯低碘或单纯高锂大鼠严重,并呈明显的胶性甲状腺肿组织学改变,血清T_4降低、T_3正常。可见高锂不仅有致甲肿作用,而且当它与低碘并存时,将加重甲状腺的病变,并使其向胶性甲状腺肿发展。     

共聚焦激光扫描显微镜(MRC600)活体观测川芎嗪和去甲基肾上腺素对休克状态下家兔大脑皮质内微循环的影响

目的 :共聚焦激光扫描显微镜活体观测川芎嗪和去甲基肾上腺素对休克状态下家兔大脑皮质内微循环的影响。方法 :在开放颅窗的家兔模型上 ,荧光素标记血浆 ,罗丹明 6G标记WBC ,用共聚焦激光扫描显微镜活体观测川芎嗪和去甲基肾上腺素对休克状态下家兔大脑皮质内微循环的影响 ,并经图像分析系统测量数据 ,用SAS软件包进行统计学分析。结果 :①川芎嗪抗休克效果优于去甲基肾上腺素 ;②去甲基肾上腺素在休克状态下对口径为 60 .15 μm的动脉血管处未引起明显的血管运动 ,而川芎嗪能引起血管运动 ,尤以大剂量川芎嗪引起强烈的血管运动 ;③川芎嗪和去甲基肾上腺素增加或保持血液缘流厚度不变 ,可能是两者抗休克机制发挥作用的途径之一 ;④川芎嗪和去甲基肾上腺素引起血管运动 ,尤以中小血管处明显。结论 :川芎嗪抗休克效果优于去甲基肾上腺素。川芎嗪和去甲基肾上腺素增加或保持血液缘流厚度不变 ,可能是两者抗休克机制发挥作用的途径之一     

局部晚期肺癌外科手术治疗(附18例报告)

目的为提高晚期肺癌患者的外科手术切除率及提高手术疗效，总结18例晚期肺癌患者手术治疗中切开心包处理肺静脉或切除部分左心房的经验。方法对18例晚期肺癌患者施行肺叶或全肺切除时，在心包内处理肺静脉或切除部分左心房。左肺下叶切除5例、左全肺切除3例、右肺中下叶切除4例、右肺下叶切除4例、右全肺切除2例；术中发现13例患者的肺静脉根部有癌侵犯或有癌栓，在心包外元法常规处理肺静脉，在切开心包后可较满意地处理肺静脉，切开心包后发现其中有9例明显有左心房侵犯，行肺切除的同时切除部分左房；5例虽可在心包外处理，但癌肿离肺静脉根部太近处理肺静脉困难，而切开心包后处理肺静脉很轻松。结果18例均无手术死亡，术后有2例发生心律失常，2例并发肺炎，1年生存率61．2％（11／18）、3年生存率38．8％（7／18）、2例生存〉5年。结论晚期肺癌累及肺静脉根部或左心房时应切开心包处理肺静脉或同时切除部分左心房以达到肿瘤学上的根治性切除，从而延长病人的寿命。     

应用多层螺旋CT对耳硬化症的术前评估价值

目的:评估MDCT在术前耳硬化症诊断中的作用。方法:收集经临床证实为耳硬化症、且CT扫描采用螺旋扫描病例共18例,采集的数据传输到4.1工作站,进行多平面重建。结果:18例共36耳显示异常:单独前庭窗异常18耳,表现为前庭窗扩大或狭小,周围骨质密度降低或镫骨底板板密度增高;前庭窗及蜗窗同时受累共8耳;主要累及耳蜗周围迷路骨质10耳,表现为耳蜗骨迷路边缘不整,呈条片状密度减低或双环征。MPR交互重建充分显示了所有重要解剖结构的位置、形态:其中3耳为颈静脉球高位。结论:MDCT可以正确显示耳硬化症病灶的细微改变,提供详细的术前诊断信息;结合MPR技术可全面观察病变范围,并充分显示颞骨内重要解剖结构的位置和形态。对于手术方案的设计、防止手术并发症和提高疗效等具有重要意义。