基因枪转导K-ras突变反义基因对人胰腺癌细胞生长曲线的影响

目的探讨基因枪转导K-ras突变反义基因对胰腺癌细胞生长的影响。方法利用基因枪技术,将反义基因转导入宿主细胞,通过活细胞计数试剂盒Cell Counting Kit-8和Thermo Multiskan Ascant酶标仪观察K-ras突变特异性反义基因转导后对AsPC-1、MiaPaCa-2和BxPC-3三种人胰腺癌细胞的生长曲线变化情况。结果转导K-ras突变特异性反义基因后,突变型AsPC-1和MiaPaCa-2胰腺癌细胞的生长曲线明显向右下移动。结论转导K-ras突变特异性反义基因后,具有突变型K-ras基因的胰腺癌细胞系的生长都受到明显抑制。     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

目的探讨脑星形细胞瘤中TIP30、血管内皮生长因子（VEGF）、CD34的表达情况及其病理意义。方法采用免疫组织化学Elivision plus两步法,检测19例正常脑组织及71例星形细胞瘤组织中TIP30、VEGF及CD34的表达。结果TIP30在19例正常脑组织神经胶质细胞及神经元胞质内均呈阳性表达;71例星形细胞瘤组织中,TIP30总阳性表达率为33．80％,随星形细胞瘤分化程度的降低,TIP30的表达逐渐降低,Ⅱ、Ⅲ和Ⅳ级星形细胞瘤中TIP30阳性表达率分别为52％、34．78％和13．04％。高级别星形细胞瘤（Ⅲ级＋Ⅳ级）中TIP30的阳性表达率显著低于其在低级别星形细胞瘤（Ⅱ级）中的阳性表达率（x^2=5．71,P〈0．05）;VEGF及由CD34确定的MVD在星形细胞瘤中表达均高于正常脑组织,且随星形细胞瘤病理级别升高表达逐渐增高;星形细胞瘤中TIP30与VEGF强阳性表达呈负相关关系（r=-0．428,P〈0．05）。而TIP30与MVD无明显相关性（r=-0．065,P〉0．05）。结论TIP30在正常脑组织中阳性表达率高于其在星形细胞瘤中阳性表达率,且随星形细胞瘤病理级别升高表达显著下降;星形细胞瘤中,VEGF的表达与TIP30的表达呈明显的负相关关系。     

p27蛋白在甲状腺肿瘤中表达的研究

目的探讨 p2 7蛋白在甲状腺肿瘤组织中的表达及意义。方法应用免疫组织化学高碘酸钠 (sodiumperiodate,SP)法对 36例甲状腺癌和 10例甲状腺瘤组织进行 p2 7检测。 结果p2 7蛋白在甲状腺癌组织中的表达率为 72 .2 % ,甲状腺瘤的表达率为 2 0 .0 % ,其表达率随组织分化程度的下降而下降 (P <0 .0 5 )。结论 p2 7蛋白与甲状腺肿瘤的生物学行为有关 ,有助于良恶性甲状腺肿瘤的鉴别 ,可作为甲状腺肿瘤分化程度的 1个参考指标。     

香加皮三萜类化合物抑制食管癌Eca109细胞裸鼠成瘤及其机制

目的： 研究香加皮三萜类化合物（triterpenes compound extracted from cortex periplocae,TCCP）对荧光素酶（luciferase）标记的人食管鳞癌细胞株Eca109（Eca109-luc细胞）在裸鼠体内成瘤的作用及其机制。 方法： Eca109-luc细胞经皮下接种裸鼠,构建Eca109-luc细胞裸鼠移植瘤模型,观察TCPP治疗后移植瘤体积和质量的变化,应用活体成像系统观察移植瘤生长情况,H-E染色观察移植瘤组织形态学变化,流式细胞仪检测移植瘤细胞的凋亡,Western blotting检测移植瘤组织中survivin的表达。 结果： TCPP在体内能明显抑制裸鼠Eca109-luc移植瘤的生长,移植瘤体积和质量明显减少,抑瘤率为40.7%。TCPP 治疗组小鼠移植瘤组织出现明显炎性细胞浸润及肿瘤细胞坏死,移植瘤细胞的凋亡率明显高于大豆油对照组（P<005）,移植瘤组织中survivin蛋白表达水平明显下降（P<0.05）。 结论： TCCP在体内能抑制人食管癌Eca109细胞的生长,其作用机制可能与下调survivin的表达诱导肿瘤细胞凋亡有关。     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

大鼠睾丸纤维化模型睾丸内细胞增殖的实验研究

目的:探讨大鼠睾丸纤维化模型睾丸内各种细胞的增殖情况。方法采用免疫组化ElivisionTM Plus二步法,观察增殖细胞核抗原在纤维化睾丸中的表达。结果纤维化睾丸内Leydig细胞、成纤维细胞和血管内皮细胞均显著增多。结论各种细胞的增殖是睾丸纤维化和生精障碍的主要原因。     

二甲双胍治疗后多囊卵巢综合征大鼠子宫内膜Hoxa10的表达及意义

目的:探讨二甲双胍治疗前后多囊卵巢综合征(PCOS)大鼠子宫内膜组织中Hoxa10及胰岛素(INS)、INS受体(INS-R)蛋白表达的变化. 方法: 选取85日龄SD雌性大鼠54只,随机分为:①对照组(20只);② PCOS组(17只);③二甲双胍治疗组(17只). 后两组先参照Poretsky L等方法复制PCOS大鼠模型,造模成功后,PCOS组喂服安慰剂,治疗组喂服二甲双胍,14 d后断头处死各组大鼠并取材. 采用免疫组织化学ElivisionTM Plus两步法检测三组子宫内膜Hoxa10及INS,INS-R蛋白的表达. 并取外周血测定INS和睾酮含量. 结果: PCOS组大鼠子宫内膜Hoxa10和INS-R表达下降(P＜0.01, P=0.012),INS表达增加(P=0.003);二甲双胍治疗后子宫内膜Hoxa10表达增加(P＜0.01),INS水平下降(P=0.043),但均低于对照组水平(P＜0.01, P=0.037);INS-R无明显变化;治疗后血INS和血睾酮水平均下降(P＜0.01, P=0.048). 结论: PCOS大鼠子宫内膜可能存在INS抵抗和容受性下降,治疗后可部分逆转此变化.     

调节性T细胞在原发性干燥综合征中的异常表达

目的 观察调节性T细胞在原发性干燥综合征(pSS)患者外周血和唇腺中的表达.方法 选取23例pSS患者和15名健康对照组,通过流式细胞术检测2组之间外周血调节性T细胞的表达情况,采用免疫磁珠分选外周血CD4+T细胞,提取叉头样转录因子(Foxp3)的mRNA,进行实时荧光定量的聚合酶链反应(PCR)检测.选取23例pSS患者和5例除外pSS的原发性胆汁性肝硬化(PBC)患者,通过免疫组织化学方法分析2组之间唇腺组织中CD4+和Foxp3+T细胞的表达差异.采用t检验进行统计 学处理.结果 pSS组[(5±6)％]外周血Foxp3+T细胞的表达比健康对照组[(10±5)％]明显降低(t=2.190; P=0.036); Foxp3-mRNA的含量pSS组(0.08±0.05)与健康对照组(0.09±0.03)之间差异无统计学意义(t=0.695;P＞0.05);与PBC对照组唇腺中CD4+T细胞[(11±6)％]和Foxp3+T细胞[(3.2±1.1)％]比较,pSS患者唇腺中CD4+T细胞[(30±10)％]和Foxp3+T细胞[(10.7±5.8)％]表达增加(t=4.072,2.840;P均＜0.05).结论 调节性T细胞在pSS外周血表达降低,而在唇腺中的表达并未减少,二者之间表达不一致,Foxp3参与了pSS的发病.     

健脾止泻宁颗粒联合美沙拉嗪治疗溃疡性结肠炎中细胞因子与髓过氧化物酶的关系

目的观察健脾止泻宁颗粒联合美沙拉嗪对溃疡性结肠炎(UC)模型大鼠细胞因子以及结肠组织中髓过氧化物酶(MPO)水平的影响。方法 75只Wistar雄性大鼠采用随机数字表法随机分为5组:正常对照组、模型对照组、健脾止泻宁组、美沙拉嗪组和联合治疗组, 每组15只。采用大鼠疾病活动指数(DAI)评分评估大鼠肠炎症状严重程度的指标。酶联免疫吸附试验(ELISA)检测各组大鼠血清中细胞因子白细胞介素(IL)-1β、IL-4、IL-6、IL-8、IL-10、肿瘤坏死因子-α(TNF-α)、IL-18水平, 蛋白质印迹法(Western blot)检测结肠黏膜组织中MPO的表达。采用单因素方差分析进行统计学分析。结果与正常对照组比较, 模型组及各个给药治疗组DAI评分均增高, 差异有统计学意义(F=128.574, P<0.05);与模型组比较, 各治疗组DAI评分均降低, 差异有统计学意义(F=128.574, P<0.05)。与模型组比较, 健脾止泻宁组、美沙拉嗪组及联合用药组血清IL-1β、IL-6、IL-8、TNF-α和IL-18水平升高(F=90.948、92.243、132.4...